Antiaging Treatment of the Facial Skin by Fat Graft and Adipose-Derived Stem Cells

Charles-de-Sá, Luiz; Gontijo-de-Amorim, Natale Ferreira; Takiya, Christina Maeda; Borojević, Radovan; Benati, Donatella; Bernardi, Paolo; Sbarbati, Andrea; Rigotti, Gino

doi:10.1097/prs.0000000000001123

Cited by 181 publications

(117 citation statements)

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“…32 In this respect, lipotransfer is less expensive and time consuming and can be performed in a single surgical procedure. 7 This study provides evidence that lipotransfer restores volume and enhances facial skin texture. The study was designed to evaluate the improvements in autologous dermal white microfat transfer on facial photoaging by means of subjective and objective methods.…”

Section: Discussionmentioning

confidence: 83%

“…13 14 Several studies have been performed in order to assess the efficacy of autologous fat transplantation to promote skin rejuvenation. 6,7 Nonetheless, the results have not always been predictable since the success of the procedure is influenced by many factors, including the site and the techniques used for fat harvesting as well as the method of placement. 15 In addition, the absence of a standardized, objective, non-invasive method to evaluate the outcomes of fat transplant makes it difficult to compare results obtained following different procedures or the effects of similar methods on different patients.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

“…5 Fat transfer is rapidly becoming an emerging method among the most innovative approaches for facial rejuvenation. [6][7][8] Compared with administration of synthetic dermal fillers, fat transplant is a safer method to achieve natural-appearing volumetric rejuvenation 6 since the use of autologous material presents no biocompatibility problems. 9 Moreover, implanted adipose tissue exerts some beneficial effects on tissue surrounding the transplant area.…”

Section: Introductionmentioning

confidence: 99%

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Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Trivisonno¹,

Rossi²,

Monti³

et al. 2017

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Please cite this article as: Trivisonno, A.; Rossi, A.; Monti, M.; Di Nunno, D.; Desouches, C.; Cannistra, C.; Toietta, G. Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis. Journal of Plastic Reconstructive and Aesthetic Surgery 2017, 70, 1118-1128. DOI: 10.1016/j.bjps.2017 http://www.sciencedirect.com/science/article/pii/S1748681517301559 M A N U S C R I P T A C C E P T E D M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 2 SUMMARYCumulative, long-term exposure to solar ultraviolet radiation promotes premature skin aging characterized by wrinkle formation and reduced skin elasticity. In this study, we assessed whether microfat transfer could improve dermal and subcutaneous tissue thickness loss associated with photoaging. Twenty one patients affected by facial photoaging (photodamage grade II-IV; age range 35-62 years; 19 females, 2 males; all of Caucasian origin) were treated using minimally-invasive autologous dermal white fat transfer harvested with a recently designed microcannula. The results were determined by clinical assessment, patient self-evaluation and quantified by the Antera 3D ® dermal digital device for non-invasive, objective, reliable and accurate assessment of facial skin texture, color and wrinkle characteristics. Compared with the pretreatment condition, the increment in soft tissue volume and improvement in skin quality and texture was assessed by a dermatologist after treatment. In addition, instrumental evaluation by digital skin profilometry of the treated areas revealed:a 41% reduction in average wrinkle depth (7.29 ± 1.04 x 10 -2 mm pre-treatment vs. 4.31 ± 1.16 x 10 -2 mm at 90 days post-treatment; p < 0.001), improved skin texture, more homogeneous and uniform skin color and declined facial hemoglobin and melanin concentrations. The majority of patients (above 90%) reported improvements in self-perception. No significant complications were reported throughout the study. In conclusion, by using digital profilometry analysis as an objective and innovative tool to determine the outcome of treatment, we demonstrated that autologous microfat transfer is a safe and well-tolerated procedure with measurable beneficial effects on facial skin aging.

