Antibacterial activity of lysozyme-binding proteins from chicken egg white

Shimazaki, Youji; Takahashi, Ayumi

doi:10.1016/j.mimet.2018.10.001

Cited by 20 publications

(16 citation statements)

References 25 publications

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“…Recent studies have demonstrated that components of the human secretions, belonging to innate immunity, are key elements of host defences acting as a pivotal barrier against viral injury [ 25 , 51 , 52 ]. Increasing interest has been very recently highlighted on the possible preventive role and adjunct treatment of lactoferrin [ 53 ], a glycoprotein of human secretions that is part of a non-specific defensive system, known to play an important role against microbial and viral infections and exerting anti-inflammatory effects on different mucosal surfaces and able to regulate iron metabolism [ 25 , 51 ].…”

Section: Lactoferrinmentioning

confidence: 99%

Lactoferrin as Protective Natural Barrier of Respiratory and Intestinal Mucosa against Coronavirus Infection and Inflammation

Campione

Cosio

Rosa

et al. 2020

IJMS

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Recently, the world has been dealing with a devastating global pandemic coronavirus infection, with more than 12 million infected worldwide and over 300,000 deaths as of May 15th 2020, related to a novel coronavirus (2019-nCoV), characterized by a spherical morphology and identified through next-generation sequencing. Although the respiratory tract is the primary portal of entry of SARS-CoV-2, gastrointestinal involvement associated with nausea, vomiting and diarrhoea may also occur. No drug or vaccine has been approved due to the absence of evidence deriving from rigorous clinical trials. Increasing interest has been highlighted on the possible preventative role and adjunct treatment of lactoferrin, glycoprotein of human secretions part of a non-specific defensive system, known to play a crucial role against microbial and viral infections and exerting anti-inflammatory effects on different mucosal surfaces and able to regulate iron metabolism. In this review, analysing lactoferrin properties, we propose designing a clinical trial to evaluate and verify its effect using a dual combination treatment with local, solubilized intranasal spray formulation and oral administration. Lactoferrin could counteract the coronavirus infection and inflammation, acting either as natural barrier of both respiratory and intestinal mucosa or reverting the iron disorders related to the viral colonization.

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Section: Lactoferrinmentioning

confidence: 99%

Lactoferrin as Protective Natural Barrier of Respiratory and Intestinal Mucosa against Coronavirus Infection and Inflammation

Campione

Cosio

Rosa

et al. 2020

IJMS

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“…Non-denaturing two-dimensional electrophoresis (2DE) was employed to separate lysozyme-binding ovotransferrin along with ovalbumin. After separating lysozyme-binding protein, ovotransferrin was extracted using isoelectric focusing (IEF) (Shimazaki and Takahashi, 2018). However, they were good for only laboratory use with no scale-up capability.…”

Section: Separation and Purification Of Ovotransferrinmentioning

confidence: 99%

“…Valenti et al (1982) reported that ovotransferrin immobilized on Sepharose 4B also had the iron-binding capacity and showed a bacteriostatic effect on E. coli. Shimazaki and Takahashi (2018) investigated the antibacterial activity of lysozyme-binding ovotransferrin separated with 2DE and IEF and found that it had bacteriolytic activity against B. subtilis and E. coli. Cooper et al (2019) reported that the overexpression of ovotransferrin in chicken embryonic cells improved their antimicrobial activity against Campylobacter jejuni.…”

Section: Antibacterial Activitymentioning

confidence: 99%

Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review

Rathnapala¹,

Ahn

Abeyrathne³

2021

Food Sci Biotechnol

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With emerging trends in the food and pharmaceutical industries, potential applications of egg-derived bioactive compounds were recognized. Ovotransferrin is a major egg white functional protein responsible for multiple bioactivities. The objectives of this review are to provide scientific evidence of the functional properties of chicken ovotransferrin and its derived peptides and to identify future research approaches and applications. Various easy, economical, and non-toxic methods have been reported to produce ovotransferrin with high yield and purity, and chemical and enzymatic approaches have been employed to release bioactive peptides. The native ovotransferrin is known to have antimicrobial, antioxidant, anticancer, and immunomodulatory activities. The peptides produced from ovotransferrin also are reported to have antioxidant, antimicrobial, antihypertensive, and anticancer properties. However, little or no application of these compounds in the food and pharmaceutical areas is available yet. Therefore, the practical application of OTF in nutraceutical and pharmaceutical areas are among the emerging areas of research.

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“…Thus, these methods cannot be used to separate and elute proteins by IEF in their native state. In contrast, we have developed a new method for separating protein complexes by using non‐denaturing 2‐DE, combined with non‐denaturing IEF and size separation, without using reagents like urea, thiourea, and detergents [14, 15]. When egg white proteins are separated by 2‐DE, several spots of 2‐DE exhibit lysozyme activity [15].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, we have developed a new method for separating protein complexes by using non‐denaturing 2‐DE, combined with non‐denaturing IEF and size separation, without using reagents like urea, thiourea, and detergents [14, 15]. When egg white proteins are separated by 2‐DE, several spots of 2‐DE exhibit lysozyme activity [15]. This activity is believed to be the origin of lysozyme‐binding proteins, such as lysozyme–ovotransferrin complex and lysozyme–ovalbumin complex that are separated by non‐denaturing 2‐DE.…”

Section: Introductionmentioning

confidence: 99%

Characterization of enzymatic activity of lysozyme in lysozyme–ovotransferrin complex before and after treatment with trypsin

Shimazaki

Yabu

2021

Separation Science Plus

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The native complex of lysozyme and ovotransferrin was isolated after the separation of egg white proteins using non‐denaturing isoelectric focusing, and mobilization toward the cathode by replacement of the cathodic sodium hydroxide solution with a phosphoric acid solution. The treatment of the lysozyme–ovotransferrin complex with trypsin significantly increased the enzymatic activity of lysozyme. Likewise, an increase in the enzymatic activity of lysozyme was also obtained when a mixture of the purified lysozyme and ovotransferrin was treated with trypsin. The increase in lysozyme enzymatic activity after tryptic treatment of the lysozyme–ovotransferrin complex resulted from the liberation of lysozyme from this complex. This was a consequence of the resistance of lysozyme to tryptic digestion and the digestion of ovotransferrin by trypsin into peptide fragments, which do not bind the lysozyme. The developed methodology could be used for the separation and isolation of other protein complexes and for testing their enzymatic and other activities.

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Antibacterial activity of lysozyme-binding proteins from chicken egg white

Cited by 20 publications

References 25 publications

Lactoferrin as Protective Natural Barrier of Respiratory and Intestinal Mucosa against Coronavirus Infection and Inflammation

Lactoferrin as Protective Natural Barrier of Respiratory and Intestinal Mucosa against Coronavirus Infection and Inflammation

Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review

Characterization of enzymatic activity of lysozyme in lysozyme–ovotransferrin complex before and after treatment with trypsin

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