Anti-γδ T cell antibody blocks the induction and maintenance of oral tolerance to ovalbumin in mice

Mengel, José; Cardillo, Fabı́ola; Aroeira, Luiz S.; Williams, Owen; Russo, Marco; Vaz, Nelson M.

doi:10.1016/0165-2478(95)02451-4

Cited by 110 publications

(64 citation statements)

References 10 publications

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“…The mechanism for the decrease of TCRgd-T cells by the antibody treatment seems to be down regulation of TCR expression on TCRgd-T cells or the depletion of those cells by complement-dependent cytolysis. 17) Oral sensitization of WBB6F1-(ϩ/ϩ) mice after the antiTCRgd antibody treatment yielded the following findings: (i) increases in antigen-specific antibody titers in the antiTCRgd antibody-treated mice (Table 1), (ii) hypothermia after i.p. antigen challenge, demonstrating ASA induction (Fig.…”

Section: Discussionmentioning

confidence: 99%

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The Hyperresponsiveness of W/Wv Mice to Oral Sensitization Is Associated with a Decrease in TCR.GAMMA..DELTA.-T Cells

Okunuki

Teshima

Sato

et al. 2005

Biological & Pharmaceutical Bulletin

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The number of patients with food allergies has been increasing for various reasons (e.g., changes in diet, increased stress, etc.), but the mechanisms of the development and regulation of food allergy have not yet been fully elucidated. Thus, oral sensitization models in animals are important for better understanding the induction and regulation mechanisms of food allergy. [1][2][3] When the allergenicity of dietary proteins is evaluated in animal, it is desirable to perform the sensitization orally, preferably without using adjuvants. However, since orally administered food antigens induce tolerance by various mechanisms, [4][5][6] it is important to optimize the dosage of the allergen and the duration of immunization in order to achieve oral sensitization without inducing tolerance.In previous studies, we compared animal food allergy models using various strains of mice and rat, 7) and found that significant antigen-specific antibody production was successfully induced without any adjuvant by orally administering OVA for 9 weeks in B10A mice and BALB/c mice. More recently, W/W v mice have been proven to be a good responder to food allergens, because their antigen-specific antibody titer was much higher than in the other murine strains used. 8)It is important to clarify the cause of the high susceptibility of the W/W v mice to oral sensitization in order to elucidate the mechanism of development of food allergy.W/W v mice have mutations in the c-kit gene and exhibit defects or a deficiency of mast cells, red blood cells, and interstitial cells of Cajal, which ordinarily express c-kit protein.9,10) The c-kit protein is a member of the type III receptor tyrosine kinase family, and its dimer is a ligand for the stem cell factor (SCF).11) We have reported low susceptibility of the congenic wild-type (ϩ/ϩ) mice to oral sensitization. The lack of high sensitivity in c-kit-positive wild-type (ϩ/ϩ) mice suggested that the susceptibility of W/W v mice to oral sensitization is related to the mutations in the c-kit gene.12)The population of gd-IELs is much smaller in W/W v mice than in wild-type mice.12) Moreover, it has been demonstrated that the ratio of the gd-IELs to total T cells in mouse intestinal mucosa is much higher than in other peripheral lymphoid tissues, 13) and that gd-IELs have been found to develop thymus independently in cryptopatches (CPs), which are located in the lamina propria. CPs are the small clusters of immature lymphocytes (c-kitϩ, IL-7Rϩ, Thy-1ϩ, LFA-1ϩ).14,15) Thus, gd-IELs are thought to play an important role in immune responses in the mucosa, although their function in oral sensitization is not fully understood. 16)The objective of this study was to identify the cause of the high susceptibility of W/W v mice to oral sensitization, and we performed two experiments to achieve this objective. In the first experiment, congenic wild-type (ϩ/ϩ) mice were treated with the anti-TCRgd antibody before oral sensitization to OVA. In the second experiment, bone marrow cell-reconstituted W/W v mice with ...

