Antibacterial Activity, Cytotoxicity and Mechanisms of action of Cathelicidin Peptides against Enteric Pathogens in Weaning Piglets

Liu, Yifan; Luan, Chao; Xia, Xi; An, Shuhong; Wang, Yizhen

doi:10.1007/s10989-011-9255-y

Cited by 19 publications

(17 citation statements)

References 27 publications

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“…Cathelicidin-BF displayed effective antimicrobial activities against ciprofloxacin-resistant Escherichia coli and Staphylococcus aureus [19]. In addition, C-BF displayed higher antibacterial activity against enteric pathogens isolated from the feces of diarrheal weaned piglets than other cathelicidin peptides [12]. However, few studies have investigated the effects of C-BF on intestinal inflammation, let alone inflammation caused by weaning stress.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

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Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κB signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets

Zhang

et al. 2015

International Immunopharmacology

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Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…Cathelicidins are considered to be important and effective components of the innate host defense against microorganisms, and bacterial resistance is not easily developed [11][12][13]. Cathelicidins are able to directly kill or inactivate some antibiotic-resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κB signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets

Zhang

et al. 2015

International Immunopharmacology

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“…Cathelicidin-BF (C-BF) was the first cathelicidin family peptide found in reptiles and has been found to exerting potent antibacterial activity against gram-negative bacteria, especially to Salmonella. Minimal inhibitory concentration (MIC) of C-BF to S. typhimurium is 4 g/mL, far more effective than the human cathelicidin peptide LL-37 (MIC = 128 g/mL) (Liu et al 2011). C-BF is clearly among the most potent cathelicidins discovered to date.…”

Section: Introductionmentioning

confidence: 99%

The antimicrobial peptide cathelicidin-BF could be a potential therapeutic for Salmonella typhimurium infection

Xia

Lin

Wang

2015

Microbiological Research

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Resistance is increasing to several critical antimicrobials used to treat Salmonella typhimurium infection, urging people to search for new antimicrobial agents. In this work, we reported the possibility of a potent antimicrobial peptide cathelicidin-BF found in the venom of the snake Bungarus fasciatus in treating Salmonella typhimurium infection. We tested its activity in biological fluids and in vivo using a mouse model of Salmonella typhimurium infection, and examined the effect of cathelicidin-BF on Salmonella invasion to epithelial cells. In addition, the biodistribution of cathelicidin-BF was evaluated by using in vivo optical imaging. The results revealed that cathelicidin-BF was unstable in gastrointestinal tract, but retained substantially active in murine serum. Cathelicidin-BF attenuated the clinical symptoms of Salmonella infected-mice, significantly reduced the number of internalized Salmonella and attenuated Salmonella-induced decreases in TER in epithelial cells. Our results provide a first indication for the potential of cathelicidin-BF as a novel therapeutic option for salmonellosis.

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“…Recent studies have shown that cathelicidin peptides have protective effects on intestinal epithelial barrier function by increasing the expression of TJ proteins (16,17). Cathelicidin-WA (CWA), a novel cathelicidin peptide from snakes, has been shown to exhibit strong antimicrobial activities against Gram-negative enteric bacteria (18,19). Additionally, CWA can attenuate intestinal inflammation via immune regulation, independently of its antibacterial activity (10,14).…”

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Cathelicidin-WA Improves Intestinal Epithelial Barrier Function and Enhances Host Defense against Enterohemorrhagic Escherichia coli O157:H7 Infection

Chen

et al. 2017

The Journal of Immunology

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Impaired epithelial barrier function disrupts immune homeostasis and increases inflammation in intestines, leading to many intestinal diseases. Cathelicidin peptides suppress intestinal inflammation and improve intestinal epithelial barrier function independently of their antimicrobial activity. In this study, we investigated the effects of Cathelicidin-WA (CWA) on intestinal epithelial barrier function, as well as the underlying mechanism, by using enterohemorrhagic Escherichia coli (EHEC)-infected mice and intestinal epithelial cells. The results showed that CWA attenuated EHEC-induced clinical symptoms and intestinal colitis, as did enrofloxacin (Enro). CWA decreased IL-6 production in the serum, jejunum, and colon of EHEC-infected mice. Additionally, CWA alleviated the EHEC-induced disruption of mucin-2 and goblet cells in the intestine. Interestingly, CWA increased the mucus layer thickness, which was associated with increasing expression of trefoil factor 3, in the jejunum of EHEC-infected mice. CWA increased the expression of tight junction proteins in the jejunum of EHEC-infected mice. Using intestinal epithelial cells and a Rac1 inhibitor in vitro, we demonstrated that the CWA-mediated increases in the tight junction proteins might depend on the Rac1 pathway. Furthermore, CWA improved the microbiota and short-chain fatty acid concentrations in the cecum of EHEC-infected mice. Although Enro and CWA had similar effects on intestinal inflammation, CWA was superior to Enro with regard to improving intestinal epithelial barrier and microbiota in the intestine. In conclusion, CWA attenuated EHEC-induced inflammation, intestinal epithelial barrier damage, and microbiota disruption in the intestine of mice, suggesting that CWA may be an effective therapy for many intestinal diseases.

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Antibacterial Activity, Cytotoxicity and Mechanisms of action of Cathelicidin Peptides against Enteric Pathogens in Weaning Piglets

Cited by 19 publications

References 27 publications

Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κB signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets

Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κB signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets

The antimicrobial peptide cathelicidin-BF could be a potential therapeutic for Salmonella typhimurium infection

Cathelicidin-WA Improves Intestinal Epithelial Barrier Function and Enhances Host Defense against Enterohemorrhagic Escherichia coli O157:H7 Infection

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