Anti-β2-glycoprotein I antibodies of IgM class are linked to thrombotic disorders in young women without autoimmune disease

Salobir, Barbara; Šabovič, Mišo; Hojnik, Maja; Čučnik, Saša; Kveder, T

doi:10.1016/j.imbio.2007.01.001

Cited by 6 publications

(3 citation statements)

References 36 publications

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“…The mechanism in APS is thought to be the generation of cross-reactive B cells. In fact, it has been demonstrated that immunization with foreign P20PI induces production of a high titre of anti-p2GPI antibodies as well as development of clinical features of APS in normal mice (24), however, their exact prevalence and role in the pathogenesis of thromboses in the absence of autoimmune disease is still inconclusive (25). A recent study reported that the titres of anti-p2GPI were not different between patients with coronary artery disease and healthy subjects because most of subjects in both groups had levels of antibodies within the normal range (26).…”

Section: Anti-~2gpi and Acl Antibodies In Sera Ofpatients And Healthymentioning

confidence: 99%

Beta2-Glycoprotein I is a Target of T Cell Reactivity in Patients with Advanced Carotid Atherosclerotic Plaques

Profumo

Buttari

Alessandri

et al. 2010

Int J Immunopathol Pharmacol

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Evidence in animal models that beta2-glycoprotein I (beta2GPI), the principal target of autoimmune antiphospholipid antibodies, is involved in the initiation and progression of atherosclerosis, prompted us to investigate the possible role of this self protein as a target autoantigen of immune reactions in patients with carotid atherosclerosis. Plaque-infiltrating T lymphocytes from patients, and circulating T lymphocytes from patients and healthy subjects were tested by cell proliferation assay and by flow cytometry for intracellular cytokine expression in response to beta2GPI. ELISA was used to detect cytokine production in culture supernatants and anti-beta2GPI/anti-cardiolipin antibodies in serum samples. Eight of 35 PBMC samples and 1 of 5 plaque-infiltrating T lymphocyte samples from patients proliferated in response to beta2GPI, whereas PBMC from healthy subjects did not. Patients PBMC samples that proliferated in response to beta2GPI produced significantly higher IFN-gamma and TNF-alpha than non-proliferating PBMC. beta2GPI-specific plaque-derived T lymphocytes expressed IFN-gamma, TNF-alpha and IL-4, suggesting concomitant Th1 and Th2 activation. Only one patients serum was positive for anti-beta2GPI and anti-cardiolipin IgM antibodies. These new findings indicate that beta2GPI induces a cellular immune response in a subpopulation of patients with carotid atherosclerosis thus contributing to the inflammatory responses involved in carotid atherosclerotic disease.

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Section: Anti-~2gpi and Acl Antibodies In Sera Ofpatients And Healthymentioning

confidence: 99%

Beta2-Glycoprotein I is a Target of T Cell Reactivity in Patients with Advanced Carotid Atherosclerotic Plaques

Profumo

Buttari

Alessandri

et al. 2010

Int J Immunopathol Pharmacol

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show abstract

“…These data suggest that the IgM anti-β2-gpI autoantibody could behave differently from the IgA isotype as regard to MetS. aCL antibody testing resulted negative in the majority of these studies [23][24][25][26][27][28] and also in our patients with MetS. Altogether, these findings did not show a relevant role for aCL antibodies in patients with atherosclerotic disease or MetS.…”

Section: Discussionmentioning

confidence: 56%

“…Of interest, IgM anti-β2-gpI antibodies were linked to thrombotic disorders in young women without autoimmune disease, particularly when classical risk factors or MetS were absent; the IgA isotype was not tested in this study 28 . These data suggest that the IgM anti-β2-gpI autoantibody could behave differently from the IgA isotype as regard to MetS.…”

Section: Discussionmentioning

confidence: 99%