Antibacterial activity of crude extracts of cyanobacteria Phormidium and Microcoleus species

Thummajitsakul, Sirikul; Silprasit, Kun; Sittipraneed, Siriporn

doi:10.5897/ajmr12.152

Cited by 9 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and Microcoleus sp. at various concentrations (0.2, 0.06, 0.03 and 0.015 g mL −1 ) showed in vitro antibacterial activity against S. enteritidis and E. coli (Thummajitsakul, Silpprasit & Sittipraneed ), and a methanolic extract of Nostoc sp. showed antibacterial activity towards Gram‐positive Mycobacterium tuberculosis , S. aureus , and Gram‐negative S. typhi , P. aeruginosa , E. coli and Enterobacter aerogenes (Asthana, Tripathi, Srivastava, Singh, Singh, Nath, Srivastava & Srivastava ).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Vibrio spp. by 2‐Hydroxyethyl‐11‐hydroxyhexadec‐9‐enoate of Marine Cyanobacterium Leptolyngbya sp. LT 19

et al. 2016

View full text Add to dashboard Cite

Seven marine cyanobacteria were isolated from two regions of the Gulf of Thailand and evaluated by the agar diffusion method for antibacterial activity. Inhibitory compound was purified from the crude methanol extract and its structure was elucidated based on extensive spectroscopic analysis, including IR, 1D and 2D NMR spectra as well as mass spectrometry. A novel antimicrobial compound produced by the marine cyanobacterium Leptolyngbya sp. LT19 was identified to be a 2‐hydroxyethyl‐11‐hydroxyhexadec‐9‐enoate which has so far never been reported in microorganisms. Biological assays revealed that this novel compound exhibited antibacterial activities against the Gram‐negative, persistent shrimp pathogens, Vibrio harveyi and V. parahaemolyticus with minimal inhibitory concentration of 250–1000 and 350–1000 μg mL−1 respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of Vibrio spp. by 2‐Hydroxyethyl‐11‐hydroxyhexadec‐9‐enoate of Marine Cyanobacterium Leptolyngbya sp. LT 19

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Phormidium spp. have been previously reported to produce compounds that inhibit growth of different Gram-positive and Gram-negative bacterial strains, yeasts, and fungi [40][41][42]. Butanol extracts of Lyngbya sp.…”

Section: Cyanobacterial Morphotypesmentioning

confidence: 99%

Antibacterial and Antifungal Activities of Cyanobacterial Strains Isolated from Hot Springs in Oman

Sherwani

Abed

Dobretsov

et al. 2015

SQU Journal for Science

View full text Add to dashboard Cite

In this study, cyanobacterial microbial mats from five hot springs in Oman, namely Al Kasfah Rustaq, Al Thwara Nakhl, Al–Ali Hammam, Gala and Bowsher, were characterized using direct microscopy. Nine monoclonal cyanobacterial cultures were obtained and their extracts in butanol, dichloromethane (DCM) and hexane were screened for antibacterial and antifungal activities. Direct microscopy revealed the presence of 12 different unicellular and filamentous morphotypes, with different distribution in the various mats. Temperature seems to be one of the most important parameters that accounts for the differences in cyanobacterial composition of the mats. Cells of the nine isolates and their aqueous supernatants were subsequently extracted with butanol, DCM and hexane. Dried extracts were tested against nine bacterial (i.e. gram +ve Staphylococcus aureus, Bacillus subtilis and gram –ve, Escherichia coli, Klebsiella pneumoniae, Salmonella choleraesuis, S. enterica, Psuedomonas aeruginosa, Providencia stuartii, and Acinetobacter calcoaceticus) and two fungal pathogens (Rhizoctonia solani and Pythium sp.). All isolates exhibited antibacterial and antifungal activities, which depended mainly on the type of cyanobacterial culture, type of solvent used and the pathogen tested. The highest antibacterial activity was observed in Phormidium species, and butanol was found to be the most appropriate solvent to extract bioactivity from these cyanobacterial species. The results of this study suggest that thermal springs in Oman harbor diverse types of cyanobacteria, which may constitute an important source of antibacterial and antifungal compounds. Further investigation is needed to purify these compounds and find their chemical compositions and modes of action.

show abstract

“…Cyanobacterial extracts are known for their antibacterial action against a wide array of bacteria. Extracts of Phormidium species inhibited the growth of pathogenic strains of Pseudomonas aeruginosa , Staphylococcus aureus , Escherichia coli , and Streptococcus enteritidis [ 17 ]. Acetone extract of Oscillatoria agardhii caused growth inhibition of Salmonella senftenberg [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Laser Treatment Increases the Antimicrobial Efficacy of Cyanobacterial Extracts against Staphylococcusaureus (SA) and Methicillin-resistantStaphylococcus aureus (MRSA)

Naim

Semary

2022

IJERPH

View full text Add to dashboard Cite

Staphylococcus aureus (SA) and Methicillin-resistant Staphylococcus aureus (MRSA) are multidrug-resistant bacterial pathogens. A novel approach needs to be followed to combat these pathogens in an ecofriendly manner. Cyanobacterial extracts were previously proven to be affective as antimicrobial agents. To capitalize on this, laser treatments were used to increase their antimicrobial efficacy. Two cyanobacterial strains isolated from Al-Ahsa were identified using molecular methods. Their aqueous extracts were used in the antimicrobial bioassay for these two bacterial pathogens. The first group of aqueous extracts were exposed directly to laser treatment and used in antibacterial bioassay. In parallel, the cyanobacterial biomass of the two isolates was exposed to the laser, then aqueous extracts were prepared. The third group of extracts were not exposed to the laser and were used as a control. Time and distance were the factors tested as they affected the dose of the laser, both individually and in combination. In addition, accessory pigment estimation in extracts before and after laser exposure of extracts was also determined. The two cyanobacterial strains were identified as Thermoleptolyngbya sp. and Leptolyngbya sp. and the molecular analysis also confirmed the identity of pathogenic bacteria. The untreated cyanobacterial aqueous extracts had little effect against the two bacterial strains. In contrast, the extract directly exposed to the laser was significantly more effective, with an inhibition zone of 22.0 mm in the case of a time of 32 min and distance of 10 cm against S. aureus. Accessory pigment composition increased in extracts directly exposed to the laser. This is the first case report on the effect of lasers on enhancing the antimicrobial profile of cyanobacterial extracts against SA and MRSA bacterial pathogens, as well as enhancing accessory pigment content. The laser dose that was most effective was that of 32 min time and 10 cm distance of Thermoleptolyngbya sp. extract directly exposed to the laser, which highlights the importance of time for increasing the laser dose and consequently increasing its antimicrobial impact.

show abstract

Antibacterial activity of crude extracts of cyanobacteria Phormidium and Microcoleus species

Cited by 9 publications

References 24 publications

Inhibition of Vibrio spp. by 2‐Hydroxyethyl‐11‐hydroxyhexadec‐9‐enoate of Marine Cyanobacterium Leptolyngbya sp. LT 19

Inhibition of Vibrio spp. by 2‐Hydroxyethyl‐11‐hydroxyhexadec‐9‐enoate of Marine Cyanobacterium Leptolyngbya sp. LT 19

Antibacterial and Antifungal Activities of Cyanobacterial Strains Isolated from Hot Springs in Oman

Laser Treatment Increases the Antimicrobial Efficacy of Cyanobacterial Extracts against Staphylococcusaureus (SA) and Methicillin-resistantStaphylococcus aureus (MRSA)

Contact Info

Product

Resources

About