Antiangiogenic Activity of Compounds Isolated from Anarrhinum pedatum

Beladjila, Khadidja Aya; Berrehal, Djemaa; Kabouche, Zahia; Germanò, Maria Paola; D’Angelo, Valeria; Tommasi, Nunziatina De; D’Andrea, Felicia; Braca, Alessandra; Leo, Marinella De

doi:10.1021/acs.jnatprod.8b00893

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…These iridoid derivatives isolated from N. reticulata induced cytotoxic and anti-inflammatory activities on hepatocarcinoma cells and the macrophage cell line, respectively [43][44][45]. The iridoid glycosides isolated from other plant species were also found to have an anti-angiogenic property and can suppress tumour migration and invasion [46][47][48].…”

Section: Discussionmentioning

confidence: 98%

Neonauclea formicaria (Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos

Vergara

Demetillo

Ombat

et al. 2021

ILNS

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Plants are reservoirs of bioactive compounds with the potential for pharmaceutical use. In this study, the secondary metabolites of Neonauclea formicaria leaf crude ethanolic extract were determined using phytochemical screening. The plant's leaf extract was then used to test its angiogenesis activity using the chorioallantoic membrane (CAM) assay. Four concentrations of the extract were prepared—0.1 mg/L, 1.0 mg/L, 10.0 mg/L, and 100.0 mg/L and were topically applied on the CAM. Phytochemical screening revealed that N. formicaria leaves contain heavy amounts of flavonoids and tannins, while alkaloids, saponins, and steroids were present in trace amounts. The crude ethanolic extract was anti-angiogenic, as indicated by the significant decrease of vascular density at higher concentrations (P<0.05). The 100 mg/L extract concentration showed the highest vascular inhibition (50.93%) among the other concentrations, suggesting its angiopreventive potential (P<0.05). Further investigation on the embryo's gross morphometry revealed no significant effects in the weight, crown-rump length, head-beak length, forelimb length, and hind limb length. Also, these indices were not associated with the angiogenesis activity on the CAM. Further studies exploring the specific metabolites of the different plant parts of N. formicaria and the plant's angiopreventive potential are recommended.

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Section: Discussionmentioning

confidence: 98%

Neonauclea formicaria (Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos

Vergara

Demetillo

Ombat

et al. 2021

ILNS

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“…It has been found that compared with human umbilical vein endothelial cell and chicken embryo allantoic membrane models, the zebrafish model is more simplistic and practical, making it suitable for the screening of active ingredients in traditional Chinese medicine that exhibit antiangiogenic properties. In addition, the angiogenesis process of zebrafish embryos is very similar to that of malignant tumors ( 14 , 15 ). Therefore, in this study, we established a transgenic zebrafish model, investigated and observed the blood vessels of transgenic zebrafish using fluorescent labeling, and counted the intersegmental vessels (ISVs) to explore the inhibitory effect of tryptanthrin on angiogenesis.…”

Section: Introductionmentioning

confidence: 95%

Tryptanthrin inhibits tumor angiogenesis via Notch/Dll4 signaling pathway in zebrafish

Lin,

Dai,

Zheng

et al. 2023

Transl Cancer Res

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Background Anti-angiogenic pathways are important for inhibiting tumor growth and migration. Tryptanthrin has anticancer properties in vivo but its anti-angiogenesis activities and associated mechanisms remain unclear. Methods The effects of tryptanthrin were investigated in vivo using fluorescent labeling of blood vessels in zebrafish. Fluorescence quantitation was conducted to analyze the level of delta-like ligand 4 ( Dll4 ) gene expression. Transcriptome sequencing and quantitative polymerase chain reaction (qPCR) analyses were performed to explore the molecular mechanisms of anti-tumor angiogenesis. Results Significant anti-tumor effects were observed in all 48-hpf (hours post-fertilization) zebrafish treated with tryptanthrin (P<0.05). The 6-hpf zebrafish were cultured to 48 and 72 hpf following tryptanthrin treatment. It was found that compared with the control groups, the fluorescence area and the number of complete internode vessels reduced significantly following treatment with medium and high concentrations of tryptanthrin (P<0.05). The relative expression of Dll4 in the 48-hpf zebrafish was significantly inhibited only in the high concentration group (P<0.05). qPCR analysis revealed that the levels of Krt18b , desma , Tnnt2c , and Krt4 gene expression were significantly up-regulated in zebrafish following Dll4 overexpression. After Dll4 knockdown, the level of desma and Tnnt2c gene expression was significantly up-regulated. Conclusions Tryptanthrin can inhibit tumor growth in vivo in a concentration-dependent manner by down-regulating Dll4 protein expression, and at the same time up-regulating the level of desma and Tnnt2c gene expression.

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“…In this paper, the isolation of one new iridoid glucoside, 6βacetoxyipolamiide (1), and thirteen (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) known congeners from two Lamiaceae species, Stachys ocymastrum and Premna resinosa, leaf extracts is reported. The structural determination of the isolated compounds was performed by monoand bidimensional NMR spectroscopic analysis as well as MS experiments.…”

Section: Introductionmentioning

confidence: 99%

Antiangiogenic Iridoids from Stachys ocymastrum and Premna resinosa

et al. 2019

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In this paper, the isolation of one new iridoid glucoside, 6β-acetoxyipolamiide (1), and thirteen (2 – 14) known congeners from two Lamiaceae species, Stachys ocymastrum and Premna resinosa, leaf extracts is reported. The structural determination of the isolated compounds was performed by mono- and bidimensional NMR spectroscopic analysis as well as MS experiments. The isolates were assayed for their antiangiogenic activity by two in vivo models, zebrafish embryos and chick chorioallantoic membrane assays. The compounds with a significant antiangiogenic activity in both assays were β-hydroxyipolamiide (2), ipolamiide (3), and buddlejoside A5 (8). 6-O-α-l-(3″-O-p-Methoxycinnamoyl-4″-O-acetyl)rhamnopyranosyl catalpol (13) and 6-O-α-l-(2″-trans-caffeoyl)rhamnopyranosyl catalpol (6) showed the best antiangiogenic response on blood vessel growth in zebrafish embryos, whereas saccatoside (10) and 6-O-α-l-(2″-O - p-methoxycinnamoyl-3″-O-acetyl)rhamnopyranosyl catalpol (14) resulted in a strong reduction of capillary formation in the chorioallantoic membrane assay.

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Antiangiogenic Activity of Compounds Isolated from Anarrhinum pedatum

Cited by 10 publications

References 22 publications

<i>Neonauclea </i><i>formicaria</i><i> </i>(Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos<i></i>

<i>Neonauclea </i><i>formicaria</i><i> </i>(Rubiaceae) Leaf Extract Inhibits Vascularization in the Chorioallantoic Membrane of Duck Embryos<i></i>

Tryptanthrin inhibits tumor angiogenesis via Notch/Dll4 signaling pathway in zebrafish

Antiangiogenic Iridoids from Stachys ocymastrum and Premna resinosa

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