Antiaging Potency of Centella Asiatica Extract on Fibroblast Cells of Rattus Norvegicus Fetus by in Vitro and in Silico Approach

Abstract

Aging is a physiological process that cannot be avoided, but can be prevented by giving antioxidants. Antioxidants can be obtained from Centella asiatica extract (EkCa). EkCa has secondary metabolites that have biological effects related to inflammation and prevent aging. The purpose of this study was to determine the anti-aging potential of EkCa in fibroblast cells of Rattus norvegicus fetus and which compounds that act as anti-aging on EkCa using in vitro and in silico approach. The method used in this study was completely randomized design with 5 treatments (control, 10%, 15%, 20%, 25%), each repeated 4 times on fibroblast cells of Rattus norvegicus fetus that were 18 days pregnant. In vitro approach used to determine the confluency and viability on fibroblast cells of Rattus norvegicus while in silico approach used to screening which active compounds that have the potential as anti-aging using Way2Drug PASS online software. Data analysis used One Way ANOVA followed by Duncan's test. The results showed that the highest level of confluency and viability was found in the 25% treatment (P < 0.05) and quercetin-3-arabinoside was the compound in EkCa that has the potential as anti-aging with Pa: 0.922 and Pi: 0.003. From the result, it can be conclude that EkCa has the potential as anti-aging on fibroblast cells of Rattus norvegicus.