Antibacterial activity of gutta-percha cones attributed to the zinc oxide component

Moorer, W.R.; Genet, J.M.

doi:10.1016/0030-4220(82)90468-6

Cited by 100 publications

(57 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Notably, it is seen that the unexposed controls were 87 ± 6% and 70 ± 13% viable for the uncoated and coated samples, respectively, with a visible but insignificant effect of the coating on viability (P > 0.05). From these data, we observe a slight inactivation consistent with the previously reported antibacterial properties of ZnO (see [18][19][20][21][22] and introduction section). A highly significant inactivation is apparent for the UV activated ZnO coating where only 30 ± 12% of the cells remains viable after 2 h UVA illumination compared to the 83 ± 4% viability observed for the uncoated but identically illuminated surface.…”

Section: Results Of Irradiationsupporting

confidence: 90%

“…The antibacterial properties of ZnO in powder or nanoparticle forms have also been studied [18][19][20][21][22] and can be further enhanced by ultraviolet light activation [23]. Nanostructured ZnO has interesting properties towards the photocatalytic decomposition of organic pollutants [24] and dyes [25], while La-doped and silane-coated ZnO nanoparticles have recently shown remarkable photocatalytic action against monocrotophos [26] and tetrachloroethylene [27], respectively.…”

Section: Homentioning

confidence: 99%

See 1 more Smart Citation

ZnO films grown by pulsed-laser deposition on soda lime glass substrates for the ultraviolet inactivation of Staphylococcus epidermidis biofilms

Mosnier

O’Haire

McGlynn

et al. 2009

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

We found that a ZnO film of 2 µm thickness which was laser-deposited at room temperature onto a plain soda lime glass substrate, exhibits notable antibacterial activity against a biofilm of Staphylococcus epidermidis when back-illuminated by a UVA light source with a peak emission wavelength of about 365 nm. X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), UV-visible absorption spectroscopy, Raman spectroscopy and x-ray photoemission spectroscopy (XPS) were used to characterize the ZnO films before and after the interactions with the biofilm and the ultraviolet light, respectively. The as-deposited film was highly textured with the wurtzite (0002) in-plane orientation (c-axis perpendicular to ZnO surface) and had a surface rms roughness of 49.7 nm. In the as-deposited film, the Zn to O ratio was 1 to 0.95. After the UV and biofilm treatments, the ZnO film surface had become rougher (rms roughness 68.1 nm) and presented uniform micron-sized pitting randomly distributed, while the zinc to oxygen ratio had become 1 to 2.2. In this case, both the UV-visible and Raman spectra pointed to degradation of the structural quality of the material. On the strength of these data, we propose a model for the mediation of the bactericidal activity in which the photogeneration of highly oxidizing species and the presence of active surface defect sites both play an important role. This study is of particular interest for the acute problem of disinfection of pathogenic biofilms which form on medical device/implant surfaces.

show abstract

Section: Results Of Irradiationsupporting

confidence: 90%

Section: Homentioning

confidence: 99%

ZnO films grown by pulsed-laser deposition on soda lime glass substrates for the ultraviolet inactivation of Staphylococcus epidermidis biofilms

Mosnier

O’Haire

McGlynn

et al. 2009

Science and Technology of Advanced Materials

View full text Add to dashboard Cite

show abstract

“…The antimicrobial action of the gpc, particularly attributed to the zinc oxide, can also contribute to decontaminate the radicular channel (25).…”

Section: Discussionmentioning

confidence: 99%

Disinfection of gutta-percha cones with chlorhexidine

Redmerski¹,

Bulla²,

Moreno³

et al. 2007

Braz. J. Microbiol.

View full text Add to dashboard Cite

This study investigated the effectiveness of detergent and aqueous solutions of 2% chlorhexidine digluconate in decontaminating gutta-percha cones (gpc) contaminated with bacteria, yeast, or bacterial spores. Guttapercha cones were contaminated with 10 7 -10 8 colony-forming units per milliliter (cfu/ml) of the following test organisms: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, or Candida albicans. Spores of Bacillus subtilis were also tested. Contaminated gpc were treated with the chlorhexidine solutions for 1, 5, 10, or 15 min. Each cone was then transferred to a tube containing saline and the micoorganisms were recovered after homogenization for cfu determination. Both detergent and aqueous chlorhexidine solutions were effective in eliminating S. aureus, E. faecalis, and C. albicans cells adhered on the surface of gpc within 1 min of exposure. E. coli was eliminated in 5 min with detergent solution. The Bacillus subtilis spores were eliminated by chlorhexidine solutions within 5 min. The results of this study demonstrated that both aqueous and detergent solutions of 2% chlorhexidine digluconate were effective in decontaminating gpc within 5 minutes of exposure.

show abstract

“…Zinc oxide (ZnO) is present in many root canal filling materials and has antimicrobial activity, although it is used mainly as a biocompatible, astringent chemical (11,12). ZnO generates reactive oxygen species that damage the microbial cell membrane, but its antimicrobial activity is limited (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial efficacy of gutta-percha supplemented with cetylpyridinium chloride

et al. 2016

View full text Add to dashboard Cite

To develop a root canal filling materialwith high antimicrobial activity, we prepared guttapercha supplemented with the cationic surfactant cetylpyridinium chloride (CPC). Thermoplastic gutta-percha was supplemented with 0.05%, 0.2%, or 0.8% CPC. The gutta-percha containing CPC was tightly packed at the bottom of a 24-well plate. Its antimicrobial activity against eight representative endodontic pathogens-including gram-positive and gram-negative bacteria and fungi-was evaluated by adding 0.5 mL of liquid samples containing pathogens to the wells. After 24 h of cultivation under appropriate conditions, microbial growth was analyzed by counting colony-forming units (CFU). Gutta-percha alone (without CPC) partially inhibited microbial growth, probably through the antimicrobial effect of some of its components, such as zinc oxide. Addition of CPC dose-dependently increased the antimicrobial efficacy of gutta-percha. Addition of 0.05%, 0.2%, and 0.8% CPC reduced the viable microbial number to below the lower limit of detection (20 CFU/mL) for all tested pathogens except Pseudomonas aeruginosa, which was detected in 0.8% CPC-containing guttapercha, although the viable number significantly decreased. Gutta-percha with CPC might be useful for preventing microbial infections during root canal therapy. (J Oral Sci 58, 277-282, 2016)

show abstract

Antibacterial activity of gutta-percha cones attributed to the zinc oxide component

Cited by 100 publications

References 31 publications

ZnO films grown by pulsed-laser deposition on soda lime glass substrates for the ultraviolet inactivation of Staphylococcus epidermidis biofilms

ZnO films grown by pulsed-laser deposition on soda lime glass substrates for the ultraviolet inactivation of Staphylococcus epidermidis biofilms

Disinfection of gutta-percha cones with chlorhexidine

Antimicrobial efficacy of gutta-percha supplemented with cetylpyridinium chloride

Contact Info

Product

Resources

About