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Section: Discussionmentioning

confidence: 83%

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Trivisonno¹,

Rossi²,

Monti³

et al. 2017

Journal of Plastic, Reconstructive & Aesthetic Surgery

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show abstract

“…• They can secrete soluble factors that promote the effect of paracrine and immuno-modulators that facilitate therapeutic effects [4,5].…”

Section: Properties Of a Stem Cellmentioning

confidence: 99%

USE of mesenchymal stem cells extracted from the patient own adipose tissue for the treatment of degenerative pathology

Arrotegui¹

2017

Neurol Disord Therap

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“…NFSCs belong to a subcategory of adipose-derived stem cells (ASCs), which are known to participate in repair after injury, and their functions in skin rejuvenation have been described [5]. Regeneration of collagen, elastin, extra cellular matrix, and microcirculation is crucial for reversing the signs of skin aging.…”

Section: Introductionmentioning

confidence: 99%

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

Liang

Lü

et al. 2018

Cell Physiol Biochem

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Background/Aims: The rejuvenation properties of nanofat grafting have been described in recent years. However, it is not clear whether the clinical efficacy of the procedure is attributable to stem cells or linked to other components of adipose tissue. In this study we isolated nanofat-derived stem cells (NFSCs) to observe their biological characteristics and evaluate the efficacy of precise intradermal injection of nanofat combined with platelet-rich fibrin (PRF) in patients undergoing facial rejuvenation treatment. Methods: Third-passage NFSCs were isolated and cultured using a mechanical emulsification method and their surface CD markers were analyzed by flow cytometry. The adipogenic and osteogenic nature and chondrogenic differentiation capacity of NFSCs were determined using Oil Red O staining, alizarin red staining, and Alcian blue staining, respectively. Paracrine function of NFSCs was evaluated by enzyme-linked immunosorbent assay (ELISA) at 1, 3, 7, 14, and 28 days after establishing the culture. Then, the effects of PRF on NFSC proliferation were assessed in vitro. Finally, we compared the outcome in 103 patients with facial skin aging who underwent both nanofat and intradermal PRF injection (treatment group) and 128 patients who underwent hyaluronic acid (HA) injection treatment (control group). Outcomes in the two groups were compared by assessing pictures taken at the same angle before and after treatment, postoperative recovery, incidence of local absorption and cysts, and skin quality before treatment, and at 1, 12, 24 months after treatment using the VISIA Skin Image Analyzer and a SOFT5.5 skin test instrument. Results: NFSCs expressed CD29, CD44, CD49d, CD73, CD90, and CD105, but did not express CD34, CD45, and CD106. NFSCs also differentiated into adipocytes, osteoblasts, and chondrocytes under appropriate induction conditions. NFSCs released large amounts of growth factors such as VEGF, bFGF, EGF, and others, and growth factor levels increased in a time-dependent manner. At the same time, PRF enhanced proliferation of NFSCs in vitro in a dose-dependent manner, and the growth curves under different concentrations of PRF all showed plateaus 6d after seeding. Facial skin texture was improved to a greater extent after combined injection of nanofat and PRF than after control injection of HA. The nanofat-PRF group had a higher satisfaction rate. Neither treatment caused any complications such as infection, anaphylaxis, or paresthesia during long-term follow-up. Conclusion: NFSCs demonstrate excellent multipotential differentiation and paracrine function, and PRF promotes proliferation of NFSCs during the early stage after seeding. Both nanofat-PRF and HA injection improve facial skin status without serious complications, but the former was associated with greater patient satisfaction, implying that nanofat-PRF injection is a safe, highly effective, and long-lasting method for skin rejuvenation.

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Antiaging Treatment of the Facial Skin by Fat Graft and Adipose-Derived Stem Cells

Abstract: This study demonstrates that treatment with either fat and stromal vascular fraction or expanded mesenchymal stem cells modifies the pattern of the dermis, representing a skin rejuvenation effect.

Cited by 181 publications

References 34 publications

Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

Facial skin rejuvenation by autologous dermal microfat transfer in photoaged patients: Clinical evaluation and skin surface digital profilometry analysis

USE of mesenchymal stem cells extracted from the patient own adipose tissue for the treatment of degenerative pathology

Precise Intradermal Injection of Nanofat-Derived Stromal Cells Combined with Platelet-Rich Fibrin Improves the Efficacy of Facial Skin Rejuvenation

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