Antibacterial Activity and Phytochemical Screening of Wild Edible Mushroom Pleurotus Ostreatuscollected From Himachal Pradesh

Abstract

The objective of present study was to identify the unknown wild edible mushroom based on morphological characteristics and DNA based sequencing by using universal primers ITS 1and ITS 4, antibacterial activity, phytochemical screening and FTIR analysis of mushroom extract spectra.Methods:-The wild edible mushroom specimen was tentatively identified morphologically and was cultured on 2% MEA. The isolate thus obtained was screened for its antimicrobial activity. Methanol extract was prepared in order to carry out antibacterial activity. The extract was subjected to qualitative phytochemicaland FTIR analysis.Results:-The isolates on the basis of morphological and molecular characterization was identified as P. ostreatus. Agar-well diffusion method was used to carry out antimicrobial assay. The methanolextract ofP. ostreatus showed antimicrobial activity against E.coli having zone inhibition diameter 24.3±0.5, against S. aureus having zone inhibition diameter 26.6±0.5,againstP. aeruginosa having zone inhibition diameter20.3±0.5 and against K.pneumoniae having zone inhibition diameter 28.6±0.5. Fourier transform infrared (FT-IR) analysis indicated that all mushroom's extract spectra showed typical carbohydrate pattern with strong and broad absorption near 3300 cm -1 which indicates the presence of OH group. Conclusion:-The isolate analysis have been showed to be good source of antimicrobial agent and phytoconstituents, therefore could be potential source of bioactive compounds with beneficial biological activities. .

Validating Anti-Infective Activity of Pleurotus Opuntiae via Standardization of Its Bioactive Mycoconstituents through Multimodal Biochemical Approach

“…The findings obtained from present study affirm this further with a focus on antibacterial activity. Similar antimicrobial potentials have been observed in P. ostreatus [27] and many other species [21] but have never been reported in P. opuntiae despite having medicinal properties. Bacterial strains included in this study were chosen based on their significance and etiology in clinical microbiology and in different infectious processes [28].…”

“…The presence of a higher quantity of compounds in the ethanol extract of P. opuntiae is responsible for the antimicrobial activity at lower MIC values against test microorganisms [31,32]. The preliminary screening tests can be helpful in the identification of bioactive compounds in the mushroom and can help in the discovery and production of drugs [21]. Groups of mycochemical compounds are commonly associated with combating microbial resistance and having antimicrobial behavior in edible mushroom.…”

“…The methanol and ethanol extracts of P. opuntiae were screened to detect the presence of mycoconstituents such as alkaloids, carbohydrate, phenols, diterpenes, phytosterols (triterpenes), proteins, amino acids, tannins, anthraquinones, flavonoids, phlobatamins, terpenes, cardiac glycosides, saponins following standard protocols [20,21].…”

Mushroom consumption pattern among residents of Ibadan Metropolis in Oyo State, Nigeria

“…Cholesterol and the sterol known to be dreaded for the heart are absent in mushrooms (Chaube, cited in Yehuala, 2012). Fresh mushrooms contain a relatively large amount of carbohydrate (4-5%), fiber and significant amounts of phosphorous, sodium and potassium but lesser amounts of calcium and iron (Bains and Tripathi, 2016). They have been prized as a delicacy for more than 2000 years now (Quimio et al, 1990;Buah et al, 2010).…”