Antibacterial activity and antibiotic-modifying action of carvacrol against multidrug-resistant bacteria

Silva, Ana Raquel Pereira da; Costa, Maria do Socorro; Araújo, Nara Juliana Santos; Freitas, Thiago Sampaio de; Santos, Antônia Thassya Lucas dos; Gonçalves, Sara; Silva, Viviane Bezerra da; Andrade-Pinheiro, Jacqueline Cosmo; Tahim, Catarina Martins; Lucetti, Elaine Cristina Pereira; Coutinho, Henrique Douglas Melo

doi:10.1016/j.sampre.2023.100072

Cited by 3 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the compounds in M. chinensis, the bioactive thymol and carvacrol had significant antimicrobial activity which inhibited microbial growth by destroying the cellular structure (cell membrane) of microorganisms, resulting in the leakage of essential molecules such as protein and K + [18]. Carvacrol has demonstrated relevant clinical antibacterial activity, for both Gram-positive and Gram-negative bacteria, and a synergistic effect when associated with gentamicin [19]. Therefore, the antibacterial results provide valuable information for the development of relevant drugs and cosmetics originating from M. chinensis in the future.…”

Section: Antibacterial Activity Of Extract Sample From Sfe-comentioning

confidence: 99%

HPLC–DAD Analysis, SFE-CO2 Extraction, and Antibacterial Activity on Bioactive Compounds from Mosla chinensis Maxim

Gao,

Han,

Zeng

et al. 2023

Molecules

View full text Add to dashboard Cite

Mosla chinensis Maxim is an annual herb with many potential purposes in agricultural, industrial, and pharmaceutical fields. At present, the extract of the whole plant from M. chinensis has been proven to demonstrate antifungal, antioxidant, and anti-inflammatory activities. Previous studies focused on the enzyme pretreatment in hydrodistillation from M. chinensis. However, organic solvent or supercritical fluid carbon dioxide extraction (SFE-CO2) methods, which are commonly utilized in industry, have seldom been studied and cannot provide multiple evaluations of yield. In this work, we analysed compounds from M. chinensis by HPLC–DAD, discussed n-hexane extraction, and conducted further investigations on SFE-CO2 through the design of response surface methodology (RSM). The sample obtained from pilot-scale SFE-CO2 was also tested against nine kinds of microorganisms. Single-factor results revealed that the extraction rates from M. chinensis by steam distillation, n-hexane extraction, and SFE-CO2 were 1%, 2.09%, and 3.26%, respectively. RSM results showed a significant improvement in extraction rate through optimising pressure and time, and the interaction of both factors was more important than that of temperature–pressure and temperature–time. A pilot-scale test with an extraction rate of 3.34% indicated that the predicted RSM condition was operable. In addition, samples from the pilot-scale SFE-CO2 showed antibacterial effects against three previously unreported bacteria (Gardnerella vaginalis, methicillin-resistant Staphylococcus aureus, and Propionibacterium acnes). These results fill the gap in previous research and provide more information for the application and development of M. chinensis in the future.

show abstract

Section: Antibacterial Activity Of Extract Sample From Sfe-comentioning

confidence: 99%

HPLC–DAD Analysis, SFE-CO2 Extraction, and Antibacterial Activity on Bioactive Compounds from Mosla chinensis Maxim

Gao,

Han,

Zeng

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…Similarly, bacterial infections are also a major burden to the public health system globally. The issue of antibiotic resistance is a risk factor that compromises the efficacy of most antibacterial drugs [10][11][12]. More than 700,000 patients die yearly due to a lack of effective treatments, with an estimated 10 million lives being lost due to antibiotic-resistant pathogens each year.…”

Section: Introductionmentioning

confidence: 99%

Carvacrol and Thymol Hybrids: Potential Anticancer and Antibacterial Therapeutics

Peter,

Sotondoshe,

Aderibigbe

2024

Molecules

View full text Add to dashboard Cite

Cancer is ranked among lethal diseases globally, and the increasing number of cancer cases and deaths results from limited access to effective therapeutics. The use of plant-based medicine has been gaining interest from several researchers. Carvacrol and its isomeric compound, thymol, are plant-based extracts that possess several biological activities, such as antimalarial, anticancer, antifungal, and antibacterial. However, their efficacy is compromised by their poor bioavailability. Thus, medicinal scientists have explored the synthesis of hybrid compounds containing their pharmacophores to enhance their therapeutic efficacy and improve their bioavailability. Hence, this review is a comprehensive report on hybrid compounds containing carvacrol and its isomer, thymol, with potent anticancer and antibacterial agents reported between 2020 and 2024. Furthermore, their structural activity relationship (SAR) and recommended future strategies to further enhance their therapeutic effects will be discussed.

show abstract

“…Furthermore, an important aspect to highlight is that the development and application of highly effective natural anti-biofilm treatments for biofilm-related issues in food products and packaging are crucial, given the increasing microbial resistance to antibiotics (Burt, 2004;Asma et al, 2022). This is particularly noteworthy as it has been demonstrated that carvacrol can inhibit microbial growth and biofilm formation without promoting antimicrobial resistance (da Silva et al, 2023). Innovative approaches to assess the culturability and viability of food pathogens in complex matrices are needed to optimize the protocol used for food production and evaluate the effectiveness of preservation treatments (Fleischmann et al, 2021;Rubbens and Props, 2021).…”

Section: Introductionmentioning

confidence: 99%

Lactiplantibacillus plantarum monolayer enhanced bactericidal action of carvacrol: biofilm inhibition of viable foodborne pathogens and spoilage microorganisms

Poscente,

Di Gregorio,

Costanzo

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The prevalence of biofilm-associated microorganisms and the increasing use of ready-to-eat fresh products represent the current duality the food industry must address. Innovative and eco-friendly antibiofilm solutions and appropriate microbiological food control systems are urgently needed to improve food quality and safety. This study aimed to investigate the in vitro combined efficacy of carvacrol with a pre-formed biofilm monolayer of the probiotic Lactiplantibacillus plantarum DSM 20174. The antimicrobial activity of carvacrol against both planktonic and sessile cells of foodborne pathogens and spoilage microorganisms, alone or in the presence of the pre-formed biofilm of L. plantarum, was investigated by culture-based methods along with flow cytometry (FCM) to monitor cells' cultivability and viability. The synergistic action of carvacrol and the pre-formed biofilm of L. plantarum was evaluated in the 96-well plates. The results showed that L. plantarum pre-formed biofilm monolayer enhanced the antimicrobial effect of carvacrol determining a bactericidal action while the treatment alone induced the viable but not culturable (VBNC) cell state only. Furthermore, the great efficacy of the combined treatment allowed the application of a lower concentration of carvacrol (100 ppm) to achieve significant damage in cell viability. In conclusion, the incorporation of carvacrol into the L. plantarum pre-formed biofilm represents a promising alternative for an antimicrobial functionalized ready-to-eat packaging.

show abstract

Antibacterial activity and antibiotic-modifying action of carvacrol against multidrug-resistant bacteria

Cited by 3 publications

References 24 publications

HPLC–DAD Analysis, SFE-CO2 Extraction, and Antibacterial Activity on Bioactive Compounds from Mosla chinensis Maxim

HPLC–DAD Analysis, SFE-CO2 Extraction, and Antibacterial Activity on Bioactive Compounds from Mosla chinensis Maxim

Carvacrol and Thymol Hybrids: Potential Anticancer and Antibacterial Therapeutics

Lactiplantibacillus plantarum monolayer enhanced bactericidal action of carvacrol: biofilm inhibition of viable foodborne pathogens and spoilage microorganisms

Contact Info

Product

Resources

About