Antibacterial 3D-Printed Silver Nanoparticle/Poly Lactic-Co-Glycolic Acid (PLGA) Scaffolds for Bone Tissue Engineering

Chen, Fajun; Han, Jian; Guo, Zhenqiu; Chongjing, Mu; Yu, Chuandi; Ji, Zhibo; Sun, Lei; Wang, Yujuan; Wang, Junfeng

doi:10.3390/ma16113895

Cited by 15 publications

(7 citation statements)

References 62 publications

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“…The results of elemental analysis using EDAX also reveals that the PCL/BG/ZnO and PCL/BG/GO systems show higher calcium phosphate deposition compared with other composite scaffolds. AgNPs enhance scaffold bioactivity, preventing bacterial infections, stimulating osteogenesis, and promoting angiogenesis. − AgNPs scaffold enhance bone tissue engineering with improved osteogenesis and antibacterial properties. − Controlling the Ag NP concentration and release rate is crucial to avoid cytotoxic effects. Long-term evaluations are necessary for safe and effective use in various tissue engineering applications.…”

Section: Resultsmentioning

confidence: 99%

Enhancing Bioactivity of Nanofibrous Poly(Caprolactone)/45S5 Bioglass Composite Scaffolds by Incorporation of Ag, GO, and ZnO Nanoparticles

Ramar,

Bensingh,

Bhuvana

2023

ACS Biomater. Sci. Eng.

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The present study endeavors toward the investigation on the bioactivity of nanofibrous scaffolds manufactured by the electrospinning process. Nanofibrous composite scaffolds of PCL with 45S5 bioactive glass and metal oxide nanoparticles were developed and characterized. The effects of incorporating silver (Ag), graphene oxide (GO), and zinc oxide (ZnO) nanoparticles into PCL/bioglass nanofibrous scaffolds on its geometry and physiochemical, morphological, mechanical, and biological properties were studied. The incorporation of GO and ZnO alters the fiber diameter, suggesting the methodology for controlling the porosity of the scaffolds. The results of FTIR and XRD confirm the structure of bioglass, Ag, GO and ZnO nanoparticles. The in vitro degradation studies in SBF solution provide evidence for the enhancement in the rate of apatite formation by the inclusion of nanoparticles as compared with PCL/BG scaffolds. The assessment of mechanical properties suggests the tensile strength was increased from 1.61 to 5 MPa in PCL/BG/ZnO system when compared with pristine PCL. The cell viability is also observed to be improved from 72% to 91% and 104% for PCL/BG/GO and PCL/BG/ZnO, respectively. The hemolytic activity studies confirm that all scaffolds are nonhemolytic in nature and PCL/BG/ZnO exhibits the least hemolytic activity of 0.65% among the other composite scaffolds, suggesting the better blood compatibility. The present study evidently shows the fact that incorporation of GO and ZnO nanoparticles with PCL in addition to BG accelerates the bioactivity and improves the mechanical strength of the scaffold.

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Section: Resultsmentioning

confidence: 99%

Enhancing Bioactivity of Nanofibrous Poly(Caprolactone)/45S5 Bioglass Composite Scaffolds by Incorporation of Ag, GO, and ZnO Nanoparticles

Ramar,

Bensingh,

Bhuvana

2023

ACS Biomater. Sci. Eng.

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“…The 3D-printing process was easy to set up and fast to carry out, taking 7 min to print a scaffold with dimensions equivalent to those of the average ACL. This process yielded reproducible samples with high structural homogeneity and controlled geometry, indicating that the printing parameters could be easily adjusted according to the shape and location of the injured tissue [ 43 ]. Figure 2 illustrates the 3D-printed PLA (a1–a4) and PLA+0.5[(f-EG)+Ag] scaffolds (b1–b4), at the top, front, and lateral views, at increasing magnifications of their porous morphology.…”

Section: Resultsmentioning

confidence: 99%

Biocompatible 3D-Printed Tendon/Ligament Scaffolds Based on Polylactic Acid/Graphite Nanoplatelet Composites

