﻿Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™

Satria, Denny; Sofyanti, Ervina; Wulandari, Pitu; Fajarini, Fajarini; Pakpahan, Sri Dewi; Limbong, Stephanie Artha

doi:10.3897/pharmacia.69.e77076

Cited by 11 publications

(12 citation statements)

References 37 publications

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“…Untuk menentukan absorbansi supernatan pada panjang gelombang 260 dan 280nm, digunakan spektrofotometer UV-VIS. Pengukuran kandungan nitrogen asam nukleat dilakukan dengan panjang gelombang 260 nm, sedangkan pengukuran kandungan nitrogen protein sel dilakukan dengan panjang gelombang 280 nm (Nasri et al, 2021;Satria et al, 2022).…”

Section: Pengukuran Aktivitas Kebocoran Sel (Dna Dan Protein)unclassified

“…Setelah itu well plate dibersihkan dengan akuades, kemudian ditambahkan etanol 96%. Dimungkinkan untuk mengukur absorbansi atau optical density dengan menggunakan spektrofotometer UV/Vis yang ditetapkan pada Panjang gelombang 600 nm (Satria et al, 2022). Rumus yang digunakan untuk menghitung persentase penurunan pembentukan biofilm adalah sebagai berikut (1):…”

Section: Pengujian Antibiofilmunclassified

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Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi

Nasri,

Kaban,

Satria

et al. 2023

jharma

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Infectious diseases are conditions that can be brought on by a variety of different organisms, including bacteria, viruses, fungi, and parasites. Typhoid fever is an example of an infectious disease that frequently causes the body's defenses to come under attack. The fever associated with typhoid is a leading cause of death around the world. Infection with the bacterium Salmonella typhi is considered to be one of the potential causes of typhoid fever. When antibiotics are used to treat bacterial infections, there is a risk that the bacteria will become resistant to the medication. In day-to-day life, basil leaves are utilized both as a cooked vegetable and as a fresh vegetable. The antibacterial properties of kemangi leaves have yet to be fully uncovered. The purpose of this study was to investigate the various mechanisms that are involved in antibacterial activity. These mechanisms include cell leakage, minimum inhibitory concentration, minimum killing concentration, and antibiofilm activity. The minimum inhibitory concentration (MIC) was determined with the agar diffusion method, the minimum biofilm forming concentration (MBC) was determined with the staining method, and cell leakage was determined with UV-VIS spectrophotometry at wavelengths of 260 nm (DNA) and 280 nm (protein). Additionally, the formation of anti-biofilms was determined with microtiter plates and gentian violet staining. In the moderate category, the MIC value tests showed a concentration of 0.3125% with an inhibition zone value of 6.80 0.05 mm. These results were obtained from the MIC value. Because there was no detectable bacterial growth on the streaked medium, KBM was obtained at a concentration of 10%. At wavelengths of 260 and 280 nanometers, leakage cells show absorbance absorption. The percentage of biofilm activity that is measured when the concentration is increased to 25% is 44.82%. In conclusion, the ethanol extract of basil leaves possesses a possible antibacterial mechanism against Salmonella typhi. This was discovered through testing.

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Section: Pengukuran Aktivitas Kebocoran Sel (Dna Dan Protein)unclassified

Section: Pengujian Antibiofilmunclassified

Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi

Nasri,

Kaban,

Satria

et al. 2023

jharma

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“…Simplisia dari daun karenda direndam dengan etanol 96 % selama 5 hari, disaring residu diremaserasi dengan menggunakan pelarut etanol 96% selama 2 hari. Filtrate yang sudah ditampung dipekatkan hingga kental dengan menggunakan rotary evaporator (Satria et al, 2022)…”

Section: Pembuatan Ekstrakunclassified

“…Steroid antibakteri yang berhubungan dengan membran lipid, dan kepekaan terhadap komponen steroid yang bisa membuat kebocoran pada liposom. Steroid digunakan untuk mengobati infeksi pada bakteri (Satria et al, 2022). Steroid diketahui berinteraksi dengan membran sel fosfolipid, yang diketahui permeabel terhadap senyawa lipofilik.…”

Section: Tabel 3 Kategori Diameter Zona Hambat Bakteriunclassified

Uji Aktivitas Antibakteri Ekstrak Daun Karenda (Carissa carandas Linn.) Terhadap Bakteri Propionibacterium acne dan Staphylococcus epidermidis

