Antibacterial Activity of Gentamicin-bonded Gelatin-sealed Polyethylene Terephthalate Vascular Prostheses

Ginalska, Grażyna; Osińska, Monika; Uryniak, Adam; Urbanik‐Sypniewska, Teresa; Belcarz, Anna; Rzeski, Wojciech; Wolski, Andrzej

doi:10.1016/j.ejvs.2004.12.030

Cited by 31 publications

(26 citation statements)

References 20 publications

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“…MIC, the lowest concentration of nanoparticles that inhibits growth of bacteria, was determined. Luria Bertani broth was used in the present study as it supports growth of S. aureus, E. coli, and P. aeruginosa (28). Cremophor EL (6% w/v) and Lauroglycol 90 (4% w/v) were treated similar to the formulations tested for MIC.…”

Section: Mic and Mbc Studiesmentioning

confidence: 99%

Silver Sulfadiazine Nanosystems for Burn Therapy

Venkataraman

Nagarsenker

2012

AAPS PharmSciTech

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Abstract. The objective of the present study was to formulate stable silver sulfadiazine (SSD) nanosuspensions and nanogels suitable for topical delivery with a view to increase bactericidal activity in burn therapy. SSD nanosuspensions were formulated using the microprecipitation-high-pressure homogenization technique. An optimized microsuspension of 0.5% SSD formulated with 6% Cremophor EL and 4% Lauroglycol 90 was subjected to 30 cycles of 1,000-bar pressure to give a nanosuspension with an average particle size of 367.85 nm. Transmission electron microscopy studies revealed that ovoid-to rectangularshaped SSD particles were present as clusters. It was evident through X-ray diffraction studies that SSD was present in amorphous state both in microprecipitate and in nanosuspension. SSD (0.5%) nanogels were prepared using 1% Carbopol 974 P for topical delivery of nanosized SSD. In vitro release studies demonstrated that SSD release was faster from solutions and nanosuspensions compared to gel formulation owing to the influence of the gel matrix on SSD release. The bacterial inhibitory efficiency of SSD nanosuspension was as good as that of SSD solution against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. In vivo studies revealed that a nanogel containing 0.5% SSD was more effective in wound healing compared to 0.5% and 1% marketed cream.

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Section: Mic and Mbc Studiesmentioning

confidence: 99%

Silver Sulfadiazine Nanosystems for Burn Therapy

Venkataraman

Nagarsenker

2012

AAPS PharmSciTech

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“…In this respect, aminoglycosides like gentamicin represent preferable antibiotics since they are effective against staphylococci and aerobic gram-negative bacilli and at the same time are considered to be safe for prophylactic therapy with a prolonged postantibiotic effect. Gentamicin has already proven its effectiveness in vascular surgery by protecting gelatine-sealed grafts [12] and in eradicating microbial adherence of staphylococcal isolates from vascular grafts [13].…”

Section: Discussionmentioning

confidence: 99%

Novel fatty acid gentamicin salts as slow-release drug carrier systems for anti-infective protection of vascular biomaterials

Obermeier

Matl

Schwabe

et al. 2012

J Mater Sci: Mater Med

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Infections of vascular prostheses are still a major risk in surgery. The current work presents an in vitro evaluation of novel slow release antibiotic coatings based on new gentamicin fatty acid salts for polytetrafluoroethylene grafts. These grafts were coated with gentamicin sodium dodecyl sulfate, gentamicin laurate and gentamicin palmitate. Drug release kinetics, anti-infective characteristics, biocompatibility and haemocompatibility of developed coatings were compared to commercially available gelatin sealed PTFE grafts (SEALPTFE™) and knitted silver coated Dacron(®) grafts (InterGard(®)). Each gentamicin fatty acid coating showed a continuous drug release in the first eight hours followed by a low continuous release. Grafts coated with gentamicin fatty acids reduced bacterial growth even beyond pathologically relevant high concentrations. Cytotoxicity levels depending on drug formulation bringing up gentamicin palmitate as the most promising biocompatible coating. Thrombelastography studies, ELISA assays and an amidolytic substrate assay confirmed haemocompatibility of developed gentamicin fatty acid coatings comparable to commercially available grafts.

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“…In time, there may be new methods of chemically binding antibiotics such as gentamicin to vascular prostheses covered with pig gelatine, which seems to be bacteriostatic to Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus strains [6]. Graft material specifically able to resist microbes by either changing their surface characteristics or by incorporating antimicrobial substances within their structure is being developed, and these endovascular devices may be useful in treating aorto-oesophageal fistula.…”

Section: Discussionmentioning

confidence: 99%

Secondary infection of a pre-existing thoracic aortic aneurysm by iatrogenic oesophageal perforation with aorta-oesophageal fistula formation

Chan

Ting

Law

et al. 2009

European Journal of Cardio-Thoracic Surgery

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We report a case of iatrogenic perforation of the oesophagus, with subsequent infection of a pre-existing thoracic aortic aneurysm and development of an aorto-oesophageal fistula resulting in life-threatening haemorrhage. This was successfully treated with endovascular aortic stent-graft using a Cook Zenith Tx2 device. Follow-up CT scan 3 months later showed that the aneurysm sac is getting smaller, and patient had no further septic or haemorrhagic complications. However, we emphasise that even if initial endovascular management of aorto-oesophageal fistula is successful, these patients often risk recurrent sepsis and therefore long-term clinical and radiological surveillance is mandatory.

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Antibacterial Activity of Gentamicin-bonded Gelatin-sealed Polyethylene Terephthalate Vascular Prostheses

Abstract: Covalent gentamicin immobilisation resulted in effective antibacterial protection of vascular prostheses against clinical and reference strains of S. aureus, E. coli and P. aeruginosa and allowed for a strong adherence of endothelial cells to antibiotic-modified prostheses.

Cited by 31 publications

References 20 publications

Silver Sulfadiazine Nanosystems for Burn Therapy

Silver Sulfadiazine Nanosystems for Burn Therapy

Novel fatty acid gentamicin salts as slow-release drug carrier systems for anti-infective protection of vascular biomaterials

Secondary infection of a pre-existing thoracic aortic aneurysm by iatrogenic oesophageal perforation with aorta-oesophageal fistula formation

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