Antiallergic Asthma Properties of Brazilin through Inhibition of T_H2 Responses in T Cells and in a Murine Model of Asthma

Abstract

This study aimed to determine whether brazilin exhibits anti-inflammatory effects that inhibit T helper cell type II (T(H)2) responses and whether it suppresses allergic inflammation reactions in a murine model of asthma. We found that brazilin inhibited the mRNA and protein expression of interleukin (IL)-4 and IL-5 induced by phorbol myristate acetate (PMA) and cAMP in EL-4 T cells in a dose-dependent manner. Following the intratracheal instillation of brazilin in ovalbumin (OVA)-immunized mice, we found that brazilin-treated mice exhibited decreases in the release of IL-4, IL-5, IL-13, eotaxin-1, and tumor necrosis factor-α in bronchoalveolar lavage fluid (BALF); inhibited T(H)2 functioning via a decrease in IL-4 production; and exhibited attenuation of OVA-induced lung eosinophilia, airway hyperresponsiveness, and airway remodeling. These results suggest that brazilin exhibits anti-T(H)2 effects both in vitro and in vivo and may possess therapeutic potential for allergic diseases.

High Mobility Group Box 1 Induced Human Lung Myofibroblasts Differentiation and Enhanced Migration by Activation of MMP-9

“…Samples were loaded and separated by using 10% Tris-tricine gel with 0.1% gelatin as a substrate. After separation by electrophoresis, the gel was renatured and then incubated with development buffer at 37°C for 48 h. After development, the gel was stained with 0.5% Coomassie blue R-250 for 30 min and then destained appropriately [ 26 ]. The activity of MMP was quantified by using Image J software.…”

Brazilin plays an anti-inflammatory role with regulating Toll-like receptor 2 and TLR 2 downstream pathways in Staphylococcus aureus-induced mastitis in mice

“…1) is a compound isolated from the traditional herbal medicine Caesalpinia sappan L. Evidence has indicated that brazilin exhibits multiple biological properties, including anti-hepatotoxicity [16], anti-tumor [17], anti-platelet [18], anti-oxidant [19], immune system modulatory activities [20], and anti-inflammatory activities [21,22]. Furthermore, many studies have shown that brazilin is involved in the regulation of either NF-κB or activator protein 1 (AP-1) activation [19,23].…”

Ganoderma formosanum polysaccharides attenuate Th2 inflammation and airway hyperresponsiveness in a murine model of allergic asthma

“…Because allergic asthma is a Th2-mediated inflammatory disease, theoretically, substances that exhibit antiinflammatory and/or Th1-skewing properties could potentially be used to ameliorate the disease. Numerous herbs, phytochemicals, and vitamins have been investigated for their interactions with the immune system and used as complementary and alternative medicines in treating atopic disorders (Chang et al 2013; Chang et al 2011; Hwang et al 2012; Lee et al 2012a; Lee et al 2010; Lee et al 2012b; Mainardi et al 2009; Rao et al 2013; Wang et al 2013). Ganoderma is a medicinal fungus that is considered to be a therapeutic biofactory with numerous pharmacologically active components (Paterson 2006).…”