Antibacterial Activity of Methanol Extracts and Fractions from Kalanchoe crenata, Terminalia avicennioides and Sarcocephalus latifolius

Raymond, Mouokeu; Yimta, Foutse; Nguimatsia, François; Sedar, Njateng Guy; Di, Tamokou Jean de; Roge, Kuiate Jules

doi:10.5567/pharmacologia.2014.199.204

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Numerous active chemical components found in medicinal plant extracts have antibacterial effects. The MICs value has been used for evaluating the extract's antibacterial activity [13]. If the MIC values are less than 500 g/mL, the activity is regarded as significant, and when they range between 500 and 1500 g/mL, it is regarded as moderate.…”

Section: Discussionmentioning

confidence: 99%

Invitroanti-Staphylococcus aureusactivity of the methanol extract from Mitrephora thoreliiPierre’s branches and leavesin Lam Dong Province, Viet Nam

Quynh¹,

Phuc²,

Hoa³

et al. 2023

JIPBS

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We demonstrated that the methanol extract of Mitrephora thoreliiPierre's branches and leavesin Lam Dong, Vietnam is anti-Staphylococcus aureusstrain (Sta1) of Phenikaa University, Hanoi, Viet Nam and Staphylococcus aureusATCC 29213strain(Sta2) from Thermo Fisher Scientific, USA. Invitro anti-Sta1 and Sta2 activity of the methanol extract of Mitrephora thoreliiPierre's branches and leaves were not significantly different. With a rise in extract content, the inhibitory zone's created diameter grew as well. The extract content of 100 mg/mL demonstrated the greatest antibacterial activity with a 25 mm inhibition zone diameter against Sta1 and 24mm inhibition zone diameter against Sta2. Bacteria were not resistant to the extract at concentrations of 20; 80 and 100 mg/mL after 48, 72, and 96 hours. The lowest inhibitory concentration (MIC) was between 312.5 and 625 μg/mL. The minimum bactericidal concentration was found to be 1.25mg/mL.

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Section: Discussionmentioning

confidence: 99%

Invitroanti-Staphylococcus aureusactivity of the methanol extract from Mitrephora thoreliiPierre’s branches and leavesin Lam Dong Province, Viet Nam

Quynh¹,

Phuc²,

Hoa³

et al. 2023

JIPBS

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Parts, Period, and Extraction Solvents as Parameters Influencing Harungana madagascariensis Lam. ex Poir. (Hypericaceae) Antibacterial Activity

Tégaboué

Pouaha

Etame

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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The study of the conditions which affect the pharmacological activity of medicinal plants is the basis for the development of phytomedicine. This study aimed at identifying some optimal conditions for antibacterial activity of extracts of Harungana madagascariensis. The leaves, bark, and roots were harvested in Douala, Littoral Region of Cameroon, in August 2019 at three times of the day: early in the morning (6 a.m.), at midday (12 p.m.), and in the afternoon (6 p.m.). They were dried at room temperature and ground to give a fine powder. Each powder was macerated in methanol for 72 h and boiled in distilled water and palm wine. The obtained extracts were evaluated for their antibacterial activity by broth microdilution method on 31 clinical and 4 reference bacterial strains. The results show that the best extraction yield was obtained using the bark extracted from palm wine by decoction. The methanol bark extract was found to be more active than other solvents (8 and 512 µg/mL). In fact, the activity of the 6 a.m. extract was significant on 4 of the 35 strains tested (MIC< 100 µg/mL) and moderate on 27 of the 35 strains tested (100≤ MIC≤ 625 μg/mL). The activity of the noon extract was significant on 8 strains and moderate on 23 strains and, finally, the activity of the extract of 6 p.m. was significant on 9 strains and moderate on 25 strains. The extract harvested at 6 p.m. inhibited the growth of the 35 strains tested and revealed significant activity (MIC <100 μg/mL) on Staphylococcus aureus, Escherichia coli, Citrobacter freundii, Serratia marcescens, Salmonella enterica serovar typhi, and Yersinia enterocolitica, making this hour the best harvesting time. This study further confirms that the bark of H. madagascariensis is a potent antibacterial agent, being better still when harvested at 6 p.m. and extracted with methanol.

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Antimicrobial and cytotoxicity properties of the crude extracts and fractions of Premna resinosa (Hochst.) Schauer (Compositae): Kenyan traditional medicinal plant

Njeru

Obonyo

Nyambati

et al. 2015

BMC Complement Altern Med

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BackgroundPremna resinosa (Hochst.) Schauer also called “mukarakara” in Mbeere community of Kenya is used in the management of respiratory illness. In this study we investigated antituberculous, antifungal, antibacterial activities including cytotoxicity and phytochemical constituents of this plant.MethodsAntibacterial and antifungal activities were investigated by disc diffusion and micro dilution techniques. Antituberculous activity was investigated using BACTEC MGIT 960 system while cytotoxicity was analyzed by MTT assay on Vero cells (Methanolic crude extract) and HEp-2 cells (fractions). Finally, phytochemicals were profiled using standard procedures.ResultsP. resinosa had high antituberculous activity with a MIC of <6.25 μg/ml in ethyl acetate fraction. The antibacterial activity was high and broad spectrum, inhibiting both Gram positive and Gram negative bacteria. Dichloromethane fraction had the best antibacterial MIC of 31.25 μg/ml against Methicillin-resistant S. aureus while Ethyl acetate fraction had the highest zone of inhibition of 22.3 ± 0.3 against S. aureus. Its effects on tested fungi were moderate with petro ether fraction giving an inhibition of 10.3 ± 0.3 on C. albicans. The crude extract and two fractions (petro ether and methanol) were not within the acceptable toxicity limits, however dichloromethane and ethyl acetate fractions that exhibited higher activity were within the acceptable toxicity limit (CC50 < 90). The activity can to some extent be associated to alkaloids, flavonoids, terpenoids, anthraquinones and phenols detected in this plant extracts.ConclusionOur findings demonstrate that P. resinosa has high selective potential as a source of novel lead for antituberculous, antibacterial and antifungal drugs. Of particular relevance is high activity against MRSA, S. aureus, C. albicans and MTB which are great public health challenge due to drug resistance development and as major sources of community and hospital based infections.

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Antibacterial Activity of Methanol Extracts and Fractions from Kalanchoe crenata, Terminalia avicennioides and Sarcocephalus latifolius

Cited by 4 publications

References 6 publications

Invitroanti-Staphylococcus aureusactivity of the methanol extract from Mitrephora thoreliiPierre’s branches and leavesin Lam Dong Province, Viet Nam

Invitroanti-Staphylococcus aureusactivity of the methanol extract from Mitrephora thoreliiPierre’s branches and leavesin Lam Dong Province, Viet Nam

Parts, Period, and Extraction Solvents as Parameters Influencing Harungana madagascariensis Lam. ex Poir. (Hypericaceae) Antibacterial Activity

Antimicrobial and cytotoxicity properties of the crude extracts and fractions of Premna resinosa (Hochst.) Schauer (Compositae): Kenyan traditional medicinal plant

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