Antibacterial Activity of As-Annealed TiO2Nanotubes Doped with Ag Nanoparticles against Periodontal Pathogens

Yeniyol, Sinem; He, Zhiming; Yüksel, Behiye; Boylan, Robert; Ürgen, Mustafa; Özdemir, Tayfun; Ricci, John L.

doi:10.1155/2014/829496

Cited by 24 publications

(17 citation statements)

References 47 publications

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“…21, 22, 23, 24, 25, 26, 27, 28, 29, 30 In addition, dental composites containing nanoparticles of silver (NAg) have been developed to achieve antibacterial activities. 31, 32, 33, 34, 35 Incorporating NAg with a high surface area into the resin is desirable to reduce the Ag filler level required for antibacterial efficacy. This approach enables a high antibacterial activity at a low Ag filler level, without compromising the composite colour and mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms

et al. 2016

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Dental composites are commonly used restorative materials; however, secondary caries due to biofilm acids remains a major problem. The objectives of this study were (1) to develop a composite containing quaternary ammonium dimethacrylate (QADM), nanoparticles of silver (NAg), and nanoparticles of amorphous calcium phosphate (NACP), and (2) to conduct the first investigation of the mechanical properties, biofilm response and acid production vs water-ageing time from 1 day to 12 months. A 4 × 5 design was utilized, with four composites (NACP-QADM composite, NACP-NAg composite, NACP-QADM-NAg composite, and a commercial control composite), and five water-ageing time periods (1 day, and 3, 6, 9, and 12 months). After each water-ageing period, the mechanical properties of the resins were measured in a three-point flexure, and antibacterial properties were tested via a dental plaque biofilm model using human saliva as an inoculum. After 12 months of water-ageing, NACP-QADM-NAg had a flexural strength and elastic modulus matching those of the commercial control (P>0.1). Incorporation of QADM or NAg into the NACP composite greatly reduced biofilm viability, metabolic activity and acid production. A composite containing both QADM and NAg possessed a stronger antibacterial capability than one with QADM or NAg alone (P<0.05). The anti-biofilm activity was maintained after 12 months of water-ageing and showed no significant decrease with increasing time (P>0.1). In conclusion, the NACP-QADM-NAg composite decreased biofilm viability and lactic acid production, while matching the load-bearing capability of a commercial composite. There was no decrease in its antibacterial properties after 1 year of water-ageing. The durable antibacterial and mechanical properties indicate that NACP-QADM-NAg composites may be useful in dental restorations to combat caries.

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Section: Introductionmentioning

confidence: 99%

One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms

et al. 2016

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“…However, the synthesis of Ag NPs onto TiO 2 nanotubes requires careful consideration, especially when a clinical application is considered. [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

“…Silver nanoparticles can be synthesised using a variety of techniques such as electrochemical method [20], thermal decomposition [18], and laser ablation [22]. However, chemical reduction is one of the simplest method of fabricating Ag NPs on to the surface of TiO 2 nanotubes, whereby a reducing agent is used to reduce the silver ions to Ag NPs [23,24].…”

Section: Introductionmentioning

confidence: 99%

Anodised TiO2 nanotubes as a scaffold for antibacterial silver nanoparticles on titanium implants

Gunputh

Handy

et al. 2018

Materials Science and Engineering: C

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Medical grade titanium alloy is widely used for bone/dental implants, but the material alone has no innate antimicrobial properties that would reduce infection risk following surgery. However, silver nanoparticles (Ag NPs) are known to be antibacterial. This study investigated the growth of Ag NPs on titanium dioxide nanotubes (TiO NTs) on Ti-6Al-4V discs. The TiO NTs were grown on the Ti alloy using an electrochemical method, and then decorated with Ag NPs. The Ag NPs were synthesised by chemical reduction using δ-gluconolactone. A silver ammonia solution (silver nitrate + liquid ammonia) was used as the source of silver. Two separate approaches were used: (1) The δ-gluconolactone was mixed with the silver ammonia and then exposed to the TiO NTs (the 'mixing method'), which produced micron-sized clusters of the Ag NPs. (2) The TiO NTs were exposed to the silver ammonia first and then to δ-gluconolactone (the 'sequential addition method'), which resulted in the formation of nano-sized clusters of the nanoparticles. The Ag-TiO composites were confirmed by scanning electron microscopy and the elements analysed using energy dispersive X-ray spectroscopy (EDS). The composite coatings were exposed to a simulated body fluid for 24 h in order to determine the total Ag released. The release from the micron-sized clusters from the mixing method (14.6 ± 0.67 ppm) was higher than that from the nano-sized clusters (4.05 ± 0.36 ppm) when 0.015 M of silver ammonia was used. Additionally, Staphylococcus aureus, was cultured on the composite coatings for 24 h. Both the micron- and nano-sized clusters of the Ag NPs were found to be antibacterial using the Live/Dead assay. Overall, δ-gluconolactone was successfully used to reduce silver to Ag NPs on the surface of TiO NTs. The sequential addition method was the preferred method of synthesis because of its slower silver release, better coverage of the Ag-NPs on the TiO NTs and strong antibacterial properties.

