Antibacterial activity and mechanism of chloroform fraction from aqueous extract of mugwort leaves (Artemisia argyi L.) against Staphylococcus aureus

Qu, Lingbo; Bi, Ye-Feng; Pan, Cheng-Xue; Yang, Ran; Zeng, Hua‐jin

doi:10.1111/lam.13684

Cited by 14 publications

(11 citation statements)

References 16 publications

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“…Although the bioactive ingredients of A. argyi exhibit various pharmacological and therapeutic functions, few studies have investigated the anti-infectious diseases of A. argyi [ 11 , 12 ]. We presented here that AEE inhibits HSV-1 and HSV-2 infection and exerts inhibitory function against ACV-resistant HSV-1 strains, implying a novel antiviral mechanism different from ACV.…”

Section: Discussionmentioning

confidence: 99%

“…A. argyi has been historically used to warm menstruation, relieve pain, disperse dampness and cold, and regulate Qi and blood. Up to now, several volatile oils, terpenoids, flavonoids, alkaloids, and polysaccharides have been isolated from A. argyi extract, which demonstrates antioxidative [ 7 ], anti-cancer [ 8 , 9 ], anti-inflammatory [ 10 ], antibacterial [ 11 , 12 ] and immunosuppressive [ 13 ] effects. Notably, most studies focus on the aqueous extract and essential oil component of A. argyi .…”

Section: Introductionmentioning

confidence: 99%

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Ethanol extract from Artemisia argyi leaves inhibits HSV-1 infection by destroying the viral envelope

Liu

Zhong

Xiao

et al. 2023

Virol J

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Herpes simplex virus type 1 (HSV-1) is a widely disseminated virus that establishes latency in the brain and causes occasional but fatal herpes simplex encephalitis. Currently, acyclovir (ACV) is the main clinical drug used in the treatment of HSV-1 infection, and the failure of therapy in immunocompromised patients caused by ACV-resistant HSV-1 strains necessitates the requirement to develop novel anti-HSV-1 drugs. Artemisia argyi, a Traditional Chinese Medicine, has been historically used to treat inflammation, bacterial infection, and cancer. In this study, we demonstrated the antiviral effect and mechanism of ethanol extract of A. argyi leaves (hereafter referred to as ‘AEE’). We showed that AEE at 10 μg/ml exhibits potent antiviral effects on both normal and ACV-resistant HSV-1 strains. AEE also inhibited the infection of HSV-2, rotavirus, and influenza virus. Transmission electron microscopy revealed that AEE destroys the membrane integrity of HSV-1 viral particles, resulting in impaired viral attachment and penetration. Furthermore, mass spectrometry assay identified 12 major components of AEE, among which two new flavones, deoxysappanone B 7,3ʹ-dimethyl ether, and 3,7-dihydroxy-3′,4ʹ-dimethoxyflavone, exhibited the highest binding affinity to HSV-1 glycoprotein gB at the surface site critical for gB–gH–gL interaction and gB-mediated membrane fusion, suggesting their involvement in inactivating virions. Therefore, A. argyi is an important source of antiviral drugs, and the AEE may be a potential novel antiviral agent against HSV-1 infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ethanol extract from Artemisia argyi leaves inhibits HSV-1 infection by destroying the viral envelope

Liu

Zhong

Xiao

et al. 2023

Virol J

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“…In order to slow down or repress the evolution of bacterial resistance, the development of new antibacterial Chinese herbal medicines and their preparations have become an important hotspot in the field of antibacterial drug research and development. For example, Zhang et al [ 27 ] found that the aqueous extract of Artemisia argyi leaves could destroy the integrity of the cell wall of S. aureus and increase the permeability of the cell membrane at a certain concentration, but it could not kill S. aureus in a short time; Zhou et al [ 28 ] found that a part of n-butanol and ethanol crude extract of the Dendrobium shell had strong antibacterial activity against Salmonella paratyphimurium and S. aureus , and the antibacterial mechanism might be related to the synthesis inhabitation of bacterial proteins. However, both Chinese herbal compound preparations and single Chinese herbal medicines all have complex chemical components, leading to unclear antibacterial mechanisms and lack of standard drug use specifications.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity and Mechanism of Madecassic Acid against Staphylococcus aureus

