Antibacterial activities of Bidens pilosa L, Hoslundia opposita Vahl, and Ageratum conyzoides L against some common wound pathogens

Catherine, Namuga; Haruna, Muwonge; Margaret, Lubwama; Nakyejwe, Janet; Tahalu, Sekulima; John, Baptist Kirabira

doi:10.5897/ajpp2022.5296

Cited by 3 publications

(6 citation statements)

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“…The extracts used in this study were ethyl acetate extract of A. conyzoides extracted by soxhlet and ethanol extract of B. pilosa extracted by Soxhlet extraction. These plants extracts were chosen from preliminary tests done to ascertain their antibacterial activity (Catherine et al, 2022). These extracts showed better antibacterial activity than others thus, were chosen for further assessment.…”

Section: Plant Extractionmentioning

confidence: 99%

“…Apart from treating other illnesses, the plants used in this study are traditionally used for wound healing (Catherine et al, 2022). Hence, the antioxidant activity of these plants justifies their use in treating wounds, where antioxidant activity is crucial for healing.…”

Section: Quantitative Phytochemistry and Antioxidant Activitymentioning

confidence: 99%

“…They contain a plethora of phytochemicals with diverse biological functions, such as anti-inflammatory, antibacterial, anti-diabetic, antiangiogenesis, antiproliferative, and wound healing properties. Plant phytochemicals also serve as valuable sources of antioxidants, a critical attribute in alleviating oxidative stress and promoting wound healing (Catherine et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…conyzoides and B. pilosa are traditionally used in herbal medicine to address various ailments. Scientific research has demonstrated their effectiveness as antimicrobial, anti-inflammatory, antioxidant, antiulcer, and wound healing agents (Catherine et al, 2022). Their antibacterial activity against planktonic bacteria has been studied (Catherine et al, 2022), and their pharmacological properties have been extensively reviewed (Bartolome et al, 2013;Okunade, 2002;Singh et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and antibiofilm activity of Ageratum conyzoides L, and Bidens pilosa L against wound pathogens

Catherine,

Haruna,

Moses

et al. 2024

Afr. J. Pharm. Pharmacol.

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Biofilm infections represent a significant challenge in the medical field due to escalating antibiotic resistance. Plants offer a promising avenue for addressing this issue, as they harbor a diverse array of phytochemicals with various biological activities, including antioxidant properties crucial for preventing biofilm formation in wounds. This study aimed to explore the antioxidant and antibiofilm capabilities of Ageratum conyzoides L. and Bidens pilosa L. The plants underwent quantitative phytochemical screening, antioxidant activity assessment via the DPPH scavenging assay, and antibiofilm activity evaluation using the microtiter plate assay. All plants were found to contain tannins, flavonoids, polyphenols, and alkaloids, with alkaloids being particularly abundant. The antioxidant activity (IC50) of A. conyzoides was measured at 50.52 µg, while that of B. pilosa was 45.94 µg. Regarding the minimum concentration needed to inhibit 50% of Pseudomonas aeruginosa biofilms (MBIC50), it was 24.9 mg/ml for B. pilosa and 28.3 mg/ml for A. conyzoides, with the MBIC90 being 147.7 mg/ml for A. conyzoides and 139.5 mg/ml for B. pilosa. For Staphylococcus aureus biofilms, the MBIC50 was 11.4 mg/ml for B. pilosa and 18.6 mg/ml for A. conyzoides, while the MBIC90 was 69.3 mg/ml for B. pilosa and 97 mg/ml for A. conyzoides. In the biofilm removal assay for P. aeruginosa, the minimum concentration required to eliminate 50% of the formed biofilms (MBEC50) was 38.1 mg/ml for A. conyzoides and 200 mg/ml for B. pilosa. The MBEC90 was 151.7 mg/ml for A. conyzoides and 261 mg/ml for B. pilosa. For S. aureus, the MBEC50 was 96.93 mg/ml for A. conyzoides and 195 mg/ml for B. pilosa, with the MBEC90 being 334 mg/ml and 250 mg/ml for A. conyzoides and B. pilosa, respectively. The study findings confirm that plant extracts possess antioxidant activity and the potential to inhibit biofilm formation and disrupt mature biofilms. The antibiofilm activity observed in these plants underscores their potential as a solution to antibiotic resistance, warranting further research.

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Section: Plant Extractionmentioning

confidence: 99%

Section: Quantitative Phytochemistry and Antioxidant Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant and antibiofilm activity of Ageratum conyzoides L, and Bidens pilosa L against wound pathogens

Catherine,

Haruna,

Moses

et al. 2024

Afr. J. Pharm. Pharmacol.

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“…However, there is scarce information on the antioxidant activity of the specie from Uganda. Additionally, its antibacterial activity has been investigated [ 14 , 20 – 24 ], however, its antibiofilm activity has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing

Namuga,

Muwonge,

Nasifu

et al. 2024

BMC Complement Med Ther

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Background Biofilms and oxidative stress retard wound healing. The resistance of biofilms to antibiotics has led to a search for alternative approaches in biofilm elimination. Antioxidants work synergistically with antibacterial agents against biofilms. Hence recent research has suggested plants as candidates in the development of new alternatives in biofilm treatments and as antioxidants due to the presence of phytocompounds which are responsible for their bioactivities. Hoslundia opposita Vahl is one of the plants used by traditional healers to treat wounds and other infections, this makes it a potential candidate for drug discovery hence, in this study, we investigate the antibiofilm and antioxidant activity of methanolic extract of hoslundia opposita Vahl from Uganda. We also identify phytochemicals responsible for its bioactivity. Method the plant was extracted by maceration using methanol, and the extract was investigated for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. The antibiofilm activity using microtiter plate assay (MTP) assay where the Minimum biofilm inhibitory concentration required to inhibit 50% or 90% of the biofilm (MBIC50 and MBIC90) and Minimum biofilm eradication concentration required to remove 50% or 90% of the biofilm (MBEC50 and MBEC90) were measured. It was further analysed for its phytochemical composition using quantitative screening, as well as Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography mass-spectrometry (LC-MS). Results H. Opposita Vahl extract showed good antioxidant activity with of 249.6 mg/mL. It inhibited the growth of P. aeruginosa and S. aureus biofilms with MBIC50 of 28.37 mg/mL and 10 mg/mL, respectively. It showed the ability to eradicate P. aeruginosa and S. aureus biofilms with MBEC50 of 23.85 and 39.01 mg/mL respectively. Phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, and phenols. GC-MS analysis revealed 122 compounds in the extract of which, 23 have evidence of antioxidant or antibiofilm activity in literature. The most abundant compounds were; 1,4- Citric acid, Tetracontane-1,40-diol (43.43.3%, 1, Olean-12-en-28-oic acid, 3-hydroxy-, methyl ester, (3.beta) (15.36%) 9-Octadecenamide (12.50%), Squalene (11.85%) Palmitic Acid 4TMS (11.28%), and alpha Amyrin (11.27%). The LC-MS identified 115 and 57 compounds in multiple reaction mode (MRM) and scan modes respectively. Conclusion H. opposita Vahl showed antibiofilm and antioxidant activity due to bioactive compounds identified, hence the study justifies its use for wound healing. It can be utilised in further development of new drugs as antibiofilm and antioxidants.

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Ethnobotanical study of medicinal plants used by the people of Mosop, Nandi County in Kenya

Maiyo,

Njeru,

Toroitich

et al. 2024

Front. Pharmacol.

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Background: Throughout the history, nature has provided mankind with most of their basic needs, which include food, shelter, medicine, clothes, flavours, scents as well as raw materials. Given that they are an integral part of cultural heritage, medicinal plants have played a significant role in human healthcare systems around the world. Investigating various biological resources for use as medicines requires ethnomedicinal studies.Methods: Data on utilization of ethnomedicinal plants from local healers in Kenya’s Mosop Sub-County in Nandi County was documented through open-ended, semi-structured questionnaires. A number of quantitative indices, such as the Use Citation (UC), Informant Consensus Factor (ICF), Use Value (UV), Frequency of Citation (FoC) and Relative Frequency of Citation (RFC) were used to convey the potential medical benefits, vitality and variety of the ethnomedicine.Results: 102 informants provided information on 253 ethnomedicinal plant species, classified into 74 families. There were 249 native plant species identified, along with few exotic species: Senegalia senegal (L.) Britton, Persea americana Mill, Carica papaya L. and Solanum betaceum Cav. Of all recorded species, 32% and 27% were herbs and trees, respectively. Among plant parts, leaves were most frequently utilized (27%) and roots (26%), while decoctions (21%) were the most widely used formulations. The dominant family was Asteraceae, with 28 species, followed by Lamiaceae, with 19 species. The highest ICF value was 0.778 for a number of parasitic and infectious illnesses, including ringworms, athlete’s foot rot, tetanus, typhoid, intestinal parasites, abscesses, malaria, and amoebiasis. The study’s data validates the region’s widespread use of traditional medicinal plant remedies.Conclusion: The current study will lay a foundation of knowledge for future research investigations. The abundance of knowledge regarding ethnomedicinal species and their medicinal applications will stimulate further phytochemical and pharmacological research, which could lead to the discovery of potentially significant pharmaceuticals.

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Antibacterial activities of Bidens pilosa L, Hoslundia opposita Vahl, and Ageratum conyzoides L against some common wound pathogens

Cited by 3 publications

References 63 publications

Antioxidant and antibiofilm activity of Ageratum conyzoides L, and Bidens pilosa L against wound pathogens

Antioxidant and antibiofilm activity of Ageratum conyzoides L, and Bidens pilosa L against wound pathogens

Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing

Ethnobotanical study of medicinal plants used by the people of Mosop, Nandi County in Kenya

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