Antibacterial activity of components from <i>Lomatium californicum</i>

Chou, Shih‐Chien; Everngam, Molly C.; Sturtz, George; Beck, John J.

doi:10.1002/ptr.1828

Cited by 34 publications

(29 citation statements)

References 24 publications

(26 reference statements)

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“…Of these, falcarindiol (1) had the greatest antimicrobial activity and a strong additive not Gram-negative bacteria [24,[35][36][37]. It is possible that the activity in the fraction was potentiated by the co-isolated fatty acids, and these could have membrane permeabilising activity.…”

Section: Page 15 Of 35mentioning

confidence: 99%

Medicinal plant extracts with efflux inhibitory activity against Gram-negative bacteria

Garvey

Rahman

Gibbons

et al. 2011

International Journal of Antimicrobial Agents

108

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Section: Page 15 Of 35mentioning

confidence: 99%

Medicinal plant extracts with efflux inhibitory activity against Gram-negative bacteria

Garvey

Rahman

Gibbons

et al. 2011

International Journal of Antimicrobial Agents

108

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“…For example, coniferyl ferulate has antibacterial activity, antioxidant and anti-Alzheimer properties, and it was also shown to have a potential role in reversing multidrug resistance mechanisms acquired by tumor cells [42–44]. Feruloyl-CoA:monolignol transferase (AsFMT) from Chinese angelica ( Angelica sinensis ) is a BAHD enzyme involved in the synthesis of coniferyl ferulate by coupling feruloyl-CoA to the monolignol coniferyl alcohol [45].…”

Section: Resultsmentioning

confidence: 99%

Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

et al. 2016

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BackgroundBAHD acyltransferases, named after the first four biochemically characterized enzymes of the group, are plant-specific enzymes that catalyze the transfer of coenzyme A-activated donors onto various acceptor molecules. They are responsible for the synthesis in plants of a myriad of secondary metabolites, some of which are beneficial for humans either as therapeutics or as specialty chemicals such as flavors and fragrances. The production of pharmaceutical, nutraceutical and commodity chemicals using engineered microbes is an alternative, green route to energy-intensive chemical syntheses that consume petroleum-based precursors. However, identification of appropriate enzymes and validation of their functional expression in heterologous hosts is a prerequisite for the design and implementation of metabolic pathways in microbes for the synthesis of such target chemicals.ResultsFor the synthesis of valuable metabolites in the yeast Saccharomyces cerevisiae, we selected BAHD acyltransferases based on their preferred donor and acceptor substrates. In particular, BAHDs that use hydroxycinnamoyl-CoAs and/or benzoyl-CoA as donors were targeted because a large number of molecules beneficial to humans belong to this family of hydroxycinnamate and benzoate conjugates. The selected BAHD coding sequences were synthesized and cloned individually on a vector containing the Arabidopsis gene At4CL5, which encodes a promiscuous 4-coumarate:CoA ligase active on hydroxycinnamates and benzoates. The various S. cerevisiae strains obtained for co-expression of At4CL5 with the different BAHDs effectively produced a wide array of valuable hydroxycinnamate and benzoate conjugates upon addition of adequate combinations of donors and acceptor molecules. In particular, we report here for the first time the production in yeast of rosmarinic acid and its derivatives, quinate hydroxycinnamate esters such as chlorogenic acid, and glycerol hydroxycinnamate esters. Similarly, we achieved for the first time the microbial production of polyamine hydroxycinnamate amides; monolignol, malate and fatty alcohol hydroxycinnamate esters; tropane alkaloids; and benzoate/caffeate alcohol esters. In some instances, the additional expression of Flavobacterium johnsoniae tyrosine ammonia-lyase (FjTAL) allowed the synthesis of p-coumarate conjugates and eliminated the need to supplement the culture media with 4-hydroxycinnamate.ConclusionWe demonstrate in this study the effectiveness of expressing members of the plant BAHD acyltransferase family in yeast for the synthesis of numerous valuable hydroxycinnamate and benzoate conjugates.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0593-5) contains supplementary material, which is available to authorized users.

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“…Purification of the active ethyl acetate-soluble partition of a methanol extract of the bark of Haematoxylon brasiletto (Table 1) led to the isolation of eight known compounds: hematoxylin (1), brazilin (2) (Craig et al, 1965), caffeic acid (3) (Chou et al, 2006), methyl gallate (Kubo et al, 2004) (4), gallic acid (5) (Ani et al, 2006), phloroglucinol (6) (Yamaki et al, 1994), 4-hydroxycinnamic acid (7) (van der Watt and Pretorius, 2001;Souza et al, 2007), and 5-methoxypsoralen (8) (Manderfeld et al, 1997) (Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial compounds isolated from Haematoxylon brasiletto

Rivero-Cruz

2008

Journal of Ethnopharmacology

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Antibacterial activity of components from Lomatium californicum

Cited by 34 publications

References 24 publications

Medicinal plant extracts with efflux inhibitory activity against Gram-negative bacteria

Medicinal plant extracts with efflux inhibitory activity against Gram-negative bacteria

Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast

Antimicrobial compounds isolated from Haematoxylon brasiletto

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