Antibacterial activity of Jatropha tanjorensis leaf extracts against bacteria associated with wound infections from the clinical setting

BABAYEMI, OLAWALE OLADEJO; OKE, EBENEZER AYODELE; Bayode, Michael Tosin

doi:10.13057/nusbiosci/n130215

Cited by 2 publications

(2 citation statements)

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“…The earlier phytochemical screening revealed that phenols and polyphenols were strongly present in the C. aurea root extract in both the ethanol and methanol extracts 37 . In contrast to alkaloids and anthraquinone, saponin, tannin, flavonoid, steroid, and terpenoid were detected in the qualitative phytochemical analysis of Jatropha tanjorensis ethanol, methanol, and aqueous leaf extracts 38 . Another qualitative investigation found that Vernonia amygdalina leaf extracts in both aqueous and ethanol included tannins, saponins, alkaloids, oxalate, phylate, flavonoids, steroids, and phenols.…”

Section: Discussionmentioning

confidence: 82%

Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea

Wasihun¹,

Habteweld

Ayenew

2023

Sci Rep

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Local Ethiopians use Calpurnia aurea to treat skin infections. However, there is no adequate scientific confirmation. The aim of this study was to evaluate the antibacterial activities of the crude and the fractionated extracts of C. aurea leaves against different bacterial strains. The crude extract was made by maceration. The Soxhlet extraction method was used to obtain fractional extracts. The antibacterial activity against gram positive and gram negative American Type Culture Collection (ATCC) strains was performed using the agar diffusion technique. The minimum inhibitory concentration was determined through the microtiter broth dilution method. Preliminary phytochemical screening was done using standard techniques. The largest yield was obtained from ethanol fractional extract. Except for chloroform, which provided a relatively low yield when compared to petroleum ether, increasing the polarity of the extracting solvent improved the yield. The crude extract, solvent fractions, and the positive control showed inhibitory zone diameter, while the negative control did not. When used at a concentration of 75 mg/ml, the crude extract had similar antibacterial effects as gentamicin (0.1 mg/ml) and the ethanol fraction. The 2.5 mg/ml crude ethanol extract of C. aurea suppressed the growth of Pseudomonas aeruginosa, Streptococcus pneumoniae, and Staphylococcus aureus, according to the MIC values. The extract of C. aurea was more effective in inhibiting P. aeruginosa than the other gram-negative bacteria. Fractionation enhanced the antibacterial effect of the extract. All fractionated extracts showed the highest inhibition zone diameter against S. aureus. Petroleum ether extract had the greatest inhibition zone diameter against all bacterial strains. The non-polar components were more active compared to the more polar fractions. The phytochemical components discovered in the leaves of C. aurea included alkaloids, flavonoids, saponins, and tannins. Among these, the tannin content was remarkably high. The current results could provide a rational support for the traditional use of C. aurea to treat skin infections.

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Section: Discussionmentioning

confidence: 82%

Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea

Wasihun¹,

Habteweld

Ayenew

2023

Sci Rep

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“…PROBIOTICS' liquid culture filtrates [100ul] were pipetted into the wells. The plates were then incubated at 37°C for at least 24 h.; the diameters of inhibition zone around the well were measured [14,15] .…”

Section: Antibacterial Activity Of Probiotics' Metabolitesmentioning

confidence: 99%

Study of The Anti-bacterial Efficacy of Probiotics Culture Extracts on Extended Spectrum Beta Lactamase-Producing Multi-Drug Resistant Isolates, Bearing BLA-TEM-1 Gene, in Patients with Wound Infections in Hospitals

Shehab¹,

Shahat²,

Elhosiny³

et al. 2022

International Journal of Medical Arts

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Article informationBackground: Infections caused by Extended Spectrum Beta Lactamase-producing wound pathogens are difficult to be eradicated worldwide. The prevalence rates were reported as worldwide rising, Therefore, Imperative work is needed to detect safe, non-toxic and new-effective Therapy. The Aim of The Work:To detect the first gene was isolated from female patient called temonira-1 gene, bearing Extended Spectrum beta Lactamase-producing strains among multi-drug resistant bacteria isolated from patients with wound infections. And to evaluate the antibacterial effects of PROBIOTICS' culture extracts on these isolates.Patients and Methods: Overall, 320 patients selected from those with wound infections seen in Egypt, The Wound swab samples were collected, transported in brain heart infusion broth, isolated in pure culture and bacterial species were identified by the conventional microbiological methods. Antimicrobial susceptibility testing were done, followed by Phenotypic Detection of Extended Spectrum Beta Lactamase production using the double disk synergy test, PCR technique was done to detect isolates bearing the gene. The detected isolates had tested by the PROBIOTICS' culture extracts, using the well-agar diffusion method and the reducing colony count activity technique.Results: [48.54%] of the total multi-drug resistant pathogens identified were positive for ESβL by double disk synergy test. Among 100 ESβL-producers screened, 32 isolates were detected bearing TEM-1 gene that coding for these enzymes by using PCR technique. All of the studied ESβL producers, bearing TEM-1 gene were susceptible to PROBIOTICS' CULTURE EXTRACTS with inhibition zones diameter average inbetween 12 and 21 mm., in addition, [65.6% of strains] showing reducing colony count activity by 100 % [zero CFU]. Conclusion: PROBIOTICS' CULTURE EXTRACTS revealed excellent activity againstESβL-producing microorganisms that bearing TEM-1 gene. So, these extracts may be considered safe therapeutic promising options to treat such infections.

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Antibacterial activity of Jatropha tanjorensis leaf extracts against bacteria associated with wound infections from the clinical setting

Cited by 2 publications

References 14 publications

Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea

Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea

Study of The Anti-bacterial Efficacy of Probiotics Culture Extracts on Extended Spectrum Beta Lactamase-Producing Multi-Drug Resistant Isolates, Bearing BLA-TEM-1 Gene, in Patients with Wound Infections in Hospitals

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