Antibacterial activities of Calendula officinalis callus extract

Abstract

The purpose of this study was to determine the antibacterial activity of C. officinalis callus derived from cotyledon explants. Cotyledons excised from in vitro germinated seedlings were used as explants. Explants were transferred on MS medium supplemented with benzil amino purine (BAP; 2 mg l -1 ), α-naphthalene-acetic acid (NAA; 2 mg l -1 ) for callus studies. The cultures were maintained on the same media compositions and were subcultured at an interval of 4 weeks. Callus cultures were harvested at the end of the 16th week. Calli were dried at 40̊ C in the dark for antimicrobial studies. Calendula officinalis callus extracts were tested for their antibacterial activities by using agar well diffusion method. Ethanol and chloroform extracts from these plants were assayed against nine bacteria species (

Suspension Culture of Stem Cells Established from <em>Calendula officinalis </em>L.

“…The coloration of the calli in this study ranged from white to yellow, orange, brown, and black, and the calli were often multicolored. Similar coloration and texture were also found in calli derived from hypocotyls [11], but the calli derived from the leaves and cotyledons were in shades of green [12]. The color of calli induced from leaves and florets also depended on sucrose concentrations in medium and was light green at lower concentrations and cream-yellow at higher concentrations [34].…”

“…The combinations 2.4-D + KIN and 2.4-D + 2iP used previously [12] induced calli with much lower efficiency. Other growth hormones have proven effective for other explants such as hypocotyls, cotyledons, cotyledon nodes [11], and floret explants [34]. Calli initiated from different types of explants of C. officinalis L. tend to be variable in their color and texture, which also changes during cultivation depending on the growth regulators used for callus initiation.…”

“…Callogenesis can also run simultaneously with organogenesis, and then this type of tissue culture can be suitable for the micropropagation of calendula plants [8,35]. Moreover, the long-term cultivation and multiplication of calendula callus in vitro, either unelicited or elicited, is important in the production of useful compounds such as carotenoid pigments [34], salicylic acid [36], and extracts with antibacterial activity [11]. The callus induction stage is already important for the subsequent establishment of cell suspension culture.…”

“…The induction of callus and its growth are fundamental steps for the establishment and applications of other plant tissue cultures. Callus tissues themselves can be used to prepare extracts with properties like antibiotics [11], establish cell suspensions [12], and protoplast Disclaimer/Publisher's Note: The statements, opinions, and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions, or products referred to in the content.…”

Effect of Calendula officinalis Linn in Oral health- A Review

“…The benefits of employing herbs as anti-microbial agents include low toxicity, cost-effectiveness, improved patient tolerance, and the fact that they are renewable in nature (19). Cetin et al found that ethanol and chloroform extracts of C.officinalis callus inhibited Staphylococcus aureus and Bacillus cereus (20). Efstratiou et al revealed that C.officinalis showed excellent antibacterial activity against E. faecalis, E. coli, K. aerogenes, K. pneumonia, and P. aeruginosa (21).…”