Silva,

Gomes,

Correia

et al. 2023

Nanomaterials

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Three-dimensional (3D) printing technology has become a popular tool to produce complex structures. It has great potential in the regenerative medicine field to produce customizable and reproducible scaffolds with high control of dimensions and porosity. This study was focused on the investigation of new biocompatible and biodegradable 3D-printed scaffolds with suitable mechanical properties to assist tendon and ligament regeneration. Polylactic acid (PLA) scaffolds were reinforced with 0.5 wt.% of functionalized graphite nanoplatelets decorated with silver nanoparticles ((f-EG)+Ag). The functionalization of graphene was carried out to strengthen the interface with the polymer. (f-EG)+Ag exhibited antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), an important feature for the healing process and prevention of bacterial infections. The scaffolds’ structure, biodegradation, and mechanical properties were assessed to confirm their suitability for tendon and ligamentregeneration. All scaffolds exhibited surface nanoroughness created during printing, which was increased by the filler presence. The wet state dynamic mechanical analysis proved that the incorporation of reinforcement led to an increase in the storage modulus, compared with neat PLA. The cytotoxicity assays using L929 fibroblasts showed that the scaffolds were biocompatible. The PLA+[(f-EG)+Ag] scaffolds were also loaded with human tendon-derived cells and showed their capability to maintain the tenogenic commitment with an increase in the gene expression of specific tendon/ligament-related markers. The results demonstrate the potential application of these new 3D-printed nanocomposite scaffolds for tendon and ligament regeneration.

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“…For example, a study used gelatin-based scaffolds loaded with chitosan nanoparticles to deliver vascular endothelial growth factor (VEGF) in order to promote angiogenesis in wound healing [244]. Similarly, 3D-printed polylactic acid (PLA) scaffolds with the addition of polyethyleneimine-coated iron oxide nanoparticles were used to encapsulate and control the release of bone morphogenetic protein-2 (BMP-2), a growth factor that promotes bone regeneration [245]. iii Biomimicry: Three-dimensionally printed polymers with nanoparticles can be designed to mimic the structure and properties of natural tissues.…”

Section: Regeneration Applicationsmentioning

confidence: 99%

Promising New Horizons in Medicine: Medical Advancements with Nanocomposite Manufacturing via 3D Printing

Li,

Khan,

Chen

et al. 2023

Polymers

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Three-dimensional printing technology has fundamentally revolutionized the product development processes in several industries. Three-dimensional printing enables the creation of tailored prostheses and other medical equipment, anatomical models for surgical planning and training, and even innovative means of directly giving drugs to patients. Polymers and their composites have found broad usage in the healthcare business due to their many beneficial properties. As a result, the application of 3D printing technology in the medical area has transformed the design and manufacturing of medical devices and prosthetics. Polymers and their composites have become attractive materials in this industry because of their unique mechanical, thermal, electrical, and optical qualities. This review article presents a comprehensive analysis of the current state-of-the-art applications of polymer and its composites in the medical field using 3D printing technology. It covers the latest research developments in the design and manufacturing of patient-specific medical devices, prostheses, and anatomical models for surgical planning and training. The article also discusses the use of 3D printing technology for drug delivery systems (DDS) and tissue engineering. Various 3D printing techniques, such as stereolithography, fused deposition modeling (FDM), and selective laser sintering (SLS), are reviewed, along with their benefits and drawbacks. Legal and regulatory issues related to the use of 3D printing technology in the medical field are also addressed. The article concludes with an outlook on the future potential of polymer and its composites in 3D printing technology for the medical field. The research findings indicate that 3D printing technology has enormous potential to revolutionize the development and manufacture of medical devices, leading to improved patient outcomes and better healthcare services.

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Antibacterial 3D-Printed Silver Nanoparticle/Poly Lactic-Co-Glycolic Acid (PLGA) Scaffolds for Bone Tissue Engineering

Cited by 15 publications

References 62 publications

Enhancing Bioactivity of Nanofibrous Poly(Caprolactone)/45S5 Bioglass Composite Scaffolds by Incorporation of Ag, GO, and ZnO Nanoparticles

Enhancing Bioactivity of Nanofibrous Poly(Caprolactone)/45S5 Bioglass Composite Scaffolds by Incorporation of Ag, GO, and ZnO Nanoparticles

Biocompatible 3D-Printed Tendon/Ligament Scaffolds Based on Polylactic Acid/Graphite Nanoplatelet Composites

Promising New Horizons in Medicine: Medical Advancements with Nanocomposite Manufacturing via 3D Printing

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