Antibacterial activity of ethanol extract of Beluntas leaves on Streptococcus mutans, Porphyromonas gingivalis, and Enterococcus faecalis

Sylvana, Dini; Amir, Masyhudi; Purnamasari, Cicih Bhakti; Iskandar, Abdillah; Asfirizal, Verry

doi:10.24198/pjd.vol33no3.19133

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…Sensitivity to incoming antibacterial compounds may be hindered due to the complex bacterial cell structure in P. gingivalis [46]. This is in line with previous research which shows that Grampositive bacteria have a larger inhibitory zone compared to Gram-negative bacteria [47]. This is due to the structure of E. faecalis in contrast to P. gingivalis.…”

Section: Figuresupporting

confidence: 85%

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Prasetya,

Fatimatuzzahro,

Ermawati

et al. 2024

Trends Sci

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Enterococcus faecalis is one of the bacteria that is commonly found in infected root canals, necrotic pulp and failure of root canal treatment, while Phorphyromonas gingivalis play a role in inducing periodontitis. Both bacteria can enter the blood circulation and lead to risk factors for systemic disease such as atherosclerosis. ChKM is the most widely used root canal sterilization, and also 0.2 % chlorhexidine mouthwash for long-term usage. It can destroy a potent cell and is allergenic. Developing an alternative material for root canal sterilization and mouthwash for plaque control is necessary. One of the alternative natural products that is predicted to have antibacterial effect is robusta coffee husk peels. This research aimed to investigate the antibacterial activity of robusta coffee (Coffea canephora) husk extract on E. faecalis and P. gingivalis. The robusta coffee husk was extracted using the maceration process with 96 % ethanol as the solvent. This research is an experimental laboratory, was tested by disk diffusion methods (Kirby-Baurer) using Gram-positive E. faecalis ATCC 29212 and Gram-negative P. gingivalis ATCC 33277 consisted of 6 groups: Aquadest as a negative control group, ChKM (for E.facecalis) and 0.2 % chlorhexidine (for P. gingivalis) as positive control, 4 treatment groups of coffee robusta husk extract with different concentrations there were 250, 250, 500, 750 and 1,000 mg/mL. The antibacterial effect was examined by measuring the clear zone around the disk paper. The results of this study proved the presence of antibacterial activity of coffee robusta husk extract against E. faecalis and P. gingivalis. The higher concentrations followed with a greater antibacterial effect. HIGHLIGHTS Robusta coffee husk extract (Coffea canephora) has an antibacterial property against Gram-positive and Gram-negative bacteria Robusta coffee husk was extracted using the maceration process, and disk diffusion methods (Kirby-Baurer) for antibacterial test Robusta coffee husk extract with a concentration of 1,000 mg/mL showed the greatest inhibition zone. The higher the concentration, the bigger inhibition zone appeared GRAPHICAL ABSTRACT

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Section: Figuresupporting

confidence: 85%

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Prasetya,

Fatimatuzzahro,

Ermawati

et al. 2024

Trends Sci

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“…The alkaloids can disrupt the amino acid structure of bacterial DNA, leading to bacterial lysis. [ 22 ] Saponins damage the cell membrane and cell wall permeability in the diffusion process, resulting in the release of enzymes, amino acids, nutrients, and water, leading to cell destabilization and cell death. [ 23 ] Tannins form a complex with protein in the cell wall, namely, proline, which can damage the cell wall.…”

Section: Discussionmentioning

confidence: 99%

“…[ 23 ] Tannins form a complex with protein in the cell wall, namely, proline, which can damage the cell wall. [ 22 ] The most abundant phenolic compound in M. indica L leaves is mangiferin. Mangiferin belongs to the xanthone C-glucosyl group, which can damage cell structure and cell membranes and inhibit bacterial protein synthesis.…”

Section: Discussionmentioning

confidence: 99%

The Antibacterial and Antibiofilm Effect of Amoxicillin and Mangifera indica L. Leaves Extract on Oral Pathogens

Soesanto,

Hepziba,

Yasnill

et al. 2023

Contemporary Clinical Dentistry

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Objective: This study aimed to determine the antibacterial and antibiofilm effects of amoxicillin combined with extract of Mangifera indica L. leaves against Staphylococcus aureus and Porphyromonas gingivalis. Materials and Methods: This was an experimental laboratory in vitro study with a posttest-only control group design. An antibacterial test using the plate count method and an antibiofilm test using the microtiter plate biofilm assay method were conducted. The research samples comprised extract of M. indica L. leaves with a concentration of 100%; amoxicillin and extract concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%; and amoxicillin. Dimethyl sulfoxide served as a negative control and co-amoxiclav served as a positive control. Results: The combination of amoxicillin and the extract exhibited an antibacterial effect against S. aureus at a concentration of 12.5% and higher and more effective than co-amoxiclac P. gingivalis at a concentration of 3.125% and higher. In the antibiofilm test, the combination of amoxicillin and the extract at a concentration of 25% after 1 h of incubation and a concentration of 6.25% after 3 h of incubation inhibited S. aureus. The inhibition of S. aureus biofilms at a concentration of 100% after 24 h of incubation was as effective as that of co-amoxiclav. The extract at a concentration of 25% over the entire incubation period showed more potent inhibition against the P. gingivalis biofilm than co-amoxiclav. Conclusions: The ethanolic extract of M. indica L. leaves and the combination of amoxicillin and the extract have the potential to inhibit the growth and formation of S. aureus and P. gingivalis biofilms.

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“…The leaf extract was evaluated for antibacterial potential (concentration 2.5 to 6.5%) towards E. faecalis, P. gingivalis, F. nucleatum, and S. mutans, which are accountable for root canal infections, periodontal disease, and caries. It showed significant growth inhibition towards E. faecalis (inhibition zone diameter (IZD) 12.6 mm), followed by S. mutans (IZD 12.2 mm) and P. gingivalis (IZD 12.2 mm) at a concentration of 6.5%, compared to chlorhexidine (IZDs 10.9, 11.4, and 10.6 mm, conc 2%) [126]. It also prohibited biofilm formation and decreased adhesion of E. faecalis and F. nucleatum in the micro-titter plate and auto-aggregation assays, respectively [127].…”

Section: Antimicrobial Activity and Pharmaceutical Preparationsmentioning

confidence: 99%

Phytoconstituents and Pharmacological Activities of Indian Camphorweed (Pluchea indica): A Multi-Potential Medicinal Plant of Nutritional and Ethnomedicinal Importance

et al. 2022

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Pluchea indica (L.) Less. (Asteraceae) commonly known as Indian camphorweed, pluchea, or marsh fleabane has gained great importance in various traditional medicines for its nutritional and medicinal benefits. It is utilized to cure several illnesses such as lumbago, kidney stones, leucorrhea, inflammation, gangrenous and atonic ulcer, hemorrhoids, dysentery, eye diseases, itchy skin, acid stomach, dysuria, abdominal pain, scabies, fever, sore muscles, dysentery, diabetes, rheumatism, etc. The plant or its leaves in the form of tea are commonly used for treating diabetes and rheumatism. The plant is a rich source of calcium, vitamin C, dietary fiber, and β-carotene. Various biomolecules have been isolated from P. indica, including thiophenes, terpenes, quinic acids, sterols, lignans, phenolics, and flavonoids. The current review reports detailed information about the phytoconstituents and pharmacological relevance of P. indica and the link to its traditional uses. The reported studies validated the efficacy and safety of P. indica, as well as supported its traditional uses for treating various ailments and promoting health and well-being. Thus, this could encourage the development of this plant into a healthy food supplement or medicine for the prevention and treatment of various diseases. However, further studies on the drug interactions, mechanism of action, pharmacokinetics, toxicology, and metabolism, as well as clinical trials, should be carried out.

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Antibacterial activity of ethanol extract of Beluntas leaves on Streptococcus mutans, Porphyromonas gingivalis, and Enterococcus faecalis

Cited by 4 publications

References 12 publications

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

Antibacterial Activity of Robusta Coffee (Coffea Canephora) Husk Extract Against Enterococcus Faecalis and Phorphyromonas Gingivalis: In Vitro Study

The Antibacterial and Antibiofilm Effect of Amoxicillin and Mangifera indica L. Leaves Extract on Oral Pathogens

Phytoconstituents and Pharmacological Activities of Indian Camphorweed (Pluchea indica): A Multi-Potential Medicinal Plant of Nutritional and Ethnomedicinal Importance

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