Anti β2-glycoprotein I antibodies: Clinical significance

Tincani, Anǵela; Spatola, L; Cinquini, Massimo; Cattaneo, Roberto; Meroni, Pier Luigi; Balestrieri, G.

doi:10.1177/096120339800700224

Cited by 15 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…POAG patients have significantly increased b2AGP concentrations, and the number of patients with elevated concentrations is significantly increased. The identification of b2AGP as an essential component of the antigenic complex recognized by ACL suggested that this molecule could be a direct target of the antibody response [15]. Our findings contrast with the results of Tsakiris et al [16] who found comparable b2AGP levels in NTG patients, chronic POAG patients and controls.…”

Section: Discussioncontrasting

confidence: 99%

Antiphosphatidylserine antibodies are elevated in normal tension glaucoma

Kremmer¹,

Kreuzfelder

Klein

et al. 2001

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThe two main entities of open-angle glaucoma are primary open-angle glaucoma (POAG) and normal tension glaucoma (NTG). Both diseases may be associated with autoimmune processes. Therefore, IgG and IgM antibodies to phospholipids (APL) and their subspecies cardiolipin (ACL), phosphatidylserine (APS) and b2-glycoprotein (b 2GP) were determined in 43 NTG patients, 40 POAG patients and 40 healthy controls in a prospective study. The most prominent observation was the increase in APS concentrations in NTG patients (IgG 20´6^2´7 U/ml, IgM 24´4^3´4 U/ml) compared with POAG

show abstract

Section: Discussioncontrasting

confidence: 99%

Antiphosphatidylserine antibodies are elevated in normal tension glaucoma

Kremmer¹,

Kreuzfelder

Klein

et al. 2001

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…This is the first study to report the aPL isotype distribution of aβ2‐GPI antibody subtypes IgG, IgM and IgA in an APS cohort. Although aβ2‐GPI is not included in the diagnostic criteria of APS, it has been realized in recent years that aβ2‐GPI confers an overall better specificity in the clinical evaluation of patients with APS and a higher positive predictive value 11,12 . There is also a certain subset of seronegative APS patients who are only positive for aβ2‐GPI 13 .…”

Section: Discussionmentioning

confidence: 99%

Antiphospholipid syndrome in Asians: clinical manifestations, serological markers and outcome of the National University of Singapore/National University Hospital antiphospholipid cohort

Yoon

Fong

Sivalingam

et al. 2003

APLAR Journal of Rheumatology

View full text Add to dashboard Cite

Background: There is a paucity of studies on antiphospholipid syndrome (APS) in Asian patients. Aim: This is the first study in Singapore to describe the clinical features, serological markers and outcomes of one of the largest cohort of APS patients in South‐east Asia. Method: One hundred and forty‐six patients were studied. Results: Within the study group, 89 were primary APS and 57 secondary APS. Age range: 16–84 years (mean Age: 50.1 years). Male : female ratio: 1 : 2.1. Racial distribution: Chinese, 69.2%; Malays, 18.5%; Indians, 11.7%. Systemic lupus erythematosus was the commonest disease associated in 48 patients (32.9%), while 17 (11.6%) had lupus‐like disease. Eighty‐two (56.2%) patients had arterial thrombosis, 37 (25.3%) patients had venous thrombosis, 14 (9.6%) had arterial and venous thrombosis while 17 (11.6%) had obstetric manifestations; 28.8% had thrombocytopenia. Clinical manifestations were diverse with neurological (49.3%), cardiac (40%), renal (23.6%) involvement. There were also several unusual presentations. Recurrent thrombosis occurred in 24.6%. Two had catastrophic APS and there were 12 deaths (9.6%). Antiphospholipid antibody testing included lupus anticoagulant, serum aCL IgG, IgM and IgA, anti‐β2 glycoprotein I (aβ2‐GPI) IgG, IgM, and IgA. The serological positivity rates of the various antiphospholipid antibodies were reported. aβ2‐GPI was positive in 67% of those tested, of which aβ2‐GPI IgA was the most common subtype. The presence of the aβ2‐GPI was associated with recurrent venous thrombosis. Conclusion: The clinical manifestations of APS in Asian patients were diverse and multisystemic. Recurrent thrombosis rate was high with significant morbidity and mortality.

