Antibacterial Activity of Bacteria Isolated from
 Phragmites Australis
 Against Multidrug-Resistant Human Pathogens

Delfino, Vania; Calonico, Carmela; Nostro, Antonella Lo; Castronovo, Lara Mitia; Duca, Sara Del; Chioccioli, Sofia; Coppini, Ester; Fibbi, Donatella; Vassallo, Alberto; Fani, Renato

doi:10.2217/fmb-2020-0244

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…ex Steud. have been reported [27][28][29]. In our pilot study involving the mass screening of more than 200 extracts from wild plants collected in Korea, we found that an extract of P. australis (Cav.)…”

Section: Introductionmentioning

confidence: 86%

Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites

Choi

Hwang

et al. 2022

Plants

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Acanthamoeba keratitis (AK) is an infectious ocular disease which is difficult to diagnose correctly and cure. Development of an effective and safe therapeutic drug for AK is needed. Our preliminary screening of more than 200 extracts from wild plants collected in Korea suggested the potential amoebicidal activity of Phragmites australis (Cav.) Trin. ex Steud. extract (PAE) against Acanthamoeba species. Here, we aimed to analyze the amoebicidal activity of PAE on Acanthamoeba and its underlying amoebicidal mechanism. PAE induced amoebicidal activity against both A. castellanii and A. polyphaga trophozoites, while it showed low cytotoxicity in human corneal epithelial cells (HCE-2) and human retinal pigment epithelial cells (ARPE-19). Transmission electron microscopy analysis showed subcellular morphological changes, such as increased granules, abnormal mitochondria, and atypical cyst wall formation, in the PAE-treated A. castellanii. Fluorometric apoptosis assay and TUNEL assay revealed apoptosis-like programmed cell death (PCD) in the PAE-treated A. castellanii. The PAE treatment increased reactive oxygen species production and reduced mitochondrial membrane potential in the amoeba. The enhanced expression of autophagy-associated genes was also detected. These results suggested that PAE exerted a promising amoebicidal effect on A. castellanii trophozoites via the PCD pathway. PAE could be a potential candidate for developing a therapeutic drug for AK.

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Section: Introductionmentioning

confidence: 86%

Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites

Choi

Hwang

et al. 2022

Plants

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“…The bacterial strains used as targets ( Table 1 ) in the cross-streaking test (see paragraph 2.8) are Bcc strains, grown on LB (Luria-Bertani) plates at 37 °C for 48 h, and a panel of 36 strains of Staphylococcus aureus , Staphylococcus haemolyticus , Staphylococcus epidermidis , P. aeruginosa , and K. pneumoniae , which were grown on TSA medium at 37 °C for 24 h. The resistance to antibiotics of these pathogenic strains has been previously characterized by the disk-diffusion method [18] . K. pneumoniae ATCC 700603, P. aeruginosa ATCC 27853, and S. aureus ATCC 25923 were obtained from Thermo Fisher Diagnostics S.p.A.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial activity of Arthrobacter strains isolated from Great Gobi A Strictly Protected Area, Mongolia

Bernacchi,

Semenzato,

di Mascolo

et al. 2024

AIMSMICRO

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<abstract> Desert soil hosts many microorganisms, whose activities are essential from an ecological viewpoint. Moreover, they are of great anthropic interest. The knowledge of extreme environments microbiomes may be beneficial for agriculture, technology, and human health. In this study, 11 <italic>Arthrobacter</italic> strains from topsoil samples collected from the Great Gobi A Strictly Protected Area in the Gobi Desert, were characterized by a combination of different techniques. The phylogenetic analysis, performed using their 16S rDNA sequences and the most similar <italic>Arthrobacter</italic> sequences found in databases, revealed that most of them were close to <italic>A. crystallopoietes</italic>, while others joined a sister group to the clade formed by <italic>A. humicola, A. pascens</italic>, and <italic>A. oryzae</italic>. The resistance of each strain to different antibiotics, heavy-metals, and NaCl was also tested as well as the inhibitory potential against human pathogens (i.e., <italic>Burkholderia</italic> ssp., <italic>Klebsiella pneumoniae, Pseudomonas aeruginosa</italic>, and <italic>Staphylococcus</italic> ssp.) via cross-streaking, to check the production of metabolites with antimicrobial activity. Data obtained revealed that all strains were resistant to heavy metals and were able to strongly interfere with the growth of many of the human pathogens tested. The volatile organic compounds (VOCs) profile of the 11 <italic>Arthrobacter</italic> strains was also analyzed. A total of 16 different metabolites were found, some of which were already known for having an inhibitory action against different Gram-positive and Gram-negative bacteria. Isolate MS-3A13, producing the highest quantity of VOCs, is the most efficient against <italic>Burkholderia cepacia</italic> complex (Bcc), <italic>K. pneumoniae</italic>, and coagulase-negative Staphylococci (CoNS) strains. This work highlights the importance of understanding microbial populations' phenotypical characteristics and dynamics in extreme environments to uncover the antimicrobial potential of new species and strains. </abstract>

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“…Arthrobacter sp. OVS8 was tested against 36 pathogenic strains: 10 CoNS, 10 P. aeruginosa, 10 S. aureus, and 6 K. pneumoniae strains, all characterized by their resistance to multiple antibiotics (as reported in Table 7) [65]. The strains were grown on TSA plates at 37 • C for 24 h.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Genomic, Molecular, and Phenotypic Characterization of Arthrobacter sp. OVS8, an Endophytic Bacterium Isolated from and Contributing to the Bioactive Compound Content of the Essential Oil of the Medicinal Plant Origanum vulgare L.

Semenzato

Duca

Vassallo

et al. 2023

IJMS

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Medicinal plants play an important role in the discovery of new bioactive compounds with antimicrobial activity, thanks to their pharmacological properties. However, members of their microbiota can also synthesize bioactive molecules. Among these, strains belonging to the genera Arthrobacter are commonly found associated with the plant’s microenvironments, showing plant growth-promoting (PGP) activity and bioremediation properties. However, their role as antimicrobial secondary metabolite producers has not been fully explored. The aim of this work was to characterize the Arthrobacter sp. OVS8 endophytic strain, isolated from the medicinal plant Origanum vulgare L., from molecular and phenotypic viewpoints to evaluate its adaptation and influence on the plant internal microenvironments and its potential as a producer of antibacterial volatile molecules (VOCs). Results obtained from the phenotypic and genomic characterization highlight its ability to produce volatile antimicrobials effective against multidrug-resistant (MDR) human pathogens and its putative PGP role as a producer of siderophores and degrader of organic and inorganic pollutants. The outcomes presented in this work identify Arthrobacter sp. OVS8 as an excellent starting point toward the exploitation of bacterial endophytes as antibiotics sources.

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Antibacterial Activity of Bacteria Isolated from Phragmites Australis Against Multidrug-Resistant Human Pathogens

Cited by 5 publications

References 31 publications

Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites

Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites

Antibacterial activity of <i>Arthrobacter</i> strains isolated from Great Gobi A Strictly Protected Area, Mongolia

Genomic, Molecular, and Phenotypic Characterization of Arthrobacter sp. OVS8, an Endophytic Bacterium Isolated from and Contributing to the Bioactive Compound Content of the Essential Oil of the Medicinal Plant Origanum vulgare L.

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