Antibacterial activity of green gold and silver nanoparticles using ginger root extract

Abstract

BackgroundRecent studies demonstrated that the speed of synthesis, biocompatibility, and antimicrobial activity of gold (Au) and silver (Ag) metals is enhanced when biosynthesized in nano-sized particles. In the present study, Au NPs and Ag NPs were synthesized via a biological process using aqueous Ginger root extract and characterized by various spectroscopic methods. Methods & ResultsThe NPs were found to be in hexagonal and spherical shapes. The average particle size for Au and Ag NPs was found to be 20 nm and 15 nm, respectively. The dynamic light scattering (DLS) method has shown that the zeta potential values of synthesized NPs were found to be 5.7 mv and 7.11mv, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis of Ginger root extract revealed 25 compounds. The synthesized NPs showed signi cant activity against Staphylococcus aureus and Escherichia coli in vitro with IC50 and IC90 values for Au and Ag NPs, respectively, noted to be 7.5 and 7.3 µg/ml and 15 and 15.2 µg/ml for both bacterial strains. The protein leakage level was high and morphological changes occurred in bacteria treated with biosynthesized NPs. ConclusionThese results suggest that the biosynthesized metallic NPs show potential for application as antibacterial agents with enhanced activities.

Ginger (Zingiber officinale)-Mediated Green Synthesis of Silver-Doped Tin Oxide Nanoparticles and Evaluation of Its Antimicrobial Activity

“…These biomolecules present in the ginger root acted as the reducing, capping, and stabilizing agents for the synthesis of the nanoparticles. ,, During the synthesis of Ag-doped SnO 2 Nps the color changed from an orange color to a white precipitate after constant stirring for 30 min indicating the formation of Ag-doped SnO 2 as shown in Figure . Finally, the color of synthesized Ag-doped SnO 2 changed from white to dark gray after calcination at 400 °C for 4 h. The color change may be due to the plasmonic properties of silver nanoparticles . Moreover, the schematic representation for the synthesis of Ag-doped SnO 2 nanoparticles is shown in Figure .…”

“…Finally, the color of synthesized Ag-doped SnO 2 changed from white to dark gray after calcination at 400 °C for 4 h. The color change may be due to the plasmonic properties of silver nanoparticles. 52 Moreover, the schematic representation for the synthesis of Ag-doped SnO 2 nanoparticles is shown in Figure 2 .…”

Green Synthesis of Gold Nanoparticles by Aqueous Extract of Zingiber officinale: Characterization and Insight into Antimicrobial, Antioxidant, and In Vitro Cytotoxic Activities

“…In addition to helping treat and prevent arthritis, it has hepatoprotective and nephroprotective effects [33][34][35]. The propensity of ginger extract to facilitate the synthesis of metal nanoparticles was previously reported for copper [36], silver [37], and gold [38].…”

Tuning Biocompatibility and Bactericidal Efficacy as a Function of Doping of Gold in ZnO Nanocrystals

“…ZnO and Au NPs are extensively studied as promising antimicrobial agents, performing antibacterial activities, antifungal properties, , antiparasitic effects, , and antiviral potential. , They have diverse applications for human and animal health and even agriculture. From this perspective, these NPs have high biotechnological potential, stimulating investigations of their application in developing nanoproducts with biomedical applications. , …”

“…ZnO and Au NPs are extensively studied as promising antimicrobial agents, performing antibacterial activities, 5 antifungal properties, 6 , 7 antiparasitic effects, 8 , 9 and antiviral potential. 10 , 11 They have diverse applications for human and animal health and even agriculture.…”