Antibacterial activity of chemically defined chitosans: Influence of molecular weight, degree of acetylation and test organism

Mellegård, Hilde; Strand, Sabina P.; Christensen, Bjørn E.; Granum, Per Einar; Hardy, Simon P.

doi:10.1016/j.ijfoodmicro.2011.04.023

Cited by 133 publications

(95 citation statements)

References 45 publications

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“…This can result in increased microbial cell permeability, internal osmotic disequilibrium, and cell leakage (Helander et al, 2001;Rabea et al, 2003;Liu et al, 2004;Raafat et al, 2008;Mellegård et al, 2011). A 12-h exposure period to chitosan resulted in higher levels of glucose and protein in the supernatant of cell suspensions of Staphylococcus aureus than observed for the medium without chitosan.…”

Section: Effects Of Chitosan On Foodborne Pathogensmentioning

confidence: 99%

Shelf life extension of fresh fruit and vegetables by chitosan treatment

Romanazzi

Feliziani

Baños

et al. 2016

Critical Reviews in Food Science and Nutrition

263

143

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Among alternatives that are currently under investigation to replace the use of synthetic fungicides to control postharvest diseases in fresh produce and to extend their shelf life, chitosan application has shown promising disease control, at both preharvest and postharvest stages. Chitosan shows a dual mode of action, on the pathogen and on the plant, as it reduces the growth of decay-causing fungi and foodborne pathogens and induces resistance responses in the host tissues. Chitosan coating forms a semipermeable film on the surface of fruit and vegetables, thereby delaying the rate of respiration, decreasing weight loss, maintaining the overall quality, and prolonging the shelf life. Moreover, the coating can provide a substrate for incorporation of other functional food additives, such as minerals, vitamins, or other drugs or nutraceutical compounds that can be used to enhance the beneficial properties of fresh commodities, or in some cases the antimicrobial activity of chitosan. Chitosan coating has been approved as GRAS substance by USFDA, and its application is safe for the consumer and the environment. This review summarizes the most relevant and recent knowledge in the application of chitosan in postharvest disease control and maintenance of overall fruit and vegetable quality during postharvest storage.

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Section: Effects Of Chitosan On Foodborne Pathogensmentioning

confidence: 99%

Shelf life extension of fresh fruit and vegetables by chitosan treatment

Romanazzi

Feliziani

Baños

et al. 2016

Critical Reviews in Food Science and Nutrition

263

143

View full text Add to dashboard Cite

show abstract

“…Biological properties of chitosan make this polysaccharide an ideal component of calcium phosphate-based composites. Chitosan is a partially N-deacetylated derivative of chitin that naturally occurs in the exoskeleton of crustaceans [6,7]. This polysaccharide is often applied in tissue engineering because it biodegrades rapidly, is nontoxic, is prone to chemical and enzymatic modification, has similar structure to glycosaminoglycans (GAG) of bone extracellular matrix (ECM), and it stimulates cell adhesion, proliferation and osteoinduction [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Biological properties of novel chitosan-based composites for medical application as bone substitute

Przekora

Ginalska

2014

Open Life Sciences

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Hydroxyapatite is the main inorganic component of bones and teeth. In order to improve mechanical properties and surgical handiness of bioceramics, a plasticizing agent e.g. polysaccharide can be added. Chitosan is a polysaccharide with biological properties that make it an ideal component of bioceramics-based composites for medical application as bone substitute. In this study, biocompatibility of two types of novel krill chitosan-based composites was evaluated. In vitro experiments were carried out using human foetal osteoblast cell line. Cytotoxicity, cell adhesion, and bone ALP activity tests were performed to assess biocompatibility of the composites. Osteoblast growth on composites was observed using confocal microscope. Our results demonstrated that fabricated novel composites are non-toxic, are favorable to cell adhesion and growth, and provoke increase in b-ALP activity with time, thus inducing osteoblast differentiation. Based on this data composites have promising clinical potential as a bone defect filler in regenerative medicine. It is worth emphasizing that our work resulted in fabrication of flexible and surgical handy, bone substitutes that possess absolute biocompatibility with structural and mechanical properties similar to trabecular bone.

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“…Recent studies showed that antibacterial activity of chitosan and its derivatives partly depended on its molecular weight, with conflicting results [17,27,30,36,37]. In this study, we examined the effect of molecular weight on antibacterial activity.…”

Section: Bactericidal Activity Of Aemcs Derivativesmentioning

confidence: 96%

“…However, none of these studies performed sufficient characterization of the structure of the derivatives, making further structure-activity study not convincing. Further, many researches showed that molecular weight (Mw) of chitosan and its derivatives can affect their biological activities including antimicrobial and antioxidant activities [27][28][29][30]. To our best knowledge, the effect of Mw of aminoethyl modified chitosan derivatives (AEMCS) on antibacterial activities have not been elucidated.…”

Section: Introductionmentioning

confidence: 99%