Antibacterial Activity of <i>Leucaena leucocephala</i> Extracts on Growth of <i>Escherichia coli</i>

Rosida, Dedin Finatsiyatull; Djajati, Sri; Nilamayu, Z. A; Rosida,

doi:10.1166/asl.2017.10618

Cited by 4 publications

(7 citation statements)

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“…21,22 Different therapeutic and pharmacological properties of L. leucocephala (Lam.) de Wit have been reported; like antibacterial activity 23,24 . antioxidant and antidiabetic effects 25,26 stimulator for stimulates adipogenesis, lipolysis, and glucose uptake 26.…”

Section: Introductionmentioning

confidence: 92%

“…Successive extraction was carried out with nhexane, Chloroform and Methanol. 11 Before extraction with solvent of higher polarity marc was dried properly. Each concentrated extract was collected and stored separately.…”

Section: Extraction Preparation Of L Leucocephalamentioning

confidence: 99%

“…Additionally, it is one of the important medicinal plants from Mimosoideae's subfamily possessing anthelmintic, antimicrobial, antibacterial activity. 10,11 Natural chemical compounds with different pharmacological and therapeutic activity can be obtained from plants. Different parts of plants are widely used for the management of different significant and major illness.…”

Section: Introductionmentioning

confidence: 99%

“…Mimocine is a toxic amino acid present in higher amount in the leaves of the plant which reduce its activity as animal feed and reported to produce toxicity in animal and human. 28,29 Despite the widely reported 23,24,25,26 safe therapeutic and pharmaceutical activity of L. Leucocephala (Lam.) de Wit , a limited number of researches reported the in-vitro embryotoxicity and teratogenic effect of chloroform extract of leaf in zebrafish (Danio rerio) model are required before exploring further therapeutic potency.…”

Section: Introductionmentioning

confidence: 99%

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Embryotoxicity and Teratogenic Effect of Chloroform Extract of Leucaena leucocephala (Lam.) de Wit (Lam.) de Wit Leaf on Zebra fish (Danio Rerio)

Roy¹,

Lopamudra²,

Saha³

2020

Int J Pharma Bio Sci

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Leucaena leucocephala (Lam.) de Wit is a plant widely distributed in India and other Tropical countries widely as a source of nutrition to cattle and Humans. Literature review indicates varied medicinal property of L. leucocephala (Lam.) de Wit plant. However before further exploring its therapeutic potential, evaluation of in vitro embryotoxicity and teratogenic effect of the leaf extract of this plant are required to be performed in zebrafish model. The current study aimed to assess the toxicity of chloroform extract of L. leucocephala (Lam.) de Wit on Zebrafish (Danio Rerio). Successive soxhlation was carried out for extraction with solvent having variable polarity. Embryotoxicity and teratogenicity are studied by exposing twenty completely fertilized embryos to the extracts with varying concentrations. The zebrafish embryos were exposed to varying plant extract concentrations in a 0.1% DMSO solution, used as control. Toxicity , teratogenicity and some deformities in embryos was observed at a concentration of 600 µg/ml. Mortality was observed at higher concentration, whereas reduced hatching rate was observed with increasing concentration. Different physical deformities like kink tail, bent trunk, and enlarged yolk sac edema were attributed to the teratogenic effect of the extract observed at higher concentrations. Finally, LC50 value was determined to be significant with a p value < 0.0001. Teratogenic Index based on development of deformities was also significant (p value<0.0001) and claimed the nonteratogenic effect of the extract. The study divulges that plants with therapeutic efficacy could also develop some side effects when consumed at higher doses specially on the embryos. Detailed toxicity study should be carried out on medicinal plants to identify their safety and teratogenic effect on the embryos.

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Section: Introductionmentioning

confidence: 92%

Section: Extraction Preparation Of L Leucocephalamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Embryotoxicity and Teratogenic Effect of Chloroform Extract of Leucaena leucocephala (Lam.) de Wit (Lam.) de Wit Leaf on Zebra fish (Danio Rerio)

Roy¹,

Lopamudra²,

Saha³

2020

Int J Pharma Bio Sci

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“…It is also infamously known as the ‘miracle tree’ for its wide range of uses and benefits, including as ruminant feeds, for controlling soil erosion, and the production of timber and fuelwood [ 18 , 20 ]. Various studies have demonstrated several pharmacological activities exerted by this plant, including anti-bacterial [ 21 , 22 , 23 , 24 ], anti-inflammatory [ 25 , 26 ], anti-cancer [ 27 , 28 , 29 ], and anthelmintic activities [ 30 , 31 , 32 , 33 ], and it is believed to be due to the different types of phytochemicals present in the plant, such as alkaloids, flavonoids, tannins, and quercetin. However, the use of this plant as an animal feed could not be fully potentiated due to the presence of a toxic compound called mimosine that has been reported to cause alopecia, hair loss, growth retardation, and infertility in ruminants [ 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans

