Antibacterial activity of Aspergillus isolated from different Algerian ecosystems

Abstract

Thirty two strains of Aspergillus genus were isolated from soil samples obtained from particular ecosystems: Laghouat endowed with a desert climate and Teleghma with a warm and temperate climate. Based on the morphological aspect, this collection was subdivided into ten phenotypic groups. This identification was confirmed by molecular analyzes using a molecular marker of the genu ribosomal 18s. This marker will allow us to associate our sequences with those of known organisms. In order to discover new antibiotic molecules, the antibacterial activity was performed against two Gram positive bacteria: Staphylococcus aureus and Bacillus subtilis and also two Gram-negative bacteria: Escherichia coli and Pseudomonas aeroginosa, using two different techniques: Agar cylinders and disks technique. The results show that the fungal species have an activity against at least one test bacterium. The Gram positive bacteria were the most affected, where the averages of the inhibition zones reach 34.33 mm. However, Gram-negative bacteria showed less important results from 0 to 12.00 mm. It is recorded that the antibacterial activity was studied for the first time in the following two species: Aspergillus niveus and Aspergillus wentii. Furthermore, an indepth study is underway on bioguided fractionation, which would identify individual components and lead to the isolation of the active ingredient.

Antibacterial Activity of the Metabolites of Aspergillus Chevalieri and Trichoderma Harzianum

“…The antibiotics produced are components of the metabolites and are capable of inhibiting the growth of other microorganisms [11]. Aspergillic acid, penicillic acid and fumagillin [17] are well-known examples of such antibiotics. The metabolites showed significant activity against P. aeruginosa which was comparable to ciprofloxacin, the reference antibiotic.…”

“…In humans, A. chevalieri is an opportunistic pathogen which causes skin infections [16]. Aspergillus species on the other hand, are ubiquitous molds [17], being the most prevalent fungi present in all soils. Many species in this genus are characterized as opportunistic avirulent plant symbionts [18].…”

Extraction and preliminary study of antibacterial compounds of three species of Aspergillus genus

“…Three strains of Aspergillus genus were used to evaluate their antibacterial activity: Aspergillus quadrilineatus (MH109538) isolated from the soil taken from an arid zone; Laghouat (located at 400 km south of Algiers), Aspergillus niveus (MH109544), and Aspergillus wentii (MH109545) isolated from a thermal soil in the region of Teleghma (located in north-eastern Algeria). It should be noted that isolation and identification steps of fungal strains have been detailed in another article (Bramki et al, 2017).…”