Antibacterial activity of antimicrobial peptide gcDefb1 against foodborne pathogenic bacteria and its application in pork storage

Dong, Bin; Lin, Yanjun; Wang, Jun; Du, Wei; Sun, Chunlong; Fu, Shijun; Wu, Tao

doi:10.1007/s10068-022-01060-9

Cited by 9 publications

(1 citation statement)

References 30 publications

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“…Bacteriocins with antilisterial activity have been isolated from diverse lactic acid bacteria (LAB) such as Enterococcus spp., Lactobacillus spp., Leuconostoc spp., and Weissella spp. (Dong et al, 2022;Ghalfi et al, 2006;Todorov, 2010;Woo et al, 2021;Yang and Moon, 2021). Among them, nisin, an antibiotic bacteriocin produced by Lactococcus lactis, is the only bacteriocin used in the food industry in many countries to improve food safety and extend the shelf life of foods (Churklam et al, 2020;van Kuijk et al, 2012).…”

Section: Articlementioning

confidence: 99%

Characterization of Novel Amylase-Sensitive, Anti-Listerial Class IId Bacteriocin, Agilicin C7 Produced by Ligilactobacillus agilis C7

Yoo¹,

Song²,

Vasquez³

et al. 2023

Food Sci Anim Resour

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Among various biological agents, bacteriocins are important candidates to control Listeria monocytogenes which is a foodborne pathogen. In this study, a novel bacteriocin, named agilicin C7, was isolated from Ligilactobacillus agilis C7 showing inhibitory activity against L. monocytogenes. Agilicin C7 biosynthesis gene was characterized by bioinformatics analyses and heterologously expressed in Escherichia coli for further study. The anti-listeria activity of recombinant agilicin C7 (r-agilicin C7) was lost by proteases and α-amylase, suggesting that agilicin C7 is a glycoprotein. r-Agilicin C7 has wide pH and thermal stability and is also stable in various organic solvents. It destroyed L. monocytogenes by damaging the integrity of the cell envelope. These properties of r-agilicin C7 indicate that agilicin C7 is a novel amylase-sensitive anti-listerial Class IId bacteriocin. Physicochemical stability and inhibitory activity against L. monocytogenes of r-agilicin C7 suggest that it can be applied to control L. monocytogenes in the food industry, including dairy and meat products.

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