Antibacterial activities of Calpurnia aurea and Ocimum lamiifolium extracts against selected gram positive and gram-negative bacteria

Melese, Addis; Dobo, Beyene; Mikru, Abriham

doi:10.4314/ejst.v12i3.2

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…They also help researchers obtain unbiased data from local healers while ensuring that they maintain strict confidentiality about cultural beliefs and practices. This information is valuable in studying medicinal plants [ 32 , 36 ]. However, conservation efforts to preserve these plants are limited in the area, even though the natural forest is rapidly disappearing due to various factors like deforestation [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Ethnobotanical study of traditional medicinal plants used by the local Gamo people in Boreda Abaya District, Gamo Zone, southern Ethiopia

Zemede,

Mekuria,

Ochieng

et al. 2024

J Ethnobiology Ethnomedicine

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Background Medicinal plants have been used for centuries and are still relied upon by over 80% of the Ethiopian population. The people of Gamo, southern Ethiopia, have a rich cultural and traditional lifestyle with a long history of using plant resources for various uses including traditional herbal medicine. However, their traditional knowledge of traditional medicinal plants in Boreda Abaya District has not been explored Ethnobotanically yet, despite preserving diverse indigenous traditional medicinal plants. Hence, the study aimed to document and analyze traditional medicinal plants and associated traditional knowledge and practices used by local people. Materials and methods Quantitative ethnobotanical data were collected via semi-structured interviews, face-to-face conversations, group discussions, and guided field trips between September 2022 and February 2023. In total, 92 informants participated, of which 25 were key informants. Quantitative data indices (informant consensus factor—ICF—and use report—Ur) were computed by MS Excel spreadsheet software. Scientific names of medicinal plants were checked via World Flora Online. Results In the present study, we recorded 188 traditional medicinal plant species belonging to 163 genera and 73 plant families. Lamiaceae (16 species), Asteraceae (16 species), Fabaceae (11 species), and Euphorbiaceae (8 species) contributed highest number of species and were found to be predominant family in the area. Leaves and seeds were most frequently used plant parts, and pounding (46%) was the main method to prepare remedies. The sudden sickness disease category scored the highest consensus (ICF: 0.35), followed by blood and circulatory-related disease categories (ICF: 0.33). The highest number of plant taxa (61 species) used to treat dermal disease has a 71-use report score, while fewer plant taxa (21 species) were utilized to treat genitourinary system-related disease category, having 25 use reports. Ocimum lamiifolium (Ur:56) and Moringa stenopetala (Ur:51) are widely used species and received highest use report value. Conclusion Gamo people possess extensive traditional knowledge of ethnomedicine. The region's vegetation hosts diverse medicinal species, but deforestation, agriculture, and droughts threaten them. Local conservation practices require scientific support, prioritizing species having higher use reports (Ur), and in-depth investigations of promising species for drug development are essential.

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Section: Resultsmentioning

confidence: 99%

Ethnobotanical study of traditional medicinal plants used by the local Gamo people in Boreda Abaya District, Gamo Zone, southern Ethiopia

Zemede,

Mekuria,

Ochieng

et al. 2024

J Ethnobiology Ethnomedicine

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“…The extract had MIC value of greater than 2.5 mg/ml against E. coli . Meles et al 5 , Elisha et al 6 , Mulatu 8 , Bogale 9 and others have reported the antibacterial activities of C. aurea.…”

Section: Discussionmentioning

confidence: 97%

“…They contain phytochemicals 3 , 4 . These phytochemicals have a significant inhibitory effect on the growth of pathogens 5 .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea

Wasihun¹,

Habteweld

Ayenew

2023

Sci Rep

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Local Ethiopians use Calpurnia aurea to treat skin infections. However, there is no adequate scientific confirmation. The aim of this study was to evaluate the antibacterial activities of the crude and the fractionated extracts of C. aurea leaves against different bacterial strains. The crude extract was made by maceration. The Soxhlet extraction method was used to obtain fractional extracts. The antibacterial activity against gram positive and gram negative American Type Culture Collection (ATCC) strains was performed using the agar diffusion technique. The minimum inhibitory concentration was determined through the microtiter broth dilution method. Preliminary phytochemical screening was done using standard techniques. The largest yield was obtained from ethanol fractional extract. Except for chloroform, which provided a relatively low yield when compared to petroleum ether, increasing the polarity of the extracting solvent improved the yield. The crude extract, solvent fractions, and the positive control showed inhibitory zone diameter, while the negative control did not. When used at a concentration of 75 mg/ml, the crude extract had similar antibacterial effects as gentamicin (0.1 mg/ml) and the ethanol fraction. The 2.5 mg/ml crude ethanol extract of C. aurea suppressed the growth of Pseudomonas aeruginosa, Streptococcus pneumoniae, and Staphylococcus aureus, according to the MIC values. The extract of C. aurea was more effective in inhibiting P. aeruginosa than the other gram-negative bacteria. Fractionation enhanced the antibacterial effect of the extract. All fractionated extracts showed the highest inhibition zone diameter against S. aureus. Petroleum ether extract had the greatest inhibition zone diameter against all bacterial strains. The non-polar components were more active compared to the more polar fractions. The phytochemical components discovered in the leaves of C. aurea included alkaloids, flavonoids, saponins, and tannins. Among these, the tannin content was remarkably high. The current results could provide a rational support for the traditional use of C. aurea to treat skin infections.

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Evaluation of in-vitro antibacterial activity of extracts of Calpurina aurea , Vernonia amygdalina and Rumex nepalensis in Goba district, southeastern Ethiopia

Assefa,

Belay,

Kloos

2024

Egyptian Journal of Basic and Applied Sciences

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Antibacterial activities of Calpurnia aurea and Ocimum lamiifolium extracts against selected gram positive and gram-negative bacteria

Cited by 4 publications

References 7 publications

Ethnobotanical study of traditional medicinal plants used by the local Gamo people in Boreda Abaya District, Gamo Zone, southern Ethiopia

Ethnobotanical study of traditional medicinal plants used by the local Gamo people in Boreda Abaya District, Gamo Zone, southern Ethiopia

Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea

Evaluation of in-vitro antibacterial activity of extracts of Calpurina aurea , Vernonia amygdalina and Rumex nepalensis in Goba district, southeastern Ethiopia

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