Abstract:The study aimed to determine the phytochemicals and to assess the antibacterial activities of crude extracts of different parts of Calpurnia aurea against Staphylococcus aureus, Streptococcus pyogenes, Listeria monocytogenes, Escherichia coli O157 H:7, Salmonella typhi, and Campylobacter jejuni. The fresh and healthy leaves, barks, stems, and roots of the plant parts were collected, herbarium, dried, and grounded, and bioactive compounds were extracted by ethanol (99%) and water. Mass of crude extracts was det… Show more
“…The disks which had been injected with the mushroom extracts were placed on the solid agar medium by pressing gently. Ciprofloxacin 0.2 mg/ml [ 25 ] was used as the standard drug for the test bacteria strains. The Petri dishes which were treated were kept at 4°C for 1-2 hours and thereafter they were incubated at 35°C for 18–24 hours.…”
The mushroom Termitomyces striatus is an edible mushroom that grows wildly and belongs to the family Lyophyllaceae. Studies in the last few decades have demonstrated that mushrooms and their active components have beneficial effects on a variety of biological systems. Some mushrooms do exhibit antibacterial properties. Qualitative phytochemical profile was done on the mushroom Termitomyces striatus to establish the presence of compounds responsible for important biological activities. This study also investigated the effect of Termitomyces striatus extracts on certain bacterial strains that included Escherichia coli and Pseudomonas aeruginosa representing the Gram-negative bacteria and Bacillus subtilis and Staphylococcus aureus representing Gram-positive bacteria. The fungi were represented by Candida albicans and Saccharomyces cerevisiae. The mushroom was collected in western Kenya, air-dried, and crushed into powder, followed by extraction using water, methanol, and dichloromethane (DCM) solvents. Antibacterial and antifungal activities were evaluated using the disc-diffusion method. Qualitative phytochemical screening of the aqueous extract revealed the presence of alkaloids, flavonoids, steroids, sterols, saponins, phenols, carbohydrates, and proteins. The three extracts exhibited antibacterial against tested bacterial strains. The DCM extract revealed higher effects among the bacterial strains tested. The three extracts showed antifungal effects against C. albicans. However, both methanol and aqueous extracts did not inhibit growth of S. cerevisiae. In conclusion, T. striatus extracts are a promising source of novel antimicrobial and antifungal agents.
“…The disks which had been injected with the mushroom extracts were placed on the solid agar medium by pressing gently. Ciprofloxacin 0.2 mg/ml [ 25 ] was used as the standard drug for the test bacteria strains. The Petri dishes which were treated were kept at 4°C for 1-2 hours and thereafter they were incubated at 35°C for 18–24 hours.…”
The mushroom Termitomyces striatus is an edible mushroom that grows wildly and belongs to the family Lyophyllaceae. Studies in the last few decades have demonstrated that mushrooms and their active components have beneficial effects on a variety of biological systems. Some mushrooms do exhibit antibacterial properties. Qualitative phytochemical profile was done on the mushroom Termitomyces striatus to establish the presence of compounds responsible for important biological activities. This study also investigated the effect of Termitomyces striatus extracts on certain bacterial strains that included Escherichia coli and Pseudomonas aeruginosa representing the Gram-negative bacteria and Bacillus subtilis and Staphylococcus aureus representing Gram-positive bacteria. The fungi were represented by Candida albicans and Saccharomyces cerevisiae. The mushroom was collected in western Kenya, air-dried, and crushed into powder, followed by extraction using water, methanol, and dichloromethane (DCM) solvents. Antibacterial and antifungal activities were evaluated using the disc-diffusion method. Qualitative phytochemical screening of the aqueous extract revealed the presence of alkaloids, flavonoids, steroids, sterols, saponins, phenols, carbohydrates, and proteins. The three extracts exhibited antibacterial against tested bacterial strains. The DCM extract revealed higher effects among the bacterial strains tested. The three extracts showed antifungal effects against C. albicans. However, both methanol and aqueous extracts did not inhibit growth of S. cerevisiae. In conclusion, T. striatus extracts are a promising source of novel antimicrobial and antifungal agents.
“…Then, it was ground by using a conventional wooden-made pestle and mortar, pounded using an electric grinder into a fine powder, and finally kept in a refrigerator (4 °C) until used. 36 , 39 …”
Section: Methodsmentioning
confidence: 99%
“…After the solvent was evaporated, the remaining crude extracts were diluted with 10 mL of sterile distilled water and kept in an airtight bottle in the refrigerator until the experiment was carried out. 36 …”
Section: Methodsmentioning
confidence: 99%
“…After the solvent was evaporated, the remaining crude extracts were diluted with 10 mL of sterile distilled water and kept in an airtight bottle in the refrigerator until the experiment was carried out. 36 Preliminary Phytochemical Test metabolites that might be playing a significant role in the antibacterial activities of medicinal plants. Accordingly, each plant was tested for the presence of alkaloids, saponins, phenols, tannins, flavonoids, and phytosterols.…”
Section: Plant Extractionmentioning
confidence: 99%
“…30 Also, the use of medicinal plants to treat infections is an old practice in large parts of Ethiopia to solve health problems for livestock and humans. [31][32][33][34][35][36] However, most of these experiences are not documented and supported by scientific experiments. Among medicinal plants, leaves of S. subserrata, fruits of R. chalepensis, and roots of E. amplexicaulis were frequently used for the treatments of disease-causing bacterial strains like S. aureus, E. coli, S. pneumoniae, and P. aeruginosa.…”
Background: Although traditional healers in Ethiopia have a long history of using medicinal plants to treat diseases in animals and humans, studies on the antibacterial activities and potential bioactive ingredients of most medicinal plants have been insufficient. Therefore, this study aimed to evaluate the in-vitro antibacterial activities and to screen phytochemical constituents of selected medicinal plants against reference bacterial strains. Methods: The fresh and healthy roots of Echinops amplexicaulis, fruits of Ruta chalepensis, and leaves of Salix subserrata were collected from West Shewa Zone, Ethiopia. Agar well diffusion and agar dilution methods were used to evaluate antibacterial activities and minimum inhibitory concentrations (MIC). All the crude plant extracts were tested against Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Pseudomonas aeruginosa at concentrations of 100, 50, and 25 mg/mL in each triplet (3x). MIC of crude extracts ranging from 1.5625 to 12.50 mg/mL was applied to all bacterial strains. The positive control was ciprofloxacin disk (5 μg) and the negative control was 5% dimethyl sulfoxide. The presence of secondary metabolites of each crude extract was screened. The group means comparisons were done using one-way ANOVA and results were presented as mean ± standard deviation. Results: Although all selected plant extracts had shown antibacterial activities, methanol extracts had a greater zone of inhibition against all reference bacterial strains when compared to petroleum ether extracts. The growth of P. aeruginosa was inhibited at a minimum concentration of both methanol and petroleum extracts (1.5625 mg/mL) when compared to the remaining bacterial strains. Phytochemical screening showed that saponins and alkaloids were found in all crude plant extracts, while phytosterol was meager.
Conclusion:This study revealed that all tested plants had significant secondary metabolites and antibacterial activities against reference bacterial strains.
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