Antiapoptotic and embryotrophic effects of α-tocopherol and l-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer

Jeong, Yeon Woo; Park, Sun Woo; Hossein, Mohammad Shamim; Kim, Sue; Kim, Ji Hye; Lee, So Hyun; Kang, Sung Keun; Lee, Byeong Chun; Hwang, Woo Suk

doi:10.1016/j.theriogenology.2006.06.007

Cited by 54 publications

(46 citation statements)

References 35 publications

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“…Beyond this concentration, a severe decrease of blastocyst yield was observed. Dalvit et al (1998) and Jeong et al (2006) both corroborate our results, reporting that supplementing the medium with 100 µM of α-tocopherol added to 100 µM of L-ascorbic acid was detrimental, perhaps due to a higher concentration of antioxidants, which, in that case, would be 200 µM, as these two antioxidants have a cumulative effect, provoking toxicity in embryos. In fact, the presence of ROS in the culture media for extended periods of time and with high dosages, results in embryo toxicity (Wang et al 2002) and may impact post-fertilization development to the blastocyst stage and embryo quality, which are common indicators of assisted reproduction outcomes (Agarwal et al 2005).…”

Section: )supporting

confidence: 87%

Effect of α-tocopherol on in vitro culture of bovine embryos

Marques

Antunes²,

Santos³

et al. 2007

Can. J. Anim. Sci.

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. 2007. Effect of α α-tocopherol on in vitro culture of bovine embryos. Can. J. Anim. Sci. 87: 539-542. Bovine oocytes were matured and fertilised under 5% CO 2 . Presumptive zygotes were co-cultured in synthetic oviductal fluid droplets, supplemented with either 0 (control), 25, 50, 100, or 200 µM of α-tocopherol. Blastocyst development rates were significantly influenced (P < 0.05) by the level of antioxidant in the culture media. Control showed a blastocyst yield of 18.46, 21.11, 27.92, and 31.66%, respectively, at α25; α50 and α100. Blastocyst yield for α200 was severely decreased, to 8.01%. An increase in overall IVF results was also observed as the concentration of α-tocopherol increased (control, 14.21%; α25, 14.35%; α50, 19.52%; α100, 21.11%), decreasing to 5.75% for the concentration of α200. The present study clearly demonstrates that α-tocopherol at a concentration of 100 µM significantly improves the proportion of oocytes that develop to the blastocyst stage.Key words: Antioxidant, α-tocopherol, reactive oxygen species, bovine, embryo Marques, A., Antunes, G., Santos, P., Chaveiro, A. et da Silva, F. M. 2007. Incidence du α α-tocophérol sur la culture in vitro des embryons de bovins. Can. J. Anim. Sci. 87: 539-542. Des ovocytes de bovins ont été amenés à maturité puis fécondés dans une atmosphère à 5 % de CO 2 . Les zygotes présomptifs ont été cultivés ensemble dans des gouttelettes de fluide synthétique de l'oviducte enrichi avec 0 µM (témoin), 25 µM, 50 µM, 100 µM ou 200 µM de α-tocophérol. La concentration d'antioxydant dans le milieu influe sensiblement (P < 0,05) sur le développement des blastocystes. Les témoins affichent un rendement en blastocystes de 18,46 % contre 21,11 %, 27,92 % et 31,66 %, respectivement, pour les milieux α25, α50 et α100. Le milieu α200 présentait une forte diminution de la proportion de blastocystes (8,01 %). La hausse de la concentration de α-tocophérol entraîne une amélio-ration générale de la fécondation in vitro (témoin-14,21 %; α25-14,35 %; α50-19,52 %; α100-21,11 %), qui tombe à 5,75 % à la concentration α200. L'étude montre clairement qu'une concentration de 100 µM de α-tocophérol améliore sensiblement la proportion d'ovocytes qui parviennent au stade du blastocyste.

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Section: )supporting

confidence: 87%

Effect of α-tocopherol on in vitro culture of bovine embryos

Marques

Antunes²,

Santos³

et al. 2007

Can. J. Anim. Sci.

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“…In addition, oxidative stress by reactive oxygen species (ROS) must be one of the causes that may induce lipid peroxidation and/or organelle damage in bovine oocytes (Gutnisky et al 2013). Mechanism of radical scavenger might be involved in the effect of antioxidants (L-ascorbic acid and a-tocopherol) that can improve the blastocyst yield from IVM/IVF oocytes in cattle (Olson & Seidel 2000) and pigs (Kitagawa et al 2004, Jeong et al 2006, Hossein et al 2007.…”

Section: Introductionmentioning

confidence: 99%

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

et al. 2015

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The objective of this study was to investigate whether developmental competence of vitrified-warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either L-ascorbic acid or a-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to a-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1-36.3% vs 19.2-25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between a-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in a-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with a-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that a-tocopherol treatment of vitrified-warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.

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“…Earlier studies in porcine indicate that the blastocyst quality of in vitro fertilized and somatic cell nuclear transferred embryos was improved when embryo culture media was supplemented with α-tocopherol [14]. Studies on bovine suggest that culture of embryos with vitamin E resulted in development of more numbers of embryos to early and expanded blastocysts than that of the control group [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of α-tocopherol supplementation on in vitro maturation of sheep oocytes and in vitro development of preimplantation sheep embryos to the blastocyst stage

Natarajan

Shankar²,

Deecaraman

2010

J Assist Reprod Genet

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Purpose To determine the effects of α-tocopherol supplementation to oocyte maturation media and embryo culture media on the yield of ovine embryos. Methods α-tocopherol, at concentrations of 0, 50, 100, 200, 400 and 500 µM was supplemented to ovine oocyte or embryo culture media and cultured at 5% or 20% O 2 levels. Percentages of cleavage, morula and blastocyst, total cell count and comet assay were taken as indicators of developmental competence of embryos. Results 200 µM α-tocopherol in embryo culture medium at 20% O 2 level significantly increased the rates of cleavage (P<0.05), morulae (P<0.05) and blastocyst (P<0.01) formation and blastocyst total cell number (P<0.01) when compared with control. The rates of blastocyst formation were also significantly higher in 100 µM (P<0.01) and 400 µM (P<0.05) supplemented groups than control. Conclusion α-tocopherol supplementation may enhance the in vitro developmental competence of ovine embryos by protecting them from oxidative damage.

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Antiapoptotic and embryotrophic effects of α-tocopherol and l-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer

Cited by 54 publications

References 35 publications

Effect of α-tocopherol on in vitro culture of bovine embryos

Effect of α-tocopherol on in vitro culture of bovine embryos

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

Effect of α-tocopherol supplementation on in vitro maturation of sheep oocytes and in vitro development of preimplantation sheep embryos to the blastocyst stage

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