Kaban,

Nasri,

Syahputra

et al. 2023

jharma

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Karenda leaves contain flavonoids, steroids or triterpenoids, saponins, and tannins that act as antimicrobial compounds. The aim of the study was to determine the antibacterial activity of the ethanol extract of Karenda leaves against several acne-causing bacteria. The research method involved characterizing the simplicia of karenda leaves and extract preparation by maceration using 96% ethanol as a solvent. and test the antibacterial activity of the ethanol extract of Karenda leaves using the agar diffusion method. The bacteria used were Propionibacterium acnes and Staphylococcus epidermis. The extract concentrations used were 300, 150, 125, 100, 75, 50, 25, and 5 mg/mL. Based on tests carried out by the ethanol extract of karenda leaves, the diameters of the inhibition zones were 7.2 ± 0.12 respectively; 7.8±0.10; 9.7±0.15; 11.23±0.25; 12.43±0.06; 13.57±0.06; 14.20±0.10; 16.47 ± 0.21 for Propionibacterium acne and 8.17 ± 0.06; 9.37 ± 0.15; 9.80 ± 0.10; 10.73 ± 0.15; 11.27±0.12; 12.43±0.06; 13.77±0.06; 15.47 ± 0.12 for Staphylococcus epidermis. he test results proved that 300 mg/mL was the best concentration for inhibiting the growth of Propionibacterium acnes and Staphylococcus epidermis bacteria.

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“…Numerous bioactivity features, such as antibacterial, antibiofilm, antioxidant, and anticancer activities, have been linked to the butterfly pea plant. For instance, Clitoria ternatea L. corolla extract was found to have antibiofilm efficacy against Streptococcus mutans ATCC 25175 and Staphylococcus aureus ATCC 6538 (Satria et al, 2022). Their research demonstrated that the extract significantly inhibited the growth of the test bacteria, S. aureus, and S. mutans by causing their cells to leak intramembrane fluid.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity of Clitoria ternatea Crude Extracts Against Pathogenic Bacteria

Zulkamal,

Zolhalim,

Aris

et al. 2023

MAB

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Clitoria ternatea, sometimes referred to as the Asian pigeon wings blue pea, the butterfly pea, or the Darwin pea, is a Fabaceae plant species that has been shown to possess antibacterial effects against several pathogenic microbes. Hence, the present study has been carried out to access the antibacterial activity of C. ternatea flower extracted with water and methanol against pathogenic bacteria. The well and disk diffusion assays were performed to determine the antibacterial activity of C. ternatea flower extracts. The efficacy of the extracts was then evaluated via broth microdilution assay to obtain MIC and MBC values and the growth reduction assay. Meanwhile, the DPPH scavenging test was used to assess the antioxidant activity of the crude extracts. The results of the well and disc diffusion assays showed that Gram-positive bacteria were more sensitive to both extracts compared to Gram-negative bacteria. Meanwhile, the methanolic extract showed higher antibacterial activity on both Gram-positive and Gram-negative bacteria compared to the aqueous extract. The results of the MIC and MBC tests showed that the methanolic extract was bactericidal to both Gram-positive and Gram-negative bacteria. The aqueous extract, however, demonstrated bacteriostatic activity against Gram-negative bacteria and bactericidal activity solely against Gram-positive bacteria. After a 24-h exposure period, a growth reduction assay showed that the methanolic extract could suppress both Gram-positive and Gram-negative bacteria by up to 99%. Meanwhile, the aqueous extract showed an inhibition percentage value ranging from 75% to 96% after an incubation period. The aqueous extract had the lowest antioxidant activity, with an EC50 value of 87.78 µg/mL, whereas the methanolic extract had a fair amount of antioxidant activity when compared to the control (quercetin), according to the DPPH scavenging assay. The present study suggests that C. ternatea extracts as a potential antibacterial agent against pathogenic bacteria with significant antioxidant activity and this activity may be due to the presence of anthocyanin and its derivatives.

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Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™

Cited by 11 publications

References 37 publications

Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi

Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi

Uji Aktivitas Antibakteri Ekstrak Daun Karenda (Carissa carandas Linn.) Terhadap Bakteri Propionibacterium acne dan Staphylococcus epidermidis

Bioactivity of Clitoria ternatea Crude Extracts Against Pathogenic Bacteria

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﻿Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™

Cited by 11 publications

References 37 publications

Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi

Mekanisme Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum basilicum L.) terhadap Salmonella typhi

Uji Aktivitas Antibakteri Ekstrak Daun Karenda (Carissa carandas Linn.) Terhadap Bakteri Propionibacterium acne dan Staphylococcus epidermidis

Bioactivity of Clitoria ternatea Crude Extracts Against Pathogenic Bacteria

Contact Info

Product

Resources

About

Antibacterial activity of Medan Butterfly pea (Clitoria ternatea L.) corolla extract against Streptococcus mutans ATCC®25175™ and Staphylococcus aureus ATCC®6538™