Antibacterial activity in human amniotic fluid: relationship to zinc and phosphate

Scane, T. M. N.; Hawkins, D. F.

doi:10.1111/j.1471-0528.1984.tb05920.x

Cited by 25 publications

(20 citation statements)

References 27 publications

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“…We next analyzed the growth of strain NEM316 in human AF. AF has been reported to have antimicrobial properties toward various species of bacteria due to β-lysin and lysozyme activity, which depends on divalent cations such as zinc and phosphate [14], [15]. In multiple studies reported in the literature 18–73% of AF samples exhibited inhibitory properties towards various bacterial species.…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid

et al. 2009

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Background Streptococcus agalactiae (group B Streptococcus) is a bacterial pathogen that causes severe intrauterine infections leading to fetal morbidity and mortality. The pathogenesis of GBS infection in this environment is poorly understood, in part because we lack a detailed understanding of the adaptation of this pathogen to growth in amniotic fluid. To address this knowledge deficit, we characterized the transcriptome of GBS grown in human amniotic fluid (AF) and compared it with the transcriptome in rich laboratory medium.MethodsGBS was grown in Todd Hewitt-yeast extract medium and human AF. Bacteria were collected at mid-logarithmic, late-logarithmic and stationary growth phase. We performed global expression microarray analysis using a custom-made Affymetrix GeneChip. The normalized hybridization values derived from three biological replicates at each growth point were obtained. AF/THY transcript ratios representing greater than a 2-fold change and P-value exceeding 0.05 were considered to be statistically significant.Principal FindingsWe have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. GBS grew rapidly in human AF and did not exhibit a global stress response. The majority of changes in GBS transcripts in AF compared to THY medium were related to genes mediating metabolism of amino acids, carbohydrates, and nucleotides. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. Importantly, the response to growth in human AF included significant changes in transcripts of multiple virulence genes such as adhesins, capsule, and hemolysin and IL-8 proteinase what might have consequences for the outcome of host-pathogen interactions.Conclusions/SignificanceOur work provides extensive new information about how the transcriptome of GBS responds to growth in AF, and thus new leads for pathogenesis research.

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Section: Resultsmentioning

confidence: 99%

Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid

et al. 2009

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“…Studies have reported that amniotic fluid inhibits the growth of E. coli and other bacteria because of the presence of lysozyme, transferrin, immunoglobulins (IgA and IgG, but not IgM), zinc and phosphate, and lipid-rich substances [319][320][321][322][323][324][325]. The addition of meconium to amniotic fluid in vitro has resulted in increased growth of E. coli and GBS in some studies [326,327].…”

Section: Pathogenesismentioning

confidence: 99%

Bacterial Sepsis and Meningitis

Nizet¹,

Klein²

2011

Infectious Diseases of the Fetus and Newborn

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“…Phosphate-to-zinc ra tio has been reported to correlate directly to bacterial growth inhibition in A F [6]. In some other investigations [7][8][9] this correla tion was not found. We have developed a rapid micromethod for assessing antibacte rial activity in A F [10].…”

Section: Introductionmentioning

confidence: 80%

Trace Elements and Antibacterial Activity in Amniotic Fluid

Honkonen¹,

Nántö

Hyörä

et al. 1986

Neonatology

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Antibacterial activity and trace element concentrations in amniotic fluid (AF) were determined in a population of 39 pregnant women in the second half of gestation. Antibacterial activity in each AF was measured by a spectrophotometric micromethod after 18 h incubation at 37 °C using Escherichia coli K 12 as a reference bacterium. Concentrations of zinc, iron, copper, calcium, potassium and bromine were measured by particle-induced X-ray emission method and the zinc concentration was also measured by atomic absorption spectrophotometry. Phosphate concentration was determined by direct albumin adding method. In AFs with good antibacterial activity significantly lower concentrations of potassium and bromine were found when compared to AFs with lower antibacterial activity. Concentrations of zinc, iron, copper, calcium or phosphate did not correlate with antibacterial activity in AF.

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Antibacterial activity in human amniotic fluid: relationship to zinc and phosphate

Cited by 25 publications

References 27 publications

Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid

Transcriptome Adaptation of Group B Streptococcus to Growth in Human Amniotic Fluid

Bacterial Sepsis and Meningitis

Trace Elements and Antibacterial Activity in Amniotic Fluid

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