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Section: Discussionmentioning

confidence: 99%

“…injections. 17) From the day 7 to the day 70, the mice Hematological Examination Red blood cell (RBC) counts, white blood cell (WBC) counts, platelet (PLT) counts, hemoglobin (HGB) volumes, and hematocrits (HCT) were determined with an automatic hematology analyzer (M-2000, Sysmex corp., Japan).…”

Section: Methodsmentioning

confidence: 99%

The Hyperresponsiveness of W/Wv Mice to Oral Sensitization Is Associated with a Decrease in TCR.GAMMA..DELTA.-T Cells

Okunuki

Teshima

Sato

et al. 2005

Biological & Pharmaceutical Bulletin

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“…Approximately 70% of these ␥␦ T cells coexpress V␥2 and V␦2 TCR chains, and are uniformly reactive to nonpeptide organophosphate and alkylamine Ag, without CD1 or MHC restriction (35,36). Several lines of evidence suggest that these cells play an important role in immunoregulation (37)(38)(39)(40)(41)(42)(43). ␥␦ T cells prime macrophages to produce TNF-␣ in response to LPS stimulation (43), and augment production of macrophage-derived NO (44).…”

Section: Human V␥2v␦2 T Cells Augment Migration-inhibitory Factor Secmentioning

confidence: 99%

Human Vγ2Vδ2 T Cells Augment Migration-Inhibitory Factor Secretion and Counteract the Inhibitory Effect of Glucocorticoids on IL-1β and TNF-α Production

Wang

Das

Kamath

et al. 2002

The Journal of Immunology

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In immune cells, proinflammatory cytokine gene expression is regulated by glucocorticoids, whereas migration-inhibitory factor (MIF), a pleiotropic cytokine, has the unique property of counteracting the inhibitory effect of glucocorticoids on TNF-α and IL-1β secretion. A few lines of evidence suggest that γδ T cells play an important role in immunoregulation. However, it is unknown whether human γδ T cells participate in regulating MIF secretion, and how γδ T cells, glucocorticoids, and cytokines converge to give a unified physiological response. In this study, we demonstrate that human Vγ2Vδ2 T cells augment MIF secretion. Remarkably, these Vγ2Vδ2 T cells, functioning similarly to MIF in part, counteracted inhibition of dexamethasone on production of IL-1β and TNF-α. SCID mice reconstituted with human PBMC that were mock depleted of Vδ2 T cells and repeatedly infected with lethal dose of Escherichia coli had shorter survival time than those reconstituted with PBMC that were depleted of Vδ2 T cells. Thus, human Vγ2Vδ2 T cells are likely to play broad-spectrum roles in immunoregulation and immunopathology by influencing MIF secretion and the immunomodulatory function of glucocorticoids.

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“…Moreover, T-cell receptor (TCR) cd-expressing CD8z T-cells have been shown to play an important role, since IgE responses to inhaled antigens in rodent models of nasal or bronchial induced tolerance could be suppressed by the transfer of antigen-specific cd z T-cells [141]. Similarly, in vivo treatment with specific anti-TCRcd antibodies inhibited the induction of oral tolerance in ovalbumin-fed mice [142]. Conversely, FUJIHASHI et al [143] reported an abrogation of oral tolerance by cd -T-cells from the gut epithelium.…”

Section: Mucosal Tolerancementioning

confidence: 99%

Lung mucosal immunity: immunoglobulin-A revisited

et al. 2001

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Mucosal defence mechanisms are critical in preventing colonization of the respiratory tract by pathogens and penetration of antigens through the epithelial barrier. Recent research has now illustrated the active contribution of the respiratory epithelium to the exclusion of microbes and particles, but also to the control of the inflammatory and immune responses in the airways and in the alveoli. Epithelial cells also mediate the active transport of polymeric immunoglobulin-A from the lamina propria to the airway lumen through the polymeric immunoglobulin receptor. The role of IgA in the defence of mucosal surfaces has now expanded from a limited role of scavenger of exogenous material to a broader protective function with potential applications in immunotherapy. In addition, the recent identification of receptors for IgA on the surface of blood leukocytes and alveolar macrophages provides an additional mechanism of interaction between the cellular and humoral immune systems at the level of the respiratory tract.

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Anti-γδ T cell antibody blocks the induction and maintenance of oral tolerance to ovalbumin in mice

Cited by 110 publications

References 10 publications

The Hyperresponsiveness of W/Wv Mice to Oral Sensitization Is Associated with a Decrease in TCR.GAMMA..DELTA.-T Cells

The Hyperresponsiveness of W/Wv Mice to Oral Sensitization Is Associated with a Decrease in TCR.GAMMA..DELTA.-T Cells

Human Vγ2Vδ2 T Cells Augment Migration-Inhibitory Factor Secretion and Counteract the Inhibitory Effect of Glucocorticoids on IL-1β and TNF-α Production

Lung mucosal immunity: immunoglobulin-A revisited

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