Antibacterial activity and immune responses of a molluscan macrophage expressed gene-1 from disk abalone, Haliotis discus discus

Bathige, S.D.N.K.; Umasuthan, Navaneethaiyer; Whang, Ilson; Lim, Bong-Soo; Won, Seung Hwan

doi:10.1016/j.fsi.2014.05.012

Cited by 29 publications

(31 citation statements)

References 32 publications

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“…For example, Perforin-2 has been shown to limit the burden of Mycobacterium marinum and S. typhimurium in zebrafish embryos ( Benard et al, 2014 ). Additional studies indicate that Perforin-2 orthologs provide similar bactericidal activity in invertebrates ( He et al, 2011 ; Kemp and Coyne, 2011 ; Bathige et al, 2014 ).…”

Section: Discussionmentioning

confidence: 97%

“…Although ubiquitylation was not investigated, upregulation of MPEG1.2 has been shown to require NFκB and the TLR adaptor MyD88 ( Benard et al, 2014 ). In non-vertebrate species the expression of Perforin-2 orthologs is constitutive in some tissues and/or inducible by pathogenic bacteria or LPS ( He et al, 2011 ; Kemp and Coyne, 2011 ; Bathige et al, 2014 ). In addition to similar patterns of expression, the function of Perforin-2 is also conserved across species.…”

Section: Discussionmentioning

confidence: 99%

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Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2

McCormack

Lyapichev

Olsson

et al. 2015

eLife

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Perforin-2 (MPEG1) is an effector of the innate immune system that limits the proliferation and spread of medically relevant Gram-negative, -positive, and acid fast bacteria. We show here that a cullin-RING E3 ubiquitin ligase (CRL) complex containing cullin-1 and βTrCP monoubiquitylates Perforin-2 in response to pathogen associated molecular patterns such as LPS. Ubiquitylation triggers a rapid redistribution of Perforin-2 and is essential for its bactericidal activity. Enteric pathogens such as Yersinia pseudotuberculosis and enteropathogenic Escherichia coli disarm host cells by injecting cell cycle inhibiting factors (Cifs) into mammalian cells to deamidate the ubiquitin-like protein NEDD8. Because CRL activity is dependent upon NEDD8, Cif blocks ubiquitin dependent trafficking of Perforin-2 and thus, its bactericidal activity. Collectively, these studies further underscore the biological significance of Perforin-2 and elucidate critical molecular events that culminate in Perforin-2-dependent killing of both intracellular and extracellular, cell-adherent bacteria.DOI: http://dx.doi.org/10.7554/eLife.06505.001

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2

McCormack

Lyapichev

Olsson

et al. 2015

eLife

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“…Nevertheless, the role of LBP/BPI in anti-trematode defense remains unclear. A second antimicrobial protein was identified as putative Macrophage expressed gene-1 (MPEG1) belonging to the MACPF (membrane-attack complex/perforin) protein superfamily [121]. The corresponding gene was upregulated in hemocytes from resistant snails challenged with S. mansoni [53].…”

Section: -Ros and Rnsmentioning

confidence: 99%

Advances in gastropod immunity from the study of the interaction between the snail Biomphalaria glabrata and its parasites: A review of research progress over the last decade

Coustau

Gourbal

Duval

et al. 2015

Fish & Shellfish Immunology

104

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This review summarizes the research progress made over the past decade in the field of gastropod immunity resulting from investigations of the interaction between the snail Biomphalaria glabrata and its trematode parasites. A combination of integrated approaches, including cellular, genetic and comparative molecular and proteomic approaches have revealed novel molecular components involved in mediating Biomphalaria immune responses that provide insights into the nature of host-parasite compatibility and the mechanisms involved in parasite recognition and killing. The current overview emphasizes that the interaction between B. glabrata and its trematode parasites involves a complex molecular crosstalk between numerous antigens, immune receptors, effectors and anti-effector systems that are highly diverse structurally and extremely variable in expression between and within host and parasite populations. Ultimately, integration of these molecular signals will determine the outcome of a specific interaction between a B. glabrata individual and its interacting trematodes. Understanding these complex molecular interactions and identifying key factors that may be targeted to impairment of schistosome development in the snail host is crucial to generating new alternative schistosomiasis control strategies.

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“…Interestingly, one transcript putatively coding for a macrophage-expressed gene 1 (MPEG1) protein and multiple laccase transcripts were found to be 2-fold and higher down-regulated in the pCO 2 stressed S. glomerata (Additional file 7: Table S2). MPEG1 of the disk abalone Haliotis discus discus showed antibacterial activity against Gram-positive and -negative bacteria and was up-regulated in response to bacteria and viral hemorrhagic septicaemia virus [52]. Similarly, a laccase of the sponge Suberites domuncula was up-regulated in response to bacterial lipopolysaccharide and also showed antibacterial activity when the laccase mediator ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] was present [53].…”

Section: Immunitymentioning

confidence: 99%

Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response

Ertl

O’Connor

Wiegand

et al. 2016

Clim Chang Responses

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Background: Human activities have led to a substantial increase in carbon dioxide (CO 2 ) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated pCO 2 . Results: A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated pCO 2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO 2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO 2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions: This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO 2 .

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Antibacterial activity and immune responses of a molluscan macrophage expressed gene-1 from disk abalone, Haliotis discus discus

Cited by 29 publications

References 32 publications

Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2

Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2

Advances in gastropod immunity from the study of the interaction between the snail Biomphalaria glabrata and its parasites: A review of research progress over the last decade

Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response

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