Antibacterial activity of a new lectin isolated from the marine sponge Chondrilla caribensis

Abstract

A new lectin from the marine sponge Chondrilla caribensis (CCL) was isolated by affinity chromatography in Sepharose 6B media. CCL is a homotetrameric protein formed by subunits of 15,445 ±2Da. The lectin showed affinity for disaccharides containing galactose and mucin. Mass spectrometric analysis revealed about 50% of amino acid sequence of CCL, which showed similarity with a lectin isolated from Aplysina lactuca. Secondary structure consisted of 10% α-helix, 74% β-sheet/β-turn and 16% coil, and this profile was unaltered in a broad range of pH and temperatures. CCL agglutinated Staphylococcus aureus, S epidermidis and Escherichia coli, and it was able to reduce biofilm biomass, but showed no inhibition of planktonic growth of these bacteria. CCL activity was inhibited by α-lactose, indicating that Carbohydrate Recognition Domain (CRD) of the lectin was involved in antibiofilm activity.

CAPTURE OF LECTINS FROM JACKFRUIT (Artocarpus integrifolia) SEEDS IN A SINGLE STEP USING A SUPERMACROPOROUS ION EXCHANGE CRYOGEL

“…Lectin purification from sea sponge was investigated by Marques et al (2018), who used affinity chromatography to obtain a 21-fold purification and 34% recovery. Sharma et al (2017) purified lectins from Dioscorea bulbifera by affinity techniques, also obtaining a 21-fold purification but a 16.8% recovery.…”

Marine Biocompounds for Neuroprotection—A Review

“…They fulfill different biological roles, such as pathogen recognition and opsonization, complement activation, immune function regulation, or they act as antifreeze proteins or prevent polyspermy during fertilization [155]. So far, lectins have been isolated from a variety of marine animals, such as sponges (Aplysina lactuca, Cliona varians, Suberites domuncula, Axinella corrugata, and Chondrilla caribensis), annelids (Cinachyrella apion, Chaetopterus variopedatus and Serpula vermicularis), mollusks (Aplysia dactylomela, Mytilus galloprovincialis, Argopecten irradians, Ruditapes philippinarum, Madiolus modiolus, and Crenomytilus grayanus), arthropods (Tachypleus tridentatus and Penaeus monodon), sea cucumbers (Holothuria scabra, Holothuria grisea, and Cucumaria echinata), ascidians (Didemnum ternatanum), amphioxus (Branchiostoma belcheri and Branchiostoma japonicum), and fish (Aristichtys nobilis, Silurus asotus, Oncorhynchus tshawytscha, Epinephelus coioides, Oncorhynchus mykiss, Rachycentron canadum, and Paralichthys olivaceus) [157,158]. As they have the ability to modulate molecular targets in the central nervous system, lectins might be involved in processes associated with neuroplasticity, neurobehavioral effects, and neuroprotection [159].…”

Karakterisasi Biokimia Lektin Makroalga Sargassum polycystum dan Turbinaria ornata/Biochemical Characterisation of Lectin Derived from Sargassum polycystum and Turbinaria ornata Macroalgae

“…Sebagai contoh, lektin dapat digunakan untuk mendeteksi metastasis sel kanker (Wolters-Eisfeld & Schumacher, 2017), mendeteksi sel yang mengalami apoptosis (Seco-Rovira, Beltran-Frutos, Hernandez-Martinez, Ferrer, & Pastor, 2017) atau untuk pengenalan patogen (Brooks, 2017). Selain potensi pemanfaatannya di bidang imunologi dan glikobiologi, lektin yang diisolasi dari berbagai sumber juga dilaporkan sebagai antibakteri (Marques et al, 2018;Vasconcelos et al, 2014), antikapang (Amano, Katayama, Saito, Ando, & Nagata, 2012;Chikalovets et al, 2015), anti HIV (Alexandre et al, 2011;Zió³kowska & Wlodawer, 2006), antitumor (Hamid, Masood, Wani, & Rafiq, 2013;Singh & Walia, 2018) maupun antiinsekta (Hamid et al, 2013;Lam & Ng, 2011).…”