Antibacterial Activity of Biosecur® Citrus Extract Surface Cleaner Against Vibrio Vulnificus

Abstract

This study evaluated the antibacterial activity of Biosecur® citrus extract surface cleaner against Vibrio vulnificus using plate count method. Two concentrations, 0.5% and 2% of Biosecur® surface cleaner were plated on Vibrio vulnificus Agar (VVA) and tested for reduction of Vibrio vulnificus. In order to investigate the lasting residual activity of Biosecur®, antibacterial activity tests were also performed at time intervals up to 2.5 h after Biosecur® was plated on VVA. Biosecur® showed 6-log reduction of Vibrio vulnificus at 2%, and 3-log reduction of Vibrio vulnificus at 0.5%. The antibacterial activity of 2% Biosecur® against Vibrio vulnificus was shown to be equivalent to that of tetracycline. The residual activity of 2% Biosecur® was shown to maintain for at least 2.5 h after application. This study confirmed the high activity and long lasting residual effect of a safe, non-toxic organic food grade surface cleaner.

Antimicrobial efficacy of alternative compounds for use in oral care toward biofilms from caries‐associated bacteria in vitro

“…From all compounds tested, only citrus extract 0.25% revealed a notable antimicrobial efficacy (>5 log 10 ) toward initial S. mutans biofilms similar to the positive controls CHX 0.2% and CPC 0.05%. This compound is a commercially available product comprising extracts from various citrus fruits (i.e., citrus reticulata , citrus aurantium amara, and citrus aurantium sinensis ), which has already been shown to have pronounced antimicrobial efficacy toward planktonic cultures of Vibrio vulnificus (Cormier, Scott, & Janes, ). In general, citrus fruits are known to be a rich source of phytochemicals, such as flavonoids and alkaloids, which show potent antimicrobial activity (Nogata et al., ; Oikeh, Omoregie, Oviasogie, & Oriakhi, ).…”

“…This compound is a commercially available product comprising extracts from various citrus fruits (i.e., citrus reticulata, citrus aurantium amara, and citrus aurantium sinensis), which has already been shown to have pronounced antimicrobial efficacy toward planktonic cultures of Vibrio vulnificus (Cormier, Scott, & Janes, 2013). In F I G U R E 3 Antimicrobial efficacy of selected compounds citrus extract, CHX, and CPC in polyspecies biofilms from Actinomyces naeslundii, Actinomyces odontolyticus, and S. mutans.…”

Efficacy of a New Commercial Ocular Spray Containing Oftasecur Citrus Extract for Reducing Microbial Load in the Conjunctiva of Patients Receiving Intravitreal Injections

“…In this context, citrus extract in liposomal form has been described as a natural, non-toxic, chemical-free antiseptic agent, which is composed of citrus bioflavonoids derived from some specific layers of citrus fruits; this natural compound was primarily developed in the food processing industry, because of its effective, broad-spectrum antimicrobial activity both against Gram-negative and some Gram-positive bacteria without the need of adopting nonorganic, synthetic preservatives [ 12 ].…”

In vitro Susceptibility and Evaluation of Techniques for Understanding the Mode of Action of a Promising Non-antibiotic Citrus Fruit Extract Against Several Pathogens

“…Of these plant extracts, citrus fruit extracts are showing an acceptable antibacterial activity against a broad range of Gram-negative and Gram-positive bacteria. Therefore, they are recommended as feed additives to improve animal health (Alvarez-Ordóñez et al, 2013), to control food-borne pathogens (Iturriaga et al, 2012; Vardaka et al, 2016; Tsiraki et al, 2018), against helminths (Abdelqader et al, 2012), as surface cleaners (Cormier et al, 2013) or even against insect pests on plants (Hollingsworth, 2005). BIOCITRO ® 1 is a commercialized citrus fruit extract obtained from organic cultures of grapefruit ( Citrus paradisi ), tangerine ( Citrus reticulata blanco ), bergamot ( Citrus aurantium ssp.…”