Antibacterial activity of dental composites containing zinc oxide nanoparticles

Abstract

The resin-based dental composites commonly used in restorations result in more plaque accumulation than other materials. Bacterial biofilm growth contributes to secondary caries and failure of resinbased dental composites. Methods to inhibit biofilm growth on dental composites have been sought for several decades. It is demonstrated here that zinc oxide nanoparticles (ZnO-NPs) blended at 10% (w/w) fraction into dental composites display antimicrobial activity and reduce growth of bacterial biofilms by roughly 80% for a single-species model dental biofilm. Antibacterial effectiveness of ZnO-NPs was assessed against Streptococcus sobrinus ATCC 27352 grown both planktonically and as biofilms on composites. Direct contact inhibition was observed by scanning electron microscopy and confocal laser scanning microscopy while biofilm formation was quantified by viable counts. An 80% reduction in bacterial counts was observed with 10% ZnO-NP-containing composites compared with their unmodified counterpart, indicating a statistically significant suppression of biofilm growth. Although, 20% of the bacterial population survived and could form a biofilm layer again, 10% ZnO-NP-containing composites maintained at least some inhibitory activity even after the third generation of biofilm growth. Microscopy demonstrated continuous biofilm formation for unmodified composites after one day growth, but only sparsely distributed biofilms formed on 10% ZnO-NP-containing composites. The minimum inhibitory concentration of ZnO-NPs suspended in S. sobrinus planktonic culture was 50 μg/ml. 10% ZnO-NP-containing composites qualitatively showed less biofilm after one day anaerobic growth of a three-species initial colonizer biofilm after when compared to unmodified composites, but did not significantly reduce growth after three days.

The Study Effects of Dental Composite Resin as Antibacterial Agent Which Contain Nanoparticles of Zinc Oxide on the Bacteria Associated with Oral Infection

“…The most susceptible of bacterial isolates were Streptococcus mutans and Pseudomonas areuginosa with inhibition zones correspond to 15 mm, 17 mm in concentration 10% of synthesis ZnO/NPs dental composite in our study these results suggest that , the synthesis ZnO/NPs dental composite may play a vital role in the prevention of dental caries since Streptococcus mutans has been implicated in the initiation of caries 13mm, 15mm in concentration 7% of synthesis ZnO/NPs dental composite and 9 mm , 11 mm in concentration 5% of synthesis ZnO/NPs dental composite followed by other types of bacteria, the growth of Staphylococcus aureus and Klebsiella.spp were inhibited by synthesis ZnO/NPs dental composite with inhibition zones correspond to 10 mm, 13 mm; 11 mm, 11 mm, 5 mm, and 8 mm in concentrations of 10% , 7% and 5%wt respectively. Our results were consistent with Berdan Aydin Sevinc& Luke Hanley [23] results . In some studies , the technique of diffusion agar disc has been used for evaluation of antibacterial properties of cured composite resin [14,23], as in current study , used the disc-diffusion method , in which the bacterial isolates were inoculated to be tested by homogenous streaking (aseptically transfer inoculum) across the sterile agar plate by sterile wire loop , then incubated at 37ᴼC for 24 h. to read the inhibition results .…”

“…Our results were consistent with Berdan Aydin Sevinc& Luke Hanley [23] results . In some studies , the technique of diffusion agar disc has been used for evaluation of antibacterial properties of cured composite resin [14,23], as in current study , used the disc-diffusion method , in which the bacterial isolates were inoculated to be tested by homogenous streaking (aseptically transfer inoculum) across the sterile agar plate by sterile wire loop , then incubated at 37ᴼC for 24 h. to read the inhibition results . within this method, an inhibition growth zone around the material is produced mean the (+ve) results.…”

Effects of Filler Size on the Mechanical Properties of Polymer-filled Dental Composites: A Review of Recent Developments

“…[3][4][5] Excellent biocompatibility, superior aesthetic qualities as fillings, moderate cost compared with ceramics, and strong bonding ability to the tooth structure, make resin composite the preferred material in dental restorative applications. 1,6,7 Most dental composites consist of an organic matrix (polymer phase), inorganic fillers (dispersed phase) and interphase (coupling agent). 8 Usually, the organic matrix is based on methacrylates, epoxy and polyethylene.…”

Whole-cell Escherichia coli-based bio-sensor assay for dual zinc oxide nanoparticle toxicity mechanisms

“…For example these materials have well known antibacterial properties and have been incorporated into anti-bacterial fabrics [2] and orthopaedic cements [3][4][5] with efficacy against Gram negative and Gram positive organisms [6][7][8][9][10][11][12].…”