Antibacterial activity of Limonium brasiliense (Baicuru) against multidrug-resistant bacteria using a statistical mixture design

Blainski, Andressa; Gionco, Bárbara; Oliveira, Admilton Gonçalves de; Andrade, Galdino; Scarmínio, Ieda Spacino; Silva, Denise B.; Lopes, Norberto P.; Mello, João Carlos Palazzo de

doi:10.1016/j.jep.2017.01.013

Cited by 17 publications

(20 citation statements)

References 42 publications

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“…To correlate the in vitro activities of the samples under study with their potential for investigation in future studies related to AD, principal component analysis (PCA) was applied to examine their distribution. Several authors have used multivariate statistical tools to rationalize the choice of potential extracts/fractions for investigation and have shown that this method is a suitable approach to check for similarities and differences and to create natural groupings among samples [22,30,37].…”

Section: Resultsmentioning

confidence: 99%

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In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

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Alzheimer’s disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia , Limonium brasiliense , Paullinia cupana , Poincianella pluviosa , Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ 25–35 toxicity in SH-SY5Y cells. The effect of the EAF of S . adstringens on mitochondrial membrane potential, lipid peroxidation, superoxide production and mRNA expression of 10 genes related to AD was also evaluated and the electropherogram fingerprints of EAFs were established by capillary electrophoresis. Chemometric tools were used to correlate the in vitro activities of the samples with their potential to be evaluated against AD and to divide extracts/fractions into four clusters. Pretreatment with the EAFs grouped in cluster 1 ( S . adstringens , P . pluviosa and L . brasiliense ) protected SH-SY5Y cells from Aβ 25-35 -induced toxicity. The EAF of S . adstringens at 15.62 μg/mL was able completely to inhibit the mitochondrial depolarization (69%), superoxide production (49%) and Aβ 25-35 -induced lipid peroxidation (35%). With respect to mRNA expression, the EAF of S . adstringens also prevented the MAPT mRNA overexpression (expression ratio of 2.387x) induced by Aβ 25–35 , which may be related to tau protein hyperphosphorylation. This is the first time that the neuroprotective effects of these fractions have been demonstrated and that the electropherogram fingerprints for the EAFs of G . ulmifolia , L . brasiliense , P . cupana , P . pluviosa and S . adstringens have been established. The study expands knowledge of the in vitro protective effects and quality control of the evaluated fractions.

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Section: Resultsmentioning

confidence: 99%

“…adstringens and T . catigua were prepared as previously described [22,23,25,27–29]. CEs and EAFs were concentrated and lyophilized before use.…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

et al. 2019

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“…Some species are cultivated as ornamental plants, whereas others have important medicinal properties [5,6]. Research on species of this genus has revealed important bioactivity concerning the free radical scavenging [7,8], antioxidant [5,[9][10][11], anti-inflammatory [10], antibacterial [12], antimicrobial [9], and antiviral [13] properties. e main phenolics identified as responsible for these activities were gallic acid, epigallocatechin gallate, and myricetin and isorhamnetin flavonoids [7,8,10,11,14].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Profile, Antioxidant Activity, and Enzyme Inhibitory Properties ofLimonium delicatulum(Girard) Kuntze andLimonium quesadenseErben

Ruiz-Riaguas

Zengin

Sinan

et al. 2020

Journal of Chemistry

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In this work, we report the phytochemical composition and bioactive potential of methanolic and aqueous extracts of leaves from Limonium delicatulum (Girard) Kuntze and Limonium quesadense Erben. The characterization and quantitation of individual phytochemicals were performed with liquid chromatography with diode array and electrospray-tandem mass spectrometry detection. Myricetin glycosides were abundant in L. delicatulum, whereas L. quesadense was rich in gallo(epi)catechin-O-gallate. Total phenolics, flavonols, and flavonoids were assayed with conventional methods. Antioxidant and radical scavenging assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating activity), as well as enzyme inhibitory assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase, glucosidase, and lipase), were performed to evaluate the potential bioactivity. The methanolic extracts of both species presented higher phenolic content and bioactivity than the aqueous extracts. Overall, L. quesadense extracts exhibited the most potent activity for most assays, representing a potential source of bioactive compounds for the pharmaceutical and food industries.

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“…The next stage would consist in evaluating interactions between selected combinations of compounds, notably through the use of statistical design, which consists of systematically investigating the effect of independent variables on a studied response. This technique was used to evaluate the influence of extraction solvent 28 or mixture composition 29 on the antimicrobial activity of plant extracts. Interactions between the four major constituents of Thymus vulgaris essential oil were also studied using statistical models to evaluate their insecticidal activity, highlighting synergy between thymol and p -cymene in a binary mixture 30 .…”

Section: Introductionmentioning

confidence: 99%

The antifungal potential of (Z)-ligustilide and the protective effect of eugenol demonstrated by a chemometric approach

Rodrigues

Éparvier

Odonne

et al. 2019

Sci Rep

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Mankind is on the verge of a postantibiotic era. New concepts are needed in our battle to attenuate infectious diseases around the world and broad spectrum plant-inspired synergistic pharmaceutical preparations should find their place in the global fight against pathogenic microorganisms. To progress towards the discovery of potent antifungal agents against human pathologies, we embarked upon developing chemometric approach coupled with statistical design to unravel the origin of the anticandidal potential of a set of 66 essential oils (EOs). EOs were analyzed by GC-MS and tested against Candida albicans and C . parapsilosis (Minimal Inhibitory Concentration, MIC). An Orthogonal Partial Least Square (OPLS) analysis allowed us to identify six molecules presumably responsible for the anticandidal activity of the oils: ( Z )-ligustilide, eugenol, eugenyl acetate, citral, thymol, and β-citronellol. These compounds were combined following a full factorial experimental design approach in order to optimize the anticandidal activity and selectivity index (SI = IC 50 (MRC 5 cells)/MIC) through reconstituted mixtures. ( Z )-Ligustilide and citral were the most active compounds, while ( Z )-ligustilide and eugenol were the two main factors that most contributed to the increase of the SI. These two terpenes can, therefore, be used to construct bioinspired synergistic anticandidal mixtures.

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Antibacterial activity of Limonium brasiliense (Baicuru) against multidrug-resistant bacteria using a statistical mixture design

Cited by 17 publications

References 42 publications

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

In vitro evaluation of the protective effects of plant extracts against amyloid-beta peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Phenolic Profile, Antioxidant Activity, and Enzyme Inhibitory Properties ofLimonium delicatulum(Girard) Kuntze andLimonium quesadenseErben

The antifungal potential of (Z)-ligustilide and the protective effect of eugenol demonstrated by a chemometric approach

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