Antibacterial activity of functional bioactive peptides derived from fish protein hydrolysate

Abstract

By-product removal in fish processing is estimated to be between 25 and 70% due to improper fish production handling and significant problems in the fish industry today. Therefore, one of the ways to manage the raw material of by-product is through protein hydrolysis. However, one of the most effective methods for managing this raw material, which includes skin, bones, heads, and viscera, is to convert their protein into peptides via hydrolysis methods, resulting in fish protein hydrolysate (FPH). FPH has been shown to have bioactive properties such as antibacterial, antihypertensive, antioxidative, anticancer, and anticoagulant properties. Bioactivity could be fully utilised in the future in both the nutraceutical and food industries. Numerous studies have been published on the acceptability of FPH in obtaining bioactive properties from various fish, particularly antibacterial activity. For example, the antibacterial peptide was identified as FPIGMGHGSRPA, consisting of 12 amino acids. Its antibacterial activity was tested against B. subtilis using 800 g/mL ampicillin. The inhibition zone increased with peptide concentration. This review discusses functional bioactive peptides derived from fish protein hydrolysate that can be used as antibacterial agents by inhibit Gram-positive and Gram-negative bacterial growth. It also covers fish species, parts, and hydrolysis methods to maximise yields.

Characterization of Acid- and Pepsin-Soluble Collagen Extracted from the Skin of Purple-Spotted Bigeye Snapper

“…To fix the situation, effective methods are needed to transform fish by-product wastes into important nutrients. A large percentage of by-products account for 50% to 70% of the original raw material, which contains collagen-rich heads, viscera, skin, bone, scales, and fins, depending on the processing method [ 9 , 10 ]. Moreover, fish skin has a high protein content, and collagens generated from fish skin have a wide range of applications as functional food components [ 2 ].…”

“…Moreover, fish skin has a high protein content, and collagens generated from fish skin have a wide range of applications as functional food components [ 2 ]. Furthermore, fish by-products also include a high amount of crude protein, ranging from 8 to 35%, and can be used as a source of collagen, gelatin, polyunsaturated fatty acids, enzymes, and essential amino acids [ 10 ]. Fish collagen has been used in the development of protein nutritional multivitamins, transporters, food additives, edible films, and coatings [ 2 ].…”

Statistical Optimization of Salmonella Survival in Different Protein Hydrolysates

“…The lack of survival of S. enterica at higher FPH concentrations can be attributed to an increase in the concentration of bioactive peptides, leading to an inhibitory effect on the test microorganisms. 39,40 A pattern similar to that of CH and SPH was observed with FPH being unable to impart cryoprotection to S. enterica at -18±3°C with no growth observed from Day 7 onwards.…”

Prospects of bioinformatics approach for exploring and mapping potential bioactive peptide of Rusip (The traditional Indonesian fermented anchovy): A Review

“…Several studies have reported the potential of bioactive peptides from fermented fish as antibacterial and anti-cholesterol agents in addition to their activity as an antioxidant and ACE inhibitor (Kusmarwati et al, 2014;Rinto et al, 2019). The FPIMGHGSRPA peptide sequences with 12 amino acids have been shown to have antibacterial action against Gram-positive and Gram-negative bacteria (Baco et al, 2022).…”