2022

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Helminth infections continue to be a neglected global threat in tropical regions, and there have been growing cases of anthelmintic resistance reported towards the existing anthelmintic drugs. Thus, the search for a novel anthelmintic agent has been increasing, especially those derived from plants. Leucaena leucocephala (LL) is a leguminous plant that is known to have several pharmacological activities, including anthelmintic activity. It is widely known to contain a toxic compound called mimosine, which we believed could be a potential lead candidate that could exert a potent anthelmintic effect. Hence, this study aimed to validate the presence of mimosine in LL extract and to investigate the anthelmintic effect of LL extract and mimosine on head thrashing, egg-laying, and pharyngeal pumping activities using the animal model Caenorhabditis elegans (C. elegans). Mimosine content in LL extract was confirmed through an HPLC analysis of spiking LL extract with different mimosine concentrations, whereby an increasing trend in peak heights was observed at a retention time of 0.9 min. LL extract and mimosine caused a significant dose-dependent increase in the percentage of worm mortality, which produced LC50s of 73 mg/mL and 6.39 mg/mL, respectively. Exposure of C. elegans to different concentrations of LL extract and mimosine significantly decreased the head thrashing, egg-laying, and mean pump amplitude of pharyngeal pumping activity. We speculated that these behavioral changes are due to the inhibitory effect of LL extract and mimosine on an L-type calcium channel called EGL-19. Our findings provide evidential support for the potential of LL extract and its active compound, mimosine, as novel anthelmintic candidates. However, the underlying mechanism of the anthelmintic action has yet to be elucidated.

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Making Hand Sanitizer With Spray Technique Through Utilization Of Betel Leaf Extract In Order To Improve A Clean And Healthy Living Culture

Latifah

2022

JPKK

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The Covid-19 virus is a virus that attacks the respiratory and causes death. A clean and healthy lifestyle is one of ways that can be done to survive during this pandemic. This community service program aims to make hand sanitizer with betel leaf extract. The target of this program is the community in Dusun Jetak, Kelurahan Wonorejo, Kecamatan Gondangrejo, Karanganyar. The method used to implement the service program is through the method of socialization and direct practice in making hand sanitizers. The result of the community service program is the ability of the community to make household-scale hand sanitizers independently. The results of the average score got by the community after the socialization program for making hand sanitizers are between 70-80. Each group in the hand sanitizer manufacturing program produces 2 liters. The level of public acceptance of hand sanitizer products with betel leaf extract is 85%. At the monitoring and evaluation stage, the community has an understanding level of 80-90%. Abstrak Virus Covid-19 merupakan virus yang menyerang pernafasan dan dapat menyebabkan kematian. Pola hidup bersih dan sehat merupakan salah satu usaha yang dapat dilakukan agar survive dalam masa pandemi ini. Program pengabdian masyarakat ini bertujuan untuk membuat hand sanitizer dengan ekstrak daun sirih. Sasaran pelaksanaan program ini adalah masyarakat Dusun Jetak, Kelurahan Wonorejo, Kecamatan Gondangrejo, Karanganyar. Metode yang dilakukan dalam pelaksanaan program pengabdian ini yaitu melalui metode sosialisasi dan praktik langsung dalam pembuatan hand sanitizer. Hasil dari program pengabdian masyarakat ini adalah masyarakat memiliki kemampuan dalam membuat mandiri hand sanitizer skala rumah tangga. Hasil rata-rata score yang diperoleh masyarakat pasca program sosialisasi pembuatan hand sanitizer berada dalam rentang nilai 70-80. Setiap kelompok dalam program pembuatan hand sanitizer ini menghasilkan 2 liter. Tingkat penerimaan masyarakat terhadap produk hand sanitizer dengan ekstrak daun sirih yaitu sebesar 85%. Pada tahap monitoring dan evaluasi masyarakat memiliki tingkat pemahaman sebesar 80-90%.

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Antibacterial Activity of Leucaena leucocephala Extracts on Growth of Escherichia coli

Cited by 4 publications

References 0 publications

Embryotoxicity and Teratogenic Effect of Chloroform Extract of Leucaena leucocephala (Lam.) de Wit (Lam.) de Wit Leaf on Zebra fish (Danio Rerio)

Embryotoxicity and Teratogenic Effect of Chloroform Extract of Leucaena leucocephala (Lam.) de Wit (Lam.) de Wit Leaf on Zebra fish (Danio Rerio)

Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans

Making Hand Sanitizer With Spray Technique Through Utilization Of Betel Leaf Extract In Order To Improve A Clean And Healthy Living Culture

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