Antiandrogenic Pesticides Disrupt Sexual Characteristics in the Adult Male Guppy (Poecilia reticulata)

Baatrup, Erik; Junge, Mette

doi:10.2307/3454962

Cited by 43 publications

(61 citation statements)

References 46 publications

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“…This reduction in AR expression appears specific to the mesenchyme immediately adjacent to the urethral epithelium, and suggests a role for Hoxa13 in mediating the responsiveness of these tissues to androgens by modulating the levels of AR in the urethral epithelium. This modulation in AR expression would provide a developmental link between hypospadias associated with environmental antagonists of the AR and hypospadias associated with loss of Hoxa13 function, placing Hoxa13 as an important modulator of AR signaling during the development of the external genitalia (Baatrup and Junge, 2001;Curtis, 2001;Baskin et al, 2001). Alternatively, environmental compounds could affect Hoxa13 expression directly, in a manner similar to the ectopic expression of Hox genes in response to exogenous retinoids (Simeone et al, 1991;Whiting, 1997).…”

Section: Hoxa13 Is Essential For Maintaining Capillary Vessel Diametementioning

confidence: 99%

Loss of Bmp7 and Fgf8 signaling in Hoxa13-mutant mice causes hypospadia

et al. 2003

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In humans and mice, mutations in Hoxa13 cause malformation of limb and genitourinary (GU) regions. In males, one of the most common GU malformations associated with loss of Hoxa13 function is hypospadia,a condition defined by the poor growth and closure of the urethra and glans penis. By examining early signaling in the developing mouse genital tubercle,we show that Hoxa13 is essential for normal expression of Fgf8 and Bmp7 in the urethral plate epithelium. In Hoxa13GFP-mutant mice, hypospadias occur as a result of the combined loss of Fgf8 and Bmp7 expression in the urethral plate epithelium, as well as the ectopic expression of noggin(Nog) in the flanking mesenchyme. In vitro supplementation with Fgf8 restored proliferation in homozygous mutants to wild-type levels, suggesting that Fgf8 is sufficient to direct early proliferation of the developing genital tubercle. However, the closure defects of the distal urethra and glans can be attributed to a loss of apoptosis in the urethra,which is consistent with reduced Bmp7 expression in this region. Mice mutant for Hoxa13 also exhibit changes in androgen receptor expression, providing a developmental link between Hoxa13-associated hypospadias and those produced by antagonists to androgen signaling. Finally,a novel role for Hoxa13 in the vascularization of the glans penis is also identified.

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Section: Hoxa13 Is Essential For Maintaining Capillary Vessel Diametementioning

confidence: 99%

Loss of Bmp7 and Fgf8 signaling in Hoxa13-mutant mice causes hypospadia

et al. 2003

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“…Hexazinone concentrations were based on preliminary studies (Nieves-Puigdoller and McCormick, unpublished data) where no significant effect of HEX was found on survival, ion regulation and plasma hormones of Atlantic salmon smolts exposed to environmentally relevant concentrations of 2, 20 and 200 g l −1 HEX. Fish were exposed to chemicals for 21 days, a time period that has been widely used as a proxy for long-term exposure (Davies et al, 1994;Baatrup and Junge, 2001;Spano et al, 2004;Lerner et al, 2007). Exposure began approximately 1-month prior to the beginning of normal downstream migration and the peak of smolt development based on previous lab studies (McCormick et al, 1995).…”

Section: Chemical Exposurementioning

confidence: 99%

Effects of hexazinone and atrazine on the physiology and endocrinology of smolt development in Atlantic salmon