show abstract

“…Both in vitro and in vivo experimental models are highly suggestive of aPL having a direct pathogenic role in APS rather than simply being serologic markers for the disorder (4, 19). Several studies have demonstrated that the binding of pathogenic aPL to phospholipids is closely related to antibody reactivity against the β 2 GPI molecule (16, 20, 21). Not only have studies shown that anti‐β 2 GPI is a significant clinical indicator of APS (18, 22, 23), but it has also been suggested that anti‐β 2 GPI has a direct pathogenic role in APS by interfering with hemostatic reactions and with different cell types involved in both the coagulation cascade and placental implantation (4).…”

Section: Discussionmentioning

confidence: 99%

Immunization of naive BALB/c mice with human β₂‐Glycoprotein I breaks tolerance to the murine molecule

Tincani

Gilburd

Abu-Shakra

et al. 2002

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. Immunization of naive mice with ␤ 2 -glycoprotein I (␤ 2 GPI) leads to the generation of pathogenic anticardiolipin antibodies associated with clinical manifestations of the antiphospholipid syndrome (APS). The aim of this study was to determine whether immunization of naive mice with human ␤ 2 GPI, which shares homology with mouse ␤ 2 GPI molecules, breaks tolerance to murine ␤ 2 GPI and leads to the generation of anti-mouse ␤ 2 GPI.Methods. Twenty-four female BALB/c mice were immunized in the footpads with 10 g of human ␤ 2 GPI. Twelve age-and sex-matched BALB/c mice were immunized in the same manner with Freund's complete adjuvant and served as controls. The reactivity of whole sera, polyclonal IgG, and affinity-purified anti-␤ 2 GPI IgG antibodies against human, bovine, and mouse ␤ 2 GPI was evaluated by enzyme-linked immunosorbent assay.Results. High titers of anti-human ␤ 2 GPI IgG antibodies were detected 1 month after immunization. Progressively increasing titers against murine and bovine ␤ 2 GPI were recorded 1-4 months after injection. Conclusion. Immunization of mice with human␤ 2 GPI resulted in the generation of antibodies reacting with human, bovine, and murine ␤ 2 GPI. The loss of tolerance to mouse ␤ 2 GPI is attributable to the high interspecies homology of ␤ 2 GPI. These results may point to molecular mimicry as a possible cause of APS.

show abstract

Anti β2-glycoprotein I antibodies: Clinical significance

Cited by 15 publications

References 22 publications

Antiphosphatidylserine antibodies are elevated in normal tension glaucoma

Antiphosphatidylserine antibodies are elevated in normal tension glaucoma

Antiphospholipid syndrome in Asians: clinical manifestations, serological markers and outcome of the National University of Singapore/National University Hospital antiphospholipid cohort

Immunization of naive BALB/c mice with human β₂‐Glycoprotein I breaks tolerance to the murine molecule

Contact Info

Product

Resources

About

Anti β2-glycoprotein I antibodies: Clinical significance

Cited by 15 publications

References 22 publications

Antiphosphatidylserine antibodies are elevated in normal tension glaucoma

Antiphosphatidylserine antibodies are elevated in normal tension glaucoma

Antiphospholipid syndrome in Asians: clinical manifestations, serological markers and outcome of the National University of Singapore/National University Hospital antiphospholipid cohort

Immunization of naive BALB/c mice with human β2‐Glycoprotein I breaks tolerance to the murine molecule

Contact Info

Product

Resources

About

Immunization of naive BALB/c mice with human β₂‐Glycoprotein I breaks tolerance to the murine molecule