Antibacterial activity of a pepsin‐derived bovine hemoglobin fragment

Froidevaux, Rénato; Krier, François; Nedjar‐Arroume, Naïma; Vercaigne-Marko, Dominique; Kosciarz, Elisabeth; Ruckebusch, Cyril; Dhulster, Pascal; Guillochon, Didier

doi:10.1016/s0014-5793(01)02171-8

Cited by 104 publications

(69 citation statements)

References 38 publications

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“…Although the exact mechanism of microbial inhibition by HB is not known, it has been noted in earlier works [63,64]. The iron may be directly protecting proteins by blocking active sites recognized by enzymes of degradation (supported by the increase in antibody signal after treatment with iron chelator), or it may be providing protection indirectly by binding to oxygen, and thus preventing oxidative damage [68,69] or outcompeting bacterial mechanisms, similar to ferritins [45].…”

Section: Discussionmentioning

confidence: 99%

A role for iron and oxygen chemistry in preserving soft tissues, cells and molecules from deep time

Schweitzer¹,

et al. 2014

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The persistence of original soft tissues in Mesozoic fossil bone is not explained by current chemical degradation models. We identified iron particles (goethite-aFeO(OH)) associated with soft tissues recovered from two Mesozoic dinosaurs, using transmission electron microscopy, electron energy loss spectroscopy, micro-X-ray diffraction and Fe micro-X-ray absorption nearedge structure. Iron chelators increased fossil tissue immunoreactivity to multiple antibodies dramatically, suggesting a role for iron in both preserving and masking proteins in fossil tissues. Haemoglobin (HB) increased tissue stability more than 200-fold, from approximately 3 days to more than two years at room temperature (258C) in an ostrich blood vessel model developed to test post-mortem 'tissue fixation' by cross-linking or peroxidation. HB-induced solution hypoxia coupled with iron chelation enhances preservation as follows: HB þ O 2 . HB 2 O 2 . 2O 2 þO 2 . The well-known O 2 /haeme interactions in the chemistry of life, such as respiration and bioenergetics, are complemented by O 2 /haeme interactions in the preservation of fossil soft tissues.

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Section: Discussionmentioning

confidence: 99%

A role for iron and oxygen chemistry in preserving soft tissues, cells and molecules from deep time

Schweitzer¹,

et al. 2014

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“…2.3 Heavy metal resistance and antibiotic tolerance of isolate strains 2.3.1 Heavy metal resistance A MIC (minimal inhibitory concentration) is the lowest concentration of the heavy metals that completely inhibited strain growth (Froidevaux et al, 2001). MIC of cadmium -: 0-10% positive; +: 90%-100% positive; N: not identify.…”

Section: Isolation and Identification Of Strainsmentioning

confidence: 99%

Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE

Dou

et al. 2007

Journal of Environmental Sciences

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This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing. We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing, China, by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain, and found two dominant strains in the DGGE profile. Using special culture media, we isolated two strong cadmium-resistant bacterial strains. On the basis of morphological, physiological, and biochemical characteristics, BIOLOG, and 16S rDNA sequencing, the two strains were identified as Bacillus cereus and Enterobacter cloacae. Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined. E. cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B. cereus.

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“…Antibacterial activity towards the latter two organisms is of particular importance, as both organisms are frequently implicated in food-borne illnesses. Froidevaux and others [130] have also demonstrated the anti-bacterial activity of pepsin-digested bovine hemoglobin. The antibacterial peptide in this case was the 1-23 fragment (VLSAADKGNVKAAWGKVGGHAAE) of the alpha chain of bovine hemoglobin and it exhibited antibacterial activity towards Micrococcus luteus, which is a bacterial strain commonly used as a sensitive strain for the detection of antibiotic substances.…”

Section: Bioactive Compoundsmentioning

confidence: 90%