Antibacterial Activity of Endophytic Fungi Isolated from the Leaves of Jambu Biji (<i>Psidium guajava</i>L.)

Susilawati, Susilawati; Amalia, Ella; Oktariana, Desi; Khairunnisa, Maulia Sari

doi:10.1088/1742-6596/1095/1/012041

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…Thus, PCR followed by sequencing make it possible to compare the informative sequences contained in the ITS regions of an unknown fungal sample with the sequences identified and listed in a database [59]. This molecular method allowed the identification of the non-sporulating nine groups of endophytic fungi isolates of P. guajava leaves as Phomopsis sp., Xylaria longipes, Pallidocercospora thailandica, Xylaria grammica, Aspergillus versicolor, Cercospora apii, Phomopsis asparagi, Phyllosticta capitalensis, and Xylaria sp.. Our results differ from those obtained by Enyi et al [60] and Susilawati et al [61] who in Nigeria and Indonesia respectively isolated 2 and 4 endophytic fungi from the leaves of P. guajava. This difference shows that the colonization of plants by endophytic fungi varies from one locality to another.…”

Section: Discussioncontrasting

confidence: 99%

Diversity of Endophytic Fungi of Psidium guajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens

Tchamgoue

Fanche

Ndjakou

et al. 2020

JAMB

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Aims: The present study was carried out to determine the diversity of endophytic fungi that colonize the leaves of Psidium guajava, and to evaluate their antagonistic activity against Fusarium oxysporum f.sp. cubense and Mycosphaerella fijiensis which are the two main phytopathogens of banana plants. Place and Duration of Study: The research was carried out at Microbiology Laboratory, Faculty of Sciences, University of Yaoundé I and Microbiology Laboratory, Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine Bucharest, between April 2018 and February 2020. Methodology: Fragments of surface sterilized leaves of Psidium guajava were inoculated on Potato Dextrose Agar supplemented with chloramphenicol. The isolated and purified endophytic fungi were identified based on their macroscopic and microscopic characters using a mycological atlas as guide. The non-sporulating isolates were identified by comparing the ITS regions of their DNA to those of known fungi registered in the GenBank database. The antagonistic activity of the endophytic fungi isolated against Fusarium oxysporum and Mycosphaerella fijiensis was screened using dual culture method. Results: A total of 28 endophytic fungal were isolated from the leaves of Psidium guajava corresponding to a colonization frequency of 33.33%. These isolates were identified as: Aspergillus sp., Botryosphaeria sp., Fusarium sp., Neoscytalidium sp., Xylaria sp., Phyllosticta capitalensis, Cercospora apii, Xylaria longipes, Phomopsis sp., Phomopsis asparagi, Aspergillus versicolor, Pallidocercospora thailandica, and Xylaria grammica that belonged to the Deuteromycota and Ascomycota divisions. These endophytic fungi inhibited the growth of Fusarium oxysporum f.sp. cubense and Mycosphaerella fijiensis with the percentage inhibition varying respectively from 23.25% to 73.52% and from 21.36% to 100%. The species Botryosphaeria sp., Phomopsis sp., Phomopsis asparagi, and Xylaria longipes exhibited the greatest activity. Conclusion: The leaves of Psidium guajava have a fairly varied diversity of endophytic fungi. These endophytic fungi can serve as potential biological control agents against Panama and Sigatoka diseases of banana and also would produce secondary metabolites with antifungal properties.

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Section: Discussioncontrasting

confidence: 99%

Diversity of Endophytic Fungi of Psidium guajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens

Tchamgoue

Fanche

Ndjakou

et al. 2020

JAMB

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4a‐methyl‐dodecahydro‐1H-pyrrolo[3,4-b]quinoline-6‐one produced by Endophytic Fungi Aspergillus niger E12 obtained from Dodonaea viscosa Plant Leaves as a Novel Antibacterial Compound

Ramesh¹,

Kavitha²,

Rajalakshmi³

et al. 2022

Appl Biochem Biotechnol

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Antibacterial Potential of Endophytic Fungi Isolated from Psidium guajava (Guava) Leaf against Escherichia coli and Klebsiella pneumoniae

Hussaini, I. M.,

Ibrahim, S.,

Usman, A.

et al. 2024

UJMR

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Study’s Novelty/Excerpt This study investigates the antibacterial potential of endophytic fungi associated with Psidium guajava leaves against Escherichia coli and Klebsiella pneumoniae, revealing novel antimicrobial properties. Through isolation and identification of eight endophytic fungi, four were found to exhibit significant antibacterial activity. This work highlights the potential of endophytic fungi from P. guajava as a promising source of new antibacterial agents, contributing to the ongoing search for solutions to antimicrobial resistance. Full Abstract Antimicrobial resistance has been recognized as a major issue of public health concern and it remains a global threat of the health care system. Endophytic fungi associated with medicinal plants are reported as promising reservoir of novel antibiotics. The study aim was to determine the antibacterial potential of endophytic fungi associated with Psidium gaujava leaf against Escherichia coli and Klebsiella pneumoniae. Leaves of P. guajava were surface sterilized and inoculated on plates of Potato Dextrose Agar and incubated at room temperature. Endophytic fungal isolates that emerged were identified using their macroscopic (cultural) and microscopic characteristics. The endophytes were screen for antibacterial activity on E. coli and K. pneumoniae isolates. Antibacterial activity of endophytic fungi ethyl acetate extracts with antibacterial activity was also evaluated against isolates of E. coli and K. pneumoniae by agar well diffusion technique. Minimum Inhibitory Concentrations and Minimum Bactericidal Concentrations of the ethyl acetate extracts were also determined. Eight endophytic fungi were isolated from P. guajava leaves and four had antibacterial activity namely: Gonatobotrys sp. P21, Xylaria sp. P11, Fusarium sp. P22 and Trichoderma sp. P25. The extracts exhibited antibacterial activity with zones of inhibition ranging from 11.5 ± 0.5mm to 18.5 ± 1.5mm for E. coli and 12.5 ± 0.5mm to 21.0 ±1.0mm for K. pneumoniae. The MIC was found to be 500mg/mL and 250mg/mL for E. coli and K. pneumoniae respectively. Endophytic fungi isolated from P. gaujava leaf are potential source of novel antibacterial drug since they possess antibacterial activity against isolates of E. coli and K. pneumoniae.

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Antibacterial Activity of Endophytic Fungi Isolated from the Leaves of Jambu Biji (Psidium guajavaL.)

Cited by 3 publications

References 8 publications

Diversity of Endophytic Fungi of Psidium guajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens

Diversity of Endophytic Fungi of Psidium guajava (Myrtaceae) and Their Antagonistic Activity against Two Banana Pathogens

4a‐methyl‐dodecahydro‐1H-pyrrolo[3,4-b]quinoline-6‐one produced by Endophytic Fungi Aspergillus niger E12 obtained from Dodonaea viscosa Plant Leaves as a Novel Antibacterial Compound

Antibacterial Potential of Endophytic Fungi Isolated from Psidium guajava (Guava) Leaf against Escherichia coli and Klebsiella pneumoniae

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