Antiadipogenic Effects of Red Radish (Raphanus sativus L.) Sprout Extract in 3T3-L1 Preadipocytes

Abstract

The red radish (Raphanus sativus L.; RR) sprout is a plant of the cruciferous family. In this study, we elucidated the effect of the water extract of RR sprout (RRSE) against α-amylase, α-glucosidase, and pancreatic lipase enzyme activity and adipogenesis in 3T3-L1 preadipocytes. α-amylase, αglucosidase, and pancreatic lipase enzyme activity was inhibited in a concentration-dependent manner by RRSE treatment. RSSE also abolished adipocyte differentiation and lipid and triglyceride accumulation without cytotoxicity in 3T3-L1 adipocytes. In addition, RRSE modulated the expression of the proteins related to adipogenic transcription factors: peroxisome proliferator-activated receptor (PPAR)γ, sterol regulatory element-binding protein 1 (SREBP-1), and CCAT/enhancer binding protein (C/EBP)α. RRSE also suppressed expression of the proteins responsible for lipid synthesis, transport, and storage: adiponectin, fatty acid synthesis (FAS), perilipin, and fatty acid bind protein-4 (FABP4). This study showed that RRS treatment has the potential to inhibit obesity by controlling the expression of adipogenic transcription factors and adipogenic proteins.

Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content

“…Additionally, we showed that optimally produced 2 days PRSP extract effectively inhibited MDI‐induced adipogenesis and lipogenesis. The anti‐obesity effects of RRSP have been previously reported 23‐25 . However, unlike our conditions, these studies have used RRSP extracted in water at 90 °C, 24 from which SFEN could not be thoroughly extracted or stably present, 25 by targeting other bioactive compounds such as anthocyanin in RSP rather than SFEN.…”

“…The anti‐obesity effects of RRSP have been previously reported 23‐25 . However, unlike our conditions, these studies have used RRSP extracted in water at 90 °C, 24 from which SFEN could not be thoroughly extracted or stably present, 25 by targeting other bioactive compounds such as anthocyanin in RSP rather than SFEN. Thus, this is the first time that the optimal extraction conditions have been adjusted to target ITCs including SFEN in PRSP and evaluate any anti‐obesity effects.…”

“…21 In addition to benefits for the production, RSP contains significantly higher amounts of GLSs, ITCs and phenolics than their mature root. However, although some previous studies reported the superior antioxidant activity 22 and anti-obesity effects of RSP, [23][24][25] the optimal conditions for RSP extract with high SFEN content have not yet been investigated, considering multiple variables during germination or extraction. There is still the need to explore optimal production methods of RSP extract based on bioactive compound content for practical use.…”

Hypocholesterolemic and Anti-Obesity Effects of Radish Sprouts (Raphanus Sativus) in Adult Females

“…treatment showed a significant (P<0.01) decrease in the body weight up to 5 g than the high-fat diet control group. Moreover, Kim et al (2014) stated that using RRSE of over 200 μgml -1 could inhibit the expression of adipogenic transcription factors (PPARγ, C/EBPα, and SREBP-1) and proteins (adiponectine, perilipin, FAS, and FABP4) in 3T3-L1 preadipocytes. Data from this work suggested that a consumed diet with RRS showed an anti-obesity effect and this may be attributed tothe high content of fibers and its content of phenolic compounds.…”

Pathological and biochemical evaluation of radish microgreen on diabetes and aflatoxicosis in rats

“…Red radish “ Raphanus sativus ” family “ Brassicaceae ” is a radical crop that is eaten raw, pickled, or cooked (Otsuki et al 2004 ; Tamura et al 2010 ). Red radish is rich in important vitamins and minerals (folic acid), anthocyanins, potassium (K), calcium (Ca), copper (Cu), iron (Fe), phosphorus (P), zinc (Zn), anthocyanins, fiber, and antioxidants making it a potential candidate in avoiding cardiovascular diseases, dyslipidemic conditions, and hypoglycemic influences (Kim et al 2014 ; Anna et al 2016 ; Khedr and El Sheikh 2016 ). Vivarelli et al ( 2016 ) suggested that radish juice could be a good nutraceutical product with efficient antioxidant, hypolipidemic, and anti-obesity effects.…”