Antiangiogenic and antitumoral properties of a polysaccharide isolated from the seaweed Sargassum stenophyllum

Dias, Paulo Fernando; Siqueira, Jarbas Mota; Vendruscolo, Luiz Felipe; Neiva, Teresinha de Jesus Carvalho; Gagliardi, Antônio; Maraschin, Marcelo; Ribeiro‐do‐Valle, Rosa Maria

doi:10.1007/s00280-004-0995-7

Cited by 84 publications

(38 citation statements)

References 68 publications

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“…To evaluate the activity of thalidomide and its synthetic analogues (2a and 2b) on the blood vessels' formation, the chorioallantoic membrane (CAM) assay was used [25,26]. Briefly, eggs were incubated in a hatcher at 38.5°C under a humidified atmosphere (33% relative humidity) and with rotary movement every 2 hours.…”

Section: In Vivo Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Costa

Carvalho

et al. 2015

Chemico-Biological Interactions

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The strategy of antiangiogenic drugs is based on inhibiting formation of new blood vessels as alternative to limit cancer progression. In this work, we investigated the antitumor and antiangiogenic potential of eight thalidomide derivatives. Most of the molecules was not cytotoxic but 2a, 2d and 3d revealed weak antiproliferative activity on HL-60, Sarcoma 180 (S180) and normal peripheral blood mononuclear cells. Thalidomide, 2a and 2b were able to inhibit tumor growth (53.5%, 67.9% and 67.4%, respectively) in S180-bearing mice and presented moderate and reversible toxicity on liver, kidneys and spleens. Both analogs (2a and 2b) inhibited cell migration of endothelial (HUVEC) and melanoma cells (MDA/MB-435) at 50μg/mL. Immunohistochemistry labeling assays with CD-31 (PECAM-1) antibody showed microvascular density (MVD) was significantly reduced in thalidomide, 2a and 2b groups (30±4.9, 64.6±1.8 and 46.5±19.5%, respectively) (p<0.05). Neovascularization evaluated by Chorioallantoic Membrane Assay (CAM) with compounds 2a and 2b showed reduction of vessels' number (12. 9±2.3 and 14.8±3.3%), neovascularization area (13.1±1.7 and 14.3±1.7%) and total length of vessels (9.2±1.5 and 9.9±1.9%). On the other hand, thalidomide did not alter vascularization parameters. Consequently, addition of thiosemicarbazone pharmacophore group into the phthalimidic ring improved the in vivo antitumor and antiangiogenic potential of the analogs 2a and 2b.

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Section: In Vivo Chorioallantoic Membrane (Cam) Assaymentioning

confidence: 99%

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Costa

Carvalho

et al. 2015

Chemico-Biological Interactions

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“…Polysaccharides isolated from algae are known to exhibit various biological activities such as antitumor, antiviral, anti-inflammatory, antiangiogenic, and immunostimulating. Thus, water-soluble polysaccharides and alginates isolated from algae of the genus Sargassum exhibited antitumor properties [16][17][18][19][20]; sulfonated polysaccharides, antiviral properties [6,[21][22][23].…”

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confidence: 99%

Composition, structural characteristics, and antitumor properties of polysaccharides from the brown algae Dictyopteris polypodioides and Sargassum sp.

et al. 2011

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The polysaccharide compositions of the brown algae Dictyopteris polypodioides and Sargassum sp. from the Mediterranean Sea were determined. The principal polysaccharide of the studied algae (about 12% of the dry alga weight) was alginic acid. The content of water-soluble polysaccharides was low. The amount of fucoidan was less than 1% of the dry alga weight; of neutral polysaccharides, less than 0.25%. The monosaccharide compositions of fucoidans and neutral polysaccharides were investigated. Experiments on soft agar-agar models showed that fucoidans from D. polypodioides and Sargassum sp. exhibited antitumor activity against RPMI-7951 human melanoma cells.The search for biologically active compounds from natural sources is currently of great interest. Polysaccharide-type compounds exhibit a broad spectrum of biological activity and low in vivo toxicity.Brown algae contain compounds with structures that differ from those in red and green algae and terrestrial plants. Mannitol and comparatively low-molecular-weight 1,3;1,6-E-D-glucans (laminarans) act as reserve material in them [1]. Salts of alginic acids (alginates) [2] and complex sulfonated polysaccharides (fucoidans) [3,4] occur in cell walls and intercellular space. The latter exhibit a broad spectrum of biological activity. Structure-activity relationships of the polysaccharides are rather difficult to establish because their structures are heterogeneous, irregular, branched, and highly sulfonated.The goal of the present work was to study the composition, structural characteristics, and antitumor activity of polysaccharides from the Mediterranean algae Dictyopteris polypodioides and Sargassum sp.Alginic acids, neutral polysaccharides, and fucoidans were isolated from these brown algae. Dried and ground algae were treated with EtOH (70%) to remove low-molecular-weight compounds and then extracted with HCl solution. The extract containing water-soluble polysaccharides was concentrated, dialyzed, and placed on a column with the hydrophobic sorbent Polychrom-1. Charged polysaccharides (Dp-F and S-F) were eluted by H 2 O; neutral polysaccharides, by aqueous EtOH (5 and 15%) to afford fractions Dp-5%, Dp-15%, S-5%, and S-15%. Remaining algae were extracted by Na 2 CO 3 solution. The extracts were dialyzed. Alginic acid was precipitated by three times the volume of EtOH (96%) to afford Dp-A and S-A. Fractions Dp-F and S-F contained fucoidans after Polychrom-1 and were separated over a column of Macro-Prep DEAE to afford Dp-F1, Dp-F2, Dp-F3, and Dp-F4 and S-F1, S-F2, S-F3, and S-F4, which were lyophilized to produce alginic acids, neutral polysaccharides, and fucoidans. Table 1 presents the yields of polysaccharides. Table 1 shows that the principal polysaccharide from the studied algae was alginic acid (greater than 12% for both algae), the content of which depended on the species, season, latitude, and ecological habitat [5]. According to the literature, the content of alginic acids in algae of the genus Sargassum varies from 3 to 17% [6,7]. The studied alga coul...

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“…These are primitive nonflowering plants, which are the good source of food and medicine (Kaliaperumal, 2003). Various species of Sargassum (Sargassaceae) have been found to possess antiviral, anticancer and antitumor activities (Dias et al, 2005). Farnasylacetone derivatives obtained from S. sagamianum Yendo have been found to possess anticholinesterase activity (Ryu et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and anti-cholinesterase activity ofSargassum wightii

Syad

Shunmugiah

Kasi

2013

Pharmaceutical Biology

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Context: Sargassum has been used in traditional Chinese medicine since the eighth century AD to treat goiter. Sargassum wightii Greville (Sargassaceae) is a major source of alginic acid used widely in food and drug industries. Objective: To evaluate the anti-Alzheimer potential of S. wightii through evaluation of antioxidant and cholinesterase inhibitory activities. Materials and methods: Successive extraction was done using solvents of varying polarity. Solvent extracts (100-500 mg/mL) were employed for all the antioxidant assays. Free radical scavenging activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl, OH. , H 2 O 2 radical scavenging assay. The reducing power of the seaweed was evaluated by ferric reducing antioxidant power and reducing power assay. Cholinesterase (ChE) inhibitory activity was evaluated and the Km, Vmax and Ki were calculated. Further, compound characterization was done by GC-MS analysis. Results: The non-polar extracts were found to possess significant antioxidant activity. At 100 mg/mL, petroleum ether, hexane, benzene and dichloromethane extracts showed significant ChE inhibitory activity with IC 50 values of 19.33 AE 0.56, 46.81 AE 1.62, 27.24 AE 0.90, 50.56 AE 0.90 mg/mL, respectively, for AChE, and 17.91 AE 0.65, 32.75 AE 1.00, 12.98 AE 0.31, 36.16 AE 0.64 mg/mL, respectively, for BuChE. GC-MS reveals that 1,2-benzenedicarboxylic acid, diisooctyl ester is the major compound present in dichloromethane extract of S. wightii. The mode of inhibition exhibited by dichloromethane extract against the cholinesterases was found to be competitive type. Discussion and conclusion: The presence of high amount of terpenoids could be the possible reason for its potential antioxidant and ChE inhibitory activity.

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Antiangiogenic and antitumoral properties of a polysaccharide isolated from the seaweed Sargassum stenophyllum

Cited by 84 publications

References 68 publications

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Improvement of in vivo anticancer and antiangiogenic potential of thalidomide derivatives

Composition, structural characteristics, and antitumor properties of polysaccharides from the brown algae Dictyopteris polypodioides and Sargassum sp.

Antioxidant and anti-cholinesterase activity ofSargassum wightii

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