2007

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Exposure to hexazinone (HEX) and atrazine (ATZ), highly mobile and widely used herbicides along rivers in the United States, is potentially harmful to Atlantic salmon, which have been listed as an endangered species. To determine the effects of these contaminants on smolt development, juvenile Atlantic salmon were exposed under flow-through conditions to 100 g l −1 HEX, 10 and 100 g l −1 ATZ in fresh water (FW) for 21 days at 10 • C beginning in mid-April. Twelve fish per treatment were sampled in FW, following a 24 h seawater (SW) challenge and after growth for 3 months in SW. Exposure to 100 g l −1 HEX or 10 g l −1 ATZ caused no mortalities of smolts in FW or after SW challenge, while 9% of the fish exposed to 100 g l −1 ATZ died during exposure. Fish exposed to 100 g l −1 ATZ reduced feeding after 10 days of exposure and had an impaired growth rate in FW and during the first month in SW; compensatory growth occurred in the second and third month in SW. HEX and ATZ at 10 g l −1 exposure had no effect on plasma levels of cortisol, growth hormone (GH), insulin growth factor I (IGF-I), thyroxine (T 4 ) and plasma 3,5,3 -triiodo-l-thyronine (T 3 ), Cl − , Mg 2+ , Na + , Ca 2+ in FW or after SW challenge. FW smolts exposed to 100 g l −1 ATZ had decreased plasma Cl − , Mg 2+ , Na + and Ca 2+ ions and increased cortisol. No effect on plasma levels of GH, IGF-I, T 4 or T 3 was found in FW smolts exposed to 100 g l −1 ATZ. Following SW challenge, fish previously exposed to 100 g l −1 ATZ had significant increases in hematocrit, plasma cortisol, Cl − , Mg 2+ , Na + , Ca 2+ and a decrease in T 4 and T 3 . It is concluded that under the conditions imposed in this study, HEX does not affect salinity tolerance of Atlantic salmon smolts, while ATZ causes ionoregulatory, growth and endocrine disturbance.

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“…Furthermore, poeciliids have several readily measurable sexual characteristics that are affected by exposure to chemicals with estrogenic or antiandrogenic activity (Baatrup and Junge 2001;Bayley et al 1999;Dreze et al 2000;Haubruge et al 2000;Toft and Baatrup 2001). Male mosquitofish have mature sperm cells in their testes year-round (Fraile et al 1992), and they have an extended reproductive period dependent on photoperiod and temperature (Fraile et al 1994;Koya et al 1998).…”

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confidence: 99%

Disturbed sexual characteristics in male mosquitofish (Gambusia holbrooki) from a lake contaminated with endocrine disruptors.

Toft

Edwards

Baatrup

et al. 2003

Environ Health Perspect

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Previous laboratory studies have demonstrated that estrogenic and antiandrogenic chemicals can alter several sexual characteristics in male poeciliid fishes. Whether similar disturbances occur under field conditions remains to be confirmed. Lake Apopka, Florida, is contaminated with numerous chemicals, some of which possess endocrine-disrupting activity. Male mosquitofish (Gambusia holbrooki) were collected monthly from December 2000 through May 2001 from Lake Apopka and two nearby reference lakes, Orange Lake and Lake Woodruff National Wildlife Refuge. Selected sexual characteristics were compared temporally and among lakes during the collection period. Male fish from Lake Apopka had slightly shorter gonopodia and on average 32 and 47% fewer sperm cells per milligram testis, when compared with the fish collected from Orange Lake and Lake Woodruff, respectively. The testes weights increased markedly during spring, with significantly smaller testes in fish from Lake Apopka than from Orange Lake, but surprisingly, the smallest testes occurred in males obtained from the Lake Woodruff population. The highest liver weights were found in the Lake Apopka population. Whole-body concentrations of testosterone and estradiol varied among months; the peak testosterone concentration occurred in January and was significantly lower in male fish from Lake Apopka compared with Orange Lake. The intensity of male courtship behavior was highly correlated to body testosterone concentration, but no statistically significant differences in sexual behavior among the lakes were found. We conclude that sexual characteristics of relevance to male reproductive capacity are altered in the Lake Apopka mosquitofish population, and we discuss the presence of chemicals with antiandrogenic effects in Lake Apopka as a possible cause of the observed alterations.

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Antiandrogenic Pesticides Disrupt Sexual Characteristics in the Adult Male Guppy (Poecilia reticulata)

Cited by 43 publications

References 46 publications

Loss of Bmp7 and Fgf8 signaling in Hoxa13-mutant mice causes hypospadia

Loss of Bmp7 and Fgf8 signaling in Hoxa13-mutant mice causes hypospadia

Effects of hexazinone and atrazine on the physiology and endocrinology of smolt development in Atlantic salmon

Disturbed sexual characteristics in male mosquitofish (Gambusia holbrooki) from a lake contaminated with endocrine disruptors.

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