Antibacterial actions of fatty acids and monoglycerides against<i>Helicobacter pylori</i>

Sun, Cynthia Q; O’Connor, Charmian J.; Roberton, Anthony M.

doi:10.1016/s0928-8244(03)00008-7

Cited by 198 publications

(157 citation statements)

References 28 publications

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“…count. Petschow et al (1996) previously showed that the MIC for this fatty acid towards H. pylori was 120 mM and a bactericidal effect was observed at 1.0 mM (Sun et al, 2003). However, the effective bactericidal concentration of LA during live/dead analysis in the current study was v32 mM.…”

Section: Discussioncontrasting

confidence: 61%

“…This protective mechanism of BSA was also described by Hazell & Graham (1990). The fatty acids showed antibacterial activity (Nieman, 1954;Kabara et al, 1972;Knapp & Melly, 1986), and several studies established that commercially available medium-chain fatty acids and monoglycerides could inhibit the growth of H. pylori (Petschow et al, 1996;Sun et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Identification of self-growth-inhibiting compounds lauric acid and 7-(Z)-tetradecenoic acid from Helicobacter pylori

et al. 2015

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Helicobacter pylori growth medium is usually supplemented with horse serum (HS) or FCS. However, cyclodextrin derivatives or activated charcoal can replace serum. In this study, we purified self-growth-inhibiting (SGI) compounds from H. pylori growth medium. The compounds were recovered from porous resin, Diaion HP-20, which was added to the H. pylori growth medium instead of known supplements. These SGI compounds were also identified from 2,6-di-O-methyl-b-cyclodextrin, which was supplemented in a pleuropneumonia-like organisms broth. The growth-inhibiting compounds were identified as lauric acid (LA) and 7-(Z)-tetradecenoic acid [7-(Z)-TDA]. Although several fatty acids had been identified in H. pylori, these specific compounds were not previously found in this species. However, we confirmed that these fatty acids were universally present in the cultivation medium of the H. pylori strains examined in this study. A live/dead assay carried out without HS indicated that these compounds were bacteriostatic; however, no significant growth-inhibiting effect was observed against other tested bacterial species that constituted the indigenous bacterial flora. These findings suggested that LA and 7-(Z)-TDA might play important roles in the survival of H. pylori in human stomach epithelial cells.

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Section: Discussioncontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Identification of self-growth-inhibiting compounds lauric acid and 7-(Z)-tetradecenoic acid from Helicobacter pylori

et al. 2015

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“…Generally, Gram-positive bacteria are more sensitive whereas Gram-negative bacteria are not (Sprong et al 2001). This phenomenon may be caused by the difference between the outer membrane of the cell wall of Gram-positive and Gram-negative bacteria (Sun et al 2003). Recently, however, some lipid-sensitive Gram-negative bacteria have been described.…”

mentioning

confidence: 99%

Antimicrobial effects of fatty acid fructose esters

Karlová¹,

Poláková²,

Šmidrkal³

et al. 2010

Czech J. Food Sci.

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Karlová T., Poláková L., Šmidrkal J., Filip V. (2010): Antimicrobial effects of fatty acid fructose esters. Czech J. Food Sci., 28: 146-149.Antimicrobial effects of various fatty acids and their esters have been extensively studied. Esters with saccharides (glucose, sucrose) have been found to have a broad spectrum of microbicidal activity. The objective of this study was to investigate the susceptibility of four microbial strains (Bacillus cereus, Escherichia coli, Saccharomyces cerevisiae, and Fusarium culmorum) to the antimicrobial properties of fatty acid (capric, lauric, myristic, and palmitic) fructose esters. Microorganisms were cultivated in liquid media supplemented with various concentrations of the tested agents. A spectrophotometric method was used for the quantitative detection of the microbial growth. Both the cultivation and measuring of the absorbance was carried out in microtiter plates. Our results indicate that the addition of the tested compounds strongly reduce the number of viable microorganisms. Higher concentrations caused microbicidal effect. The inhibitory action decreased rapidly as the chain length increased. Caprinoylfructose proved to be the most active.

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“…For instance, Marounek et al (2003) found no effect on the viable E. coli count after short term incubation with C8 or C10 at pH 6.5 but a strong decrease was observed at a pH of 5.2. Furthermore, Sun et al (2003) found that even at a concentration of 1 mM C8, bactericidal effects were visible between pH 3.5 and pH 5.5. However, Petschow et al (1996) testing the bactericidal activity of C12 against H. pylori did not find differences using pH ranging from 3 to 7.…”

Section: Discussionmentioning

confidence: 98%

Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells

2016

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Medium-chain fatty acids (MCFAs) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. First, we studied the sensitivity of Salmonella Enteritidis and an enteropathogenic Escherichia coli strain against caprylic (C8), capric (C10) and lauric (C12) acids. A porcine in vitro model using the porcine cell line IPEC-J2 was used to test the effects of MCFAs on structural and immunological traits without and with a concomitant challenge with E. coli or S. Enteritidis. The three MCFAs exerted an inhibitory effect on bacterial growth, stronger for C12 than C8 or C10, S. Enteritidis being more sensitive than the E. coli strain. Flow cytometry showed a numeric concentration dependent increase in the adhesion of E. coli or S. Enteritidis to IPEC-J2 cells. Measurement of transepithelial electrical resistance after bacterial challenge showed negative effects of all MCFAs on IPEC-J2 cells at the highest concentrations. Immune parameters were affected by C8, since a concentration dependent effect starting at 5 mM was observed for mRNA expression of IL-6 and TLR-4 (up-regulated) and IL-8 (down-regulated). TLR-4 was up-regulated with C10 at 2 and 5 mM. The three MCFAs affected also the epithelial morphology through downregulation of Occludin and up-regulation of Claudin-4 expression. In conclusion, the three MCFAs under study influenced bacterial growth rates and modified the gene expression to a different degree in the cell line IPEC-J2 but the effect on the morphological structure and response of the cells after bacterial challenge could not be assessed. Although these tests show a prior estimation of MCFAs effects in intestinal epithelium, in vivo confirmation is still needed.

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Antibacterial actions of fatty acids and monoglycerides againstHelicobacter pylori

Cited by 198 publications

References 28 publications

Identification of self-growth-inhibiting compounds lauric acid and 7-(Z)-tetradecenoic acid from Helicobacter pylori

Identification of self-growth-inhibiting compounds lauric acid and 7-(Z)-tetradecenoic acid from Helicobacter pylori

Antimicrobial effects of fatty acid fructose esters

Effects of medium-chain fatty acids on the structure and immune response of IPEC-J2 cells

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