Abstract:The study was aimed at the in-vitro investigation of the antibacterial activity, antioxidant potential and bioactive compound isolation from ethyl acetate crude fraction of Laggera aurita (L. aurita) Linn. The crude fraction was tested against five gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Proteus mirabilis) and three gram positive bacteria (Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis) using macro dilution technique. The an… Show more
Senna alata is a plant used for medical purposes, and its leaves have an extended history of use as a traditional herbal medicine in Indonesia. S. alata is known to contain some components of biologically active compounds and also secondary metabolites. In fact, S. alata can grow well in various locations in Indonesia, location differences can lead to differences in compound content due to differences in environmental conditions such as soil, rainfall, light intensity, and humidity. Therefore, this study aimed to analyze marker compounds of S. alata plants origin from three different location in East Kalimantan, i.e Samarinda, Samboja, and Berau. This research was conducted to estimate the compounds contained in extracts by using GC-MS analysis, and to discover relationships between different variables by using Principal Component Analysis (PCA). There are variances in the yield of secondary metabolites according on where Senna alata is grown, specifically in the riverside Nyapa Indah region and the plains of Samarinda and Samboja. Based on GC-MS test results, Phytol was the main compound in S.alata in two areas, i.e. Samarinda and Samboja. Meanwhile, in Berau showed that 1,2-Benzenedicarboxylic acid, and mono (2-ethylhexyl) ester were the main components. However, S. alata leaf extracts could be used as a good quality raw material for pharmaceutical industries, such as a laxative agent.
Senna alata is a plant used for medical purposes, and its leaves have an extended history of use as a traditional herbal medicine in Indonesia. S. alata is known to contain some components of biologically active compounds and also secondary metabolites. In fact, S. alata can grow well in various locations in Indonesia, location differences can lead to differences in compound content due to differences in environmental conditions such as soil, rainfall, light intensity, and humidity. Therefore, this study aimed to analyze marker compounds of S. alata plants origin from three different location in East Kalimantan, i.e Samarinda, Samboja, and Berau. This research was conducted to estimate the compounds contained in extracts by using GC-MS analysis, and to discover relationships between different variables by using Principal Component Analysis (PCA). There are variances in the yield of secondary metabolites according on where Senna alata is grown, specifically in the riverside Nyapa Indah region and the plains of Samarinda and Samboja. Based on GC-MS test results, Phytol was the main compound in S.alata in two areas, i.e. Samarinda and Samboja. Meanwhile, in Berau showed that 1,2-Benzenedicarboxylic acid, and mono (2-ethylhexyl) ester were the main components. However, S. alata leaf extracts could be used as a good quality raw material for pharmaceutical industries, such as a laxative agent.
Objectives:
Essential oils and extracts from medicinal plants have been shown to have antimicrobial properties in several investigations carried out in regions with diverse floras. This study intends to evaluate the antimicrobial activity of Azadirachta indica (Neem plant) bark extract on microbial isolates.
Materials and Methods:
The plant’s bark was cut out of the tree, dried, and pulverized using a mechanical grinder. The crushed barks were split in half, one half macerated in ethanol and the other put through the Soxhlet apparatus. The ethanol extract of plant bark was used to analyze microbial isolates (Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Candida albicans). The active components in the extracts were analyzed using gas chromatography-mass spectrometry.
Results:
According to the inhibition zone width, mean inhibition concentration, and lowest bactericidal concentration, all organisms were shown to be sensitive to the antibacterial activities of A. indica at varied doses of the extracts utilized. For every isolate examined, the minimum inhibitory concentration (MIC) of the extract was 12.5 mg/mL; however, B. subtilis had a concentration of 25 mg/mL. The extract had bactericidal activity on all the isolates except Bacillus sp. The minimum bactericidal concentration (MBC) for the isolates was 12.5 mg/mL for P. aeruginosa, S. aureus, and C. albicans, and 100 mg/mL for E. coli. Among the principal compounds discovered are pentadecanoic acid, 14-methyl-methyl ester, stigmasterol, 9-octadecanoic acid (z)-methyl ester, methyl stearate, n-hexadecanoic acid, linoelaidic acid, and Vitamin E.
Conclusion:
Our research showed that the ethanol extract from A. indica bark contains several bioactive compounds with antimicrobial properties.
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