Antibacterial Activity of Bacillus amyloliquefaciens Phage Endolysin without Holin Conjugation.

Morita, Masatomo; Tanji, Yasunori; Mizoguchi, Katsunori; Soejima, Aya; Orito, Yuji; Unno, Hajime

doi:10.1263/jbb.91.469

Cited by 12 publications

(11 citation statements)

References 13 publications

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“…In addition to its high muralytic activity, OBPgp279 is suggested to intrinsically destabilize the OM in the Pseudomonas cell wall so that the endolysin reaches its enzymatic target, which could explain the observed reductions. A similar characteristic was suggested for the Bacillus amyloliquefaciens phage IAM1521 endolysin Lys1521 [38]. In the case of this endolysin, the presence of positively charged amino acids in the C-terminal cell wall binding part that interact with the OM and destabilize it, explained the antibacterial property of the endolysin [39].…”

Section: Discussionsupporting

confidence: 64%

Characterization of Modular Bacteriophage Endolysins from Myoviridae Phages OBP, 201φ2-1 and PVP-SE1

et al. 2012

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Peptidoglycan lytic enzymes (endolysins) induce bacterial host cell lysis in the late phase of the lytic bacteriophage replication cycle. Endolysins OBPgp279 (from Pseudomonas fluorescens phage OBP), PVP-SE1gp146 (Salmonella enterica serovar Enteritidis phage PVP-SE1) and 201ϕ2-1gp229 (Pseudomonas chlororaphis phage 201ϕ2-1) all possess a modular structure with an N-terminal cell wall binding domain and a C-terminal catalytic domain, a unique property for endolysins with a Gram-negative background. All three modular endolysins showed strong muralytic activity on the peptidoglycan of a broad range of Gram-negative bacteria, partly due to the presence of the cell wall binding domain. In the case of PVP-SE1gp146, this domain shows a binding affinity for Salmonella peptidoglycan that falls within the range of typical cell adhesion molecules (Kaff = 1.26×106 M−1). Remarkably, PVP-SE1gp146 turns out to be thermoresistant up to temperatures of 90°C, making it a potential candidate as antibacterial component in hurdle technology for food preservation. OBPgp279, on the other hand, is suggested to intrinsically destabilize the outer membrane of Pseudomonas species, thereby gaining access to their peptidoglycan and exerts an antibacterial activity of 1 logarithmic unit reduction. Addition of 0.5 mM EDTA significantly increases the antibacterial activity of the three modular endolysins up to 2–3 logarithmic units reduction. This research work offers perspectives towards elucidation of the structural differences explaining the unique biochemical and antibacterial properties of OBPgp279, PVP-SE1gp146 and 201ϕ2-1gp229. Furthermore, these endolysins extensively enlarge the pool of potential antibacterial compounds used against multi-drug resistant Gram-negative bacterial infections.

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Section: Discussionsupporting

confidence: 64%

Characterization of Modular Bacteriophage Endolysins from Myoviridae Phages OBP, 201φ2-1 and PVP-SE1

et al. 2012

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“…In the previous study, the antibacterial spectrum of endolysin was analyzed [2]. Since endolysin showed strong antibacterial action against P. aeruginosa PAO1, a Gram‐negative bacterium, P. aeruginosa PAO1 was used for the antibacterial assay in this study (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Release of the progeny phage, which is considered to be the last phenomenon of bacteriophage infection, is usually carried out by two proteins: a murein‐degrading enzyme termed endolysin, essential in hydrolyzing the peptidoglycan, and a tunnel‐like protein termed holin, which produces non‐specific holes in the cytoplasmic membrane to facilitate the transport of endolysin to the periplasm. Previously, the antibacterial activity of Bacillus amyloliquefaciens (IAM1521) phage endolysin was characterized [1,2]. Generally, peptidoglycan‐degrading enzymes are active against Gram‐positive bacteria, but are limited in activity against Gram‐negative bacteria, which have an outer membrane, a permeability barrier that inhibits the access of the enzymes to the peptidoglycan layer.…”

Section: Introductionmentioning

confidence: 99%

Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram‐negative bacteria

et al. 2001

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To analyze the antibacterial activity of Bacillus amyloliquefaciens phage endolysin, nine deletion derivatives of the endolysin were constructed. Each deletion mutant was overexpressed, purified and characterized. The catalytic domain was located on the N-terminal region and the C-terminus had an affinity with the bacterial envelope. The enzymatic activity remained in spite of the deletion of the C-terminal 116-amino acid region; however, the antibacterial activity was lost. These results indicate that antibacterial action requires both the C-terminal cell-binding and the N-terminal enzymatic activities. ß

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“…yoeB mutants display an increased rate of autolysis in response to nutrient depletion and various cell envelope stress conditions. The process of autolysis in B. subtilis 168 can be aided by mutating yoeB gene and in the process making it independent of xylose regulation (168).…”

Section: Methods For Extractionmentioning

confidence: 99%

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

Muhammadi

Shabina

Afzal

et al. 2015

Green Chemistry Letters and Reviews

266

139

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Polyhydroxyalkanoates (PHAs) are intracellular aliphatic polyesters synthesized as energy reserves, in the form of water-insoluble, nano-sized discrete and optically dense granules in cytoplasm by a diverse bacteria and some archae under conditions of limiting nutrients in the presence of excess carbon source. Bacteria synthesize different PHAs from coenzyme A thioesters of respective hydroxyalkanoic acid, and degrade intracellularly for reuse and extracellularly in natural environments by other microorganisms. In vivo, PHAs exist as amorphous mobile liquid and water-insoluble inclusions but in vitro, exhibit material and mechanical properties, ranging from stiff and brittle crystalline to elastomeric and molding, similar to petrochemical thermoplastics. Further, they are hydrophobic, isotactic, biocompatible and exhibit piezoelectric properties. But as commodity plastics their applications are limited by high production cost, low yield, in vivo degradation, complexity of technology and difficulty of extraction. Therefore, to replace the conventional plastic with PHAs, it is prerequisite to standardize the PHA production systems.

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Antibacterial Activity of Bacillus amyloliquefaciens Phage Endolysin without Holin Conjugation.

Cited by 12 publications

References 13 publications

Characterization of Modular Bacteriophage Endolysins from Myoviridae Phages OBP, 201φ2-1 and PVP-SE1

Characterization of Modular Bacteriophage Endolysins from Myoviridae Phages OBP, 201φ2-1 and PVP-SE1

Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin against Gram‐negative bacteria

Bacterial polyhydroxyalkanoates-eco-friendly next generation plastic: Production, biocompatibility, biodegradation, physical properties and applications

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