“…LBD B showed higher antimicrobial activity than LBD A, C-D , LBD X and LBD Y , and the highest activity (< 2.5 μM) from LBD B was observed for the gram-negative bacterium E. coli, Shigella flexnerib, Aeromonas hydrophilac and the gram-positive bacteria B. subtilis and C. glutamicum. Again, in our previous study( 36 ), 40 μM MIC was chosen as a threshold, and any values higher than that are considered not active; thus, no further experiments regarding bioassays were continued. Overall, the antibacterial spectrum of LBD A-D was broadened compared to that of LBD Mj , and more interestingly, LBD A-D did not develop any resistance to all strains tested in this study, regardless of whether they were tested at either sub-MIC or sur-MIC levels, at least under our laboratory conditions.…”