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“…Titania nanotubes can provide abundant space to improve osteogenic activity and can act as carriers for drug delivery. [17][18][19] A previous study showed that nanotubes can exhibit moderate antibacterial ability. 17 Tetracycline (TET), a broad-spectrum antibiotic, can inhibit gram-negative and some gram-positive pathogens.…”

Section: Introductionmentioning

confidence: 99%

Decreased Porphyromonas gingivalis adhesion and improved biocompatibility on tetracycline-loaded TiO2 nanotubes: an in vitro study

Sun¹,

Xu²,

Sun³

et al. 2018

IJN

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BackgroundTitanium dioxide (TiO2) nanotubes are often used as carriers for loading materials such as drugs, proteins, and growth factors.Materials and methodsIn this study, we loaded tetracycline onto TiO2 nanotubes to demonstrate its antibacterial properties and biocompatibility. The two-layered anodic TiO2 nanotubes with a honeycomb-like porous structure were fabricated by using a two-step anodization, and they were loaded with tetracycline by using a simplified lyophilization method and vacuum drying. Their physical properties, such as chemical compositions, wettability, and surface morphologies of the different samples, were observed and measured by X-ray photoelectron spectroscopy (XPS), contact angle measurement, and scanning electron microscopy (SEM). The in vitro growth behaviors of mouse bone marrow stromal cells (BMSCs) on these substrates were investigated.ResultsThe TiO2 nanotube (NT) substrates and the tetracycline-loaded TiO2 nanotube (NT-T) substrates revealed a crucial potential for promoting the adhesion, proliferation, and differentiation of BMSCs. Similarly, the NT-T substrates displayed a sudden release of tetracycline in the first 15 minutes of their administration, and the release tended to be stable 90 minutes later. The antibacterial performances of the prepared substrates were assessed with Porphyromonas gingivalis. The result showed that NT and NT-T substrates had antibacterial capacities.ConclusionOverall, this research provides a promising method with potential for clinical translation by allowing local slow release of antimicrobial compounds by loading them onto constructed nanotubes.

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Antibacterial Activity of As-Annealed TiO₂Nanotubes Doped with Ag Nanoparticles against Periodontal Pathogens

Cited by 24 publications

References 47 publications

One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms

One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms

Anodised TiO2 nanotubes as a scaffold for antibacterial silver nanoparticles on titanium implants

Decreased <em>Porphyromonas gingivalis</em> adhesion and improved biocompatibility on tetracycline-loaded TiO<sub>2</sub> nanotubes: an in vitro study

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Antibacterial Activity of As-Annealed TiO2Nanotubes Doped with Ag Nanoparticles against Periodontal Pathogens

Cited by 24 publications

References 47 publications

One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms

One-year water-ageing of calcium phosphate composite containing nano-silver and quaternary ammonium to inhibit biofilms

Anodised TiO2 nanotubes as a scaffold for antibacterial silver nanoparticles on titanium implants

Decreased <em>Porphyromonas gingivalis</em> adhesion and improved biocompatibility on tetracycline-loaded TiO<sub>2</sub>&nbsp;nanotubes: an in vitro study

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Antibacterial Activity of As-Annealed TiO₂Nanotubes Doped with Ag Nanoparticles against Periodontal Pathogens

Decreased <em>Porphyromonas gingivalis</em> adhesion and improved biocompatibility on tetracycline-loaded TiO<sub>2</sub> nanotubes: an in vitro study