2023

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Antibacterial resistance has become one of the most serious problems threating global health. To overcome this urgent problem, many scientists have paid great attention to developing new antibacterial drugs from natural products. Hence, for exploring new antibacterial drugs from Chinese medicine, a series of experiments were carried out for verifying and elucidating the antibacterial activity and mechanisms of madecassic acid (MA), which is an active triterpenoid compound isolated from the traditional Chinese medicine, Centella asiatica. The antibacterial activity was investigated through measuring the diameter of the inhibition zone, the minimum inhibitory concentration (MIC), the growth curve, and the effect on the bacterial biofilm, respectively. Meanwhile, the antibacterial mechanism was also discussed from the aspects of cell wall integrity variation, cell membrane permeability, and the activities of related enzymes in the respiratory metabolic pathway before and after the intervention by MA. The results showed that MA had an inhibitory effect on eight kinds of pathogenic bacteria, and the MIC values for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Bacillus megaterium were 31.25, 62.5, 250, 125, 62.5, and 62.5 µg/mL, respectively. For instance, 31.25 µg/mL MA could inhibit the growth of Staphylococcus aureus within 28 h. The antibacterial mechanism experiments confirmed that MA could destroy the integrity of the cell membrane and cell wall of Staphylococcus aureus, causing the leakage of macromolecular substances, inhibiting the synthesis of soluble proteins, reducing the activities of succinate dehydrogenase and malate dehydrogenase, and interacting with DNA, leading to the relaxation and ring opening of supercoiled DNA. Besides, the activities of DNA topoisomerase I and II were both inhibited by MA, which led to the cell growth of Staphylococcus aureus being repressed. This study provides a theoretical basis and reference for the application of MA in the control and inhibition of food-borne Staphylococcus aureus.

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“…Outbreaks of food poisoning events due to microbes tend to increase in recent years. Among them, Staphylococcus aureus ( S. aureus ) accounts for 25% of the total, and it is the third largest microbial pathogen after Haemophilus parahaemolyticus and Salmonella [ 1 ]. As a common foodborne pathogen, S. aureus can grow in a wide range of temperatures and pH.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Efficacy and Mechanisms of Curcumin-Based Photodynamic Treatment against Staphylococcus aureus and Its Application in Juices

et al. 2022

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Antimicrobial Photodynamic Treatment (aPDT) is a non-thermal sterilization technology, which can inactivate common foodborne pathogens. In the present study, photodynamic inactivation on Staphylococcus aureus (S. aureus) with different concentrations of curcumin and light dose was evaluated and the mechanisms were also investigated. The results showed that curcumin-based aPDT could inactivate S. aureus cells by 6.9 log CFU/mL in phosphate buffered saline (PBS). Moreover, the modified Gompertz model presented a good fit at the inactivation data of S. aureus. Photodynamic treatment caused cell membrane damage as revealed by analyzing scanning electron microscopy (SEM) images. Leakage of intracellular constituents further indicated that cell membrane permeability was changed. Flow cytometry with double staining demonstrated that cell membrane integrity and the activity of nonspecific esterase were destroyed. Compared with the control group, intracellular reactive oxygen species (ROS) levels caused by photodynamic treatment significantly increased. Furthermore, curcumin-based aPDT reduced S. aureus by 5 log CFU/mL in juices. The color of the juices was also tested using a Chromatic meter, and it was found that b* values were the most markedly influenced by photodynamic treatment. Overall, curcumin-based aPDT had strong antibacterial activity against S. aureus. This approach has the potential to remove foodborne pathogens from liquid food.

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Antibacterial activity and mechanism of chloroform fraction from aqueous extract of mugwort leaves (Artemisia argyi L.) against Staphylococcus aureus

Cited by 14 publications

References 16 publications

Ethanol extract from Artemisia argyi leaves inhibits HSV-1 infection by destroying the viral envelope

Ethanol extract from Artemisia argyi leaves inhibits HSV-1 infection by destroying the viral envelope

Antibacterial Activity and Mechanism of Madecassic Acid against Staphylococcus aureus

Antibacterial Efficacy and Mechanisms of Curcumin-Based Photodynamic Treatment against Staphylococcus aureus and Its Application in Juices

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