Antibacterial activity of chitosan against Burkholderia pseudomallei

Abstract

The ability of Burkholderia pseudomallei to persist and survive in the environment is a health problem worldwide. Therefore, the antibacterial activities of chitosan against four environmental isolates of B. pseudomallei from soil in Khon Kaen, Thailand, were investigated. Antibacterial activities were assessed by a plate count technique after treatment with 0.2, 0.5, 1, 2 or 5 mg ml−1 chitosan for 0, 24 and 48 hr. Chitosan at 5 mg ml−1 completely killed all four B. pseudomallei isolates within 24 hr, whilst 2 mg ml−1 chitosan lowered the viability of B. pseudomallei by 20% within the same time span. Chitosan may act by disruption of the cell membrane, releasing intracellular components that can be detected spectrophotometrically at 260 and 280 nm. Transmission electron microscopy inspection of chitosan‐treated B. pseudomallei revealed damage to the bacterial membranes. This study demonstrated the effective antibacterial activity by chitosan against B. pseudomallei. Chitosan causes disruption of the bacterial cell membrane, release of intracellular constituents and cell death. This study revealed the inhibitory potential of chitosan for mitigating B. pseudomallei occurrences.

Isolation and characterization of chitosan from Ugandan edible mushrooms, Nile perch scales and banana weevils for biomedical applications

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Extracellular DNA facilitates bacterial adhesion during Burkholderia pseudomallei biofilm formation

“…Each bacterial isolate from glycerol stock at -80°C was grown on Ashdown’s agar and incubated at 37°C for 48 h. A single colony of B . pseudomallei was inoculated into 3 mL of Luria-Bertani (LB) broth containing appropriate antibiotics and incubated at 37°C with shaking (200 rpm) for 18 h. A 2% inoculum was added into 25 mL fresh LB broth and further incubated until OD 540 = 0.8–0.9 to provide bacterial starter culture [ 27 , 31 , 32 ].…”

Marine shell industrial wastes–an abundant source of chitin and its derivatives: constituents, pretreatment, fermentation, and pleiotropic applications-a revisit

“…The functional groups of chitosan and chitosan oligosaccharide, such as hydroxylamines and acetylated amine groups, react with various cell receptors that initiate interconnected pathway reactions in the living cells. It is particularly responsible for its beneficial properties, such as anti-inflammatory (Leal et al 2010;Santos-Moriano et al 2018), anti-carcinogenic (Huang et al 2006), anti-diabetic (Ju et al 2010), antimicrobial (Kamjumphol et al 2018;Tanha et al 2017), anti-HIV-I (Vo and Kim 2010), antioxidant (Ngo et al 2009), and anti-angiogenic (Quan et al 